Gene description for ECT2
Gene name epithelial cell transforming 2
Gene symbol ECT2
Other names/aliases ARHGEF31
Species Homo sapiens
 Database cross references - ECT2
ExoCarta ExoCarta_1894
Entrez Gene 1894
HGNC 3155
MIM 600586
UniProt Q9H8V3  
 ECT2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ECT2
Molecular Function
    protein homodimerization activity GO:0042803 IDA
    GTPase activator activity GO:0005096 IMP
    Rho GTPase binding GO:0017048 IEA
    protein binding GO:0005515 IPI
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IDA
    signal transducer activity GO:0004871 IMP
Biological Process
    cellular response to ionizing radiation GO:0071479 IDA
    cellular response to hydrogen peroxide GO:0070301 IDA
    cellular response to calcium ion GO:0071277 IDA
    cytokinesis GO:0000910 IMP
    positive regulation of apoptotic process GO:0043065 TAS
    apoptotic signaling pathway GO:0097190 TAS
    positive regulation of neuron differentiation GO:0045666 ISS
    positive regulation of protein import into nucleus GO:0042307 ISS
    activation of GTPase activity GO:0090630 IMP
    bicellular tight junction assembly GO:0070830 IMP
    protein homooligomerization GO:0051260 IDA
    activation of protein kinase activity GO:0032147 IDA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IMP
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    protein transport GO:0015031 IEA
    regulation of protein kinase activity GO:0045859 IDA
    small GTPase mediated signal transduction GO:0007264 TAS
    positive regulation of cytokinesis GO:0032467 IMP
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 IMP
    positive regulation of GTPase activity GO:0043547 IMP
    cell morphogenesis GO:0000902 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    cell-cell junction GO:0005911 IDA
    cleavage furrow GO:0032154 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    bicellular tight junction GO:0005923 IDA
    cytoplasm GO:0005737 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    midbody GO:0030496 IDA
    nuclear membrane GO:0031965 IDA
    centralspindlin complex GO:0097149 IDA
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified ECT2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ECT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PARD6A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 PARD3 56288
Affinity Capture-Western Homo sapiens
3 PRKCZ 5590
Affinity Capture-Western Homo sapiens
4 KLHL20  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ECT2 is involved
PathwayEvidenceSource
G alpha (12/13) signalling events TAS Reactome
NRAGE signals death through JNK TAS Reactome
Rho GTPase cycle TAS Reactome





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