Gene description for DHX36
Gene name DEAH (Asp-Glu-Ala-His) box polypeptide 36
Gene symbol DHX36
Other names/aliases DDX36
G4R1
MLEL1
RHAU
Species Homo sapiens
 Database cross references - DHX36
ExoCarta ExoCarta_170506
Entrez Gene 170506
HGNC 14410
MIM 612767
UniProt Q9H2U1  
 DHX36 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for DHX36
Molecular Function
    DNA-dependent ATPase activity GO:0008094 IDA
    core promoter binding GO:0001047 IDA
    histone deacetylase binding GO:0042826 ISS
    G-quadruplex DNA binding GO:0051880 IDA
    transcription regulatory region DNA binding GO:0044212 ISS
    poly(A) RNA binding GO:0044822 IDA
    ATP binding GO:0005524 IEA
    ATP-dependent RNA helicase activity GO:0004004 IBA
    double-stranded RNA binding GO:0003725 IDA
    protein binding GO:0005515 IPI
    G-quadruplex RNA binding GO:0002151 IMP
Biological Process
    response to exogenous dsRNA GO:0043330 IEA
    response to virus GO:0009615 IEA
    positive regulation of telomere maintenance GO:0032206 IMP
    ossification GO:0001503 ISS
    RNA secondary structure unwinding GO:0010501 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISS
    positive regulation of type I interferon production GO:0032481 TAS
    RNA processing GO:0006396 IBA
    transcription, DNA-templated GO:0006351 IEA
    innate immune response GO:0045087 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    chromosome, telomeric region GO:0000781 IEA
    nucleus GO:0005634 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified DHX36 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DHX36
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PARVA 55742
Affinity Capture-MS Homo sapiens
2 ILK 3611
Affinity Capture-MS Homo sapiens
3 ACP6  
Affinity Capture-MS Homo sapiens
4 APG12L  
Affinity Capture-MS Homo sapiens
5 PRKAB1  
Affinity Capture-MS Homo sapiens
6 PANK2 80025
Affinity Capture-MS Homo sapiens
7 GSK3B 2932
Affinity Capture-MS Homo sapiens
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