Gene description for GFAP
Gene name
glial fibrillary acidic protein
Gene symbol
GFAP
Other names/aliases
ALXDRD
Species
Homo sapiens
Database cross references - GFAP
ExoCarta
ExoCarta_2670
Entrez Gene
2670
HGNC
4235
MIM
137780
UniProt
P14136
GFAP identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells
25944692
Squamous carcinoma cells
20124223
Gene ontology annotations for GFAP
Experiment description of studies that identified GFAP in exosomes
1
Experiment ID
224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
2
Experiment ID
191
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for GFAP
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
S100A1
Affinity Capture-MS
Homo sapiens
2
PSEN2
Two-hybrid
Homo sapiens
3
RIBC2
Two-hybrid
Homo sapiens
4
GRAP2
9402
Two-hybrid
Homo sapiens
5
C18orf9
Two-hybrid
Homo sapiens
6
MYO15B
Two-hybrid
Homo sapiens
7
MEN1
4221
Invivo
Homo sapiens
Invitro
Homo sapiens
Affinity Capture-MS
Homo sapiens
8
TUBGCP4
27229
Two-hybrid
Homo sapiens
9
SRC
6714
Affinity Capture-MS
Homo sapiens
10
PDZK1
5174
Two-hybrid
Homo sapiens
11
VIM
7431
Two-hybrid
Homo sapiens
12
PDLIM7
9260
Two-hybrid
Homo sapiens
13
POM121
Two-hybrid
Homo sapiens
14
PDLIM1
9124
Two-hybrid
Homo sapiens
15
KIAA0408
Two-hybrid
Homo sapiens
16
PSEN1
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
17
S100B
Affinity Capture-MS
Homo sapiens
18
SH3YL1
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which GFAP is involved