Gene description for DST
Gene name dystonin
Gene symbol DST
Other names/aliases BP240
BPA
BPAG1
CATX-15
CATX15
D6S1101
DMH
DT
EBSB2
HSAN6
MACF2
Species Homo sapiens
 Database cross references - DST
ExoCarta ExoCarta_667
Vesiclepedia VP_667
Entrez Gene 667
HGNC 1090
MIM 113810
UniProt Q03001  
 DST identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for DST
Molecular Function
    actin binding GO:0003779 IEA
    integrin binding GO:0005178 IPI
    structural molecule activity GO:0005198 IBA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IBA
    protein homodimerization activity GO:0042803 IDA
    microtubule plus-end binding GO:0051010 IDA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IDA
    cytoskeleton organization GO:0007010 IMP
    cell adhesion GO:0007155 IEA
    integrin-mediated signaling pathway GO:0007229 NAS
    retrograde axonal transport GO:0008090 ISS
    response to wounding GO:0009611 IDA
    maintenance of cell polarity GO:0030011 IMP
    hemidesmosome assembly GO:0031581 IDA
    wound healing GO:0042060 IBA
    intermediate filament cytoskeleton organization GO:0045104 IBA
    intermediate filament cytoskeleton organization GO:0045104 IEP
    intermediate filament cytoskeleton organization GO:0045104 NAS
    cell motility GO:0048870 IMP
Subcellular Localization
    stress fiber GO:0001725 IEA
    basement membrane GO:0005604 TAS
    nucleus GO:0005634 HDA
    nuclear envelope GO:0005635 IEA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 IBA
    focal adhesion GO:0005925 HDA
    focal adhesion GO:0005925 IBA
    cell cortex GO:0005938 IEA
    basal plasma membrane GO:0009925 NAS
    actin cytoskeleton GO:0015629 IDA
    actin cytoskeleton GO:0015629 IDA
    microtubule cytoskeleton GO:0015630 IDA
    membrane GO:0016020 IBA
    Z disc GO:0030018 IDA
    Z disc GO:0030018 IEA
    hemidesmosome GO:0030056 IBA
    hemidesmosome GO:0030056 IDA
    hemidesmosome GO:0030056 TAS
    axon GO:0030424 IDA
    cell leading edge GO:0031252 IDA
    cytoplasmic vesicle GO:0031410 IDA
    H zone GO:0031673 IEA
    microtubule plus-end GO:0035371 IDA
    intermediate filament cytoskeleton GO:0045111 IDA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified DST in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DST
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 AFG3L2 10939
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 LCK 3932
Proximity Label-MS Homo sapiens
5 CEP250 11190
Cross-Linking-MS (XL-MS) Homo sapiens
6 PPP1CB 5500
Affinity Capture-MS Homo sapiens
7 IMMT 10989
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
8 EXOC1 55763
Two-hybrid Homo sapiens
9 GATSL2  
Co-fractionation Homo sapiens
10 Tpm1 22003
Affinity Capture-MS Mus musculus
11 EIF2S3 1968
Co-fractionation Homo sapiens
12 CELSR3  
Two-hybrid Homo sapiens
13 GJA1 2697
Proximity Label-MS Homo sapiens
14 PARD3 56288
Proximity Label-MS Homo sapiens
15 Calml3  
Affinity Capture-MS Mus musculus
16 APC  
Two-hybrid Homo sapiens
17 DBN1 1627
Affinity Capture-MS Homo sapiens
18 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
19 LIMA1 51474
Affinity Capture-MS Homo sapiens
20 MAGEA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 LAMP2 3920
Proximity Label-MS Homo sapiens
22 PPIA 5478
Cross-Linking-MS (XL-MS) Homo sapiens
23 CALM1 801
Reconstituted Complex Homo sapiens
24 Actb 11461
Affinity Capture-MS Mus musculus
25 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
26 STK3 6788
Affinity Capture-MS Homo sapiens
27 ZRANB1 54764
Affinity Capture-MS Homo sapiens
28 NOL8  
Cross-Linking-MS (XL-MS) Homo sapiens
29 CEACAM16  
Affinity Capture-MS Homo sapiens
30 EIF4ENIF1 56478
Two-hybrid Homo sapiens
31 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
32 RPL10A 4736
Cross-Linking-MS (XL-MS) Homo sapiens
33 SH3BP5 9467
Two-hybrid Homo sapiens
34 MLLT4 4301
Proximity Label-MS Homo sapiens
35 ATPIF1 93974
Cross-Linking-MS (XL-MS) Homo sapiens
36 MCM2 4171
Affinity Capture-MS Homo sapiens
37 ACTR3 10096
Proximity Label-MS Homo sapiens
38 IQGAP1 8826
Affinity Capture-MS Homo sapiens
39 TNIK 23043
Two-hybrid Homo sapiens
40 Mrps31  
Two-hybrid Mus musculus
41 ADD1 118
Affinity Capture-MS Homo sapiens
42 OBSL1 23363
Affinity Capture-MS Homo sapiens
43 OCLN 100506658
Proximity Label-MS Homo sapiens
44 STX6 10228
Proximity Label-MS Homo sapiens
45 MECP2 4204
Affinity Capture-MS Homo sapiens
46 RAB35 11021
Proximity Label-MS Homo sapiens
47 POTEKP 440915
Co-fractionation Homo sapiens
48 EBAG9 9166
Proximity Label-MS Homo sapiens
49 LAMTOR1 55004
Proximity Label-MS Homo sapiens
50 LMAN1 3998
Proximity Label-MS Homo sapiens
51 MTNR1B  
Two-hybrid Homo sapiens
52 UNK  
Affinity Capture-RNA Homo sapiens
53 ATG9A 79065
Proximity Label-MS Homo sapiens
54 MYO18A 399687
Affinity Capture-MS Homo sapiens
55 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
56 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
57 CXADR 1525
Proximity Label-MS Homo sapiens
58 HIST1H2AG 8969
Cross-Linking-MS (XL-MS) Homo sapiens
59 Ppp6c 67857
Affinity Capture-MS Mus musculus
60 LYN 4067
Proximity Label-MS Homo sapiens
61 MAPRE1 22919
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
62 MCAM 4162
Proximity Label-MS Homo sapiens
63 RAB4A 5867
Proximity Label-MS Homo sapiens
64 TERF1 7013
Affinity Capture-MS Homo sapiens
65 DTNBP1  
Two-hybrid Homo sapiens
66 AKR7L 246181
Affinity Capture-MS Homo sapiens
67 CDH1 999
Proximity Label-MS Homo sapiens
68 RPA2 6118
Proximity Label-MS Homo sapiens
69 DIRAS3  
Proximity Label-MS Homo sapiens
70 EPB41L4A 64097
Proximity Label-MS Homo sapiens
71 LAMP1 3916
Proximity Label-MS Homo sapiens
72 DCTN1 1639
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
73 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
74 NFYC 4802
Two-hybrid Homo sapiens
75 ACTB 60
Proximity Label-MS Homo sapiens
76 MAPRE3  
Proximity Label-MS Homo sapiens
77 Coro1c 23790
Affinity Capture-MS Mus musculus
78 ENY2 56943
Cross-Linking-MS (XL-MS) Homo sapiens
79 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
80 COPS7B 64708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 ACTC1 70
Proximity Label-MS Homo sapiens
82 RHOT2 89941
Proximity Label-MS Homo sapiens
83 PAK2 5062
Two-hybrid Homo sapiens
84 MYH9 4627
Affinity Capture-MS Homo sapiens
85 VSX1  
Affinity Capture-MS Homo sapiens
86 UBXN6 80700
Affinity Capture-MS Homo sapiens
87 ID2  
Affinity Capture-MS Homo sapiens
88 COX4I1 1327
Cross-Linking-MS (XL-MS) Homo sapiens
89 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 DISC1 27185
Two-hybrid Homo sapiens
91 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
92 GAN 8139
Affinity Capture-MS Homo sapiens
93 MRPL12 6182
Cross-Linking-MS (XL-MS) Homo sapiens
94 MOB1B 92597
Proximity Label-MS Homo sapiens
95 Myh9 17886
Affinity Capture-MS Mus musculus
96 CEP63  
Two-hybrid Homo sapiens
97 Prkcz  
Affinity Capture-MS Mus musculus
98 RAB5A 5868
Proximity Label-MS Homo sapiens
99 ECT2 1894
Affinity Capture-MS Homo sapiens
100 SIRT7  
Affinity Capture-MS Homo sapiens
101 ERBB2IP 55914
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
102 TRAF3IP1 26146
Two-hybrid Homo sapiens
103 CTNNB1 1499
Proximity Label-MS Homo sapiens
104 MEX3A  
Affinity Capture-RNA Homo sapiens
105 CHRNA1  
Affinity Capture-MS Homo sapiens
106 COQ4  
Affinity Capture-MS Homo sapiens
107 NUP43 348995
Proximity Label-MS Homo sapiens
108 CEP290  
Cross-Linking-MS (XL-MS) Homo sapiens
109 Cep152  
Affinity Capture-MS Mus musculus
110 CDK2 1017
Affinity Capture-MS Homo sapiens
111 KCNE3  
Affinity Capture-MS Homo sapiens
112 MAPK6  
Two-hybrid Homo sapiens
113 MYC  
Affinity Capture-MS Homo sapiens
114 RPA3 6119
Proximity Label-MS Homo sapiens
115 SMAD9  
Two-hybrid Homo sapiens
116 RAB9A 9367
Proximity Label-MS Homo sapiens
117 GOLGA1  
Proximity Label-MS Homo sapiens
118 ITGB4 3691
Two-hybrid Homo sapiens
119 RPA4  
Proximity Label-MS Homo sapiens
120 BMI1  
Affinity Capture-MS Homo sapiens
121 KIAA0368 23392
Two-hybrid Homo sapiens
122 PRKAB2 5565
Two-hybrid Homo sapiens
123 NEUROG3  
Affinity Capture-MS Homo sapiens
124 COL17A1 1308
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
125 FLOT1 10211
Proximity Label-MS Homo sapiens
126 CCDC8  
Affinity Capture-MS Homo sapiens
127 TXLNA 200081
Affinity Capture-MS Homo sapiens
128 PRKD2 25865
Two-hybrid Homo sapiens
129 KIAA1549  
Two-hybrid Homo sapiens
130 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
131 TERF2  
Affinity Capture-MS Homo sapiens
132 FBXL6  
Proximity Label-MS Homo sapiens
133 PLEC 5339
Cross-Linking-MS (XL-MS) Homo sapiens
134 Myo1c 17913
Affinity Capture-MS Mus musculus
135 RDX 5962
Affinity Capture-MS Homo sapiens
136 DENND6A 201627
Proximity Label-MS Homo sapiens
137 CAPZA2 830
Affinity Capture-MS Homo sapiens
138 PRMT5 10419
Co-fractionation Homo sapiens
139 C11orf52 91894
Proximity Label-MS Homo sapiens
140 MKI67  
Affinity Capture-MS Homo sapiens
141 ITSN2 50618
Two-hybrid Homo sapiens
142 Mapre1 13589
Affinity Capture-MS Mus musculus
143 ATP5B 506
Cross-Linking-MS (XL-MS) Homo sapiens
144 RAB7A 7879
Proximity Label-MS Homo sapiens
145 PINK1  
Affinity Capture-MS Homo sapiens
146 RAB11A 8766
Proximity Label-MS Homo sapiens
147 Flot1 14251
Affinity Capture-MS Mus musculus
148 EPHA2 1969
Proximity Label-MS Homo sapiens
149 GLRX3 10539
Co-fractionation Homo sapiens
150 RPL23A 6147
Cross-Linking-MS (XL-MS) Homo sapiens
151 KRAS 3845
Proximity Label-MS Homo sapiens
152 MOB1A 55233
Proximity Label-MS Homo sapiens
153 ISL2  
Affinity Capture-MS Homo sapiens
154 PTGER4  
Two-hybrid Homo sapiens
155 CAV1 857
Proximity Label-MS Homo sapiens
156 Tecpr2  
Affinity Capture-MS Mus musculus
157 CDC5L 988
Two-hybrid Homo sapiens
View the network image/svg+xml



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