Gene description for SRSF9
Gene name serine/arginine-rich splicing factor 9
Gene symbol SRSF9
Other names/aliases SFRS9
SRp30c
Species Homo sapiens
 Database cross references - SRSF9
ExoCarta ExoCarta_8683
Entrez Gene 8683
HGNC 10791
MIM 601943
UniProt Q13242  
 SRSF9 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for SRSF9
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
Biological Process
    transcription from RNA polymerase II promoter GO:0006366 TAS
    RNA splicing GO:0008380 TAS
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    mRNA splice site selection GO:0006376 TAS
    mRNA processing GO:0006397 TAS
    mRNA export from nucleus GO:0006406 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    gene expression GO:0010467 TAS
    mRNA 3'-end processing GO:0031124 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified SRSF9 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SRSF9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C1QBP 708
Far Western Homo sapiens
2 YBX1 4904
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 NOL3  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 KHDRBS3  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 YT521  
Invivo Homo sapiens
6 SAFB  
Invivo Homo sapiens
7 TRA2B 6434
Reconstituted Complex Homo sapiens
8 DUSP11  
Two-hybrid Homo sapiens
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