Experiment description of studies that identified ATAD3A in exosomes

1 Experiment ID 412 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX|ACTB Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

2 Experiment ID 414 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

3 Experiment ID 426 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 427 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 407 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

6 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

9 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

10 Experiment ID 405 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Foreskin fibroblasts Sample name BJ Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

11 Experiment ID 417 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX|ACTB Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Lymphoma cells Sample name Raji Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

12 Experiment ID 411 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Mammary cancer-associated fibroblasts Sample name mCAF Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

13 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

14 Experiment ID 418 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Monocytic leukemia cells Sample name THP-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

15 Experiment ID 418 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Monocytic leukemia cells Sample name THP-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

16 Experiment ID 413 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Normal mammary epithelial cells Sample name MCF10A Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

17 Experiment ID 406 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name BxPC3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

18 Experiment ID 415 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

19 Experiment ID 434 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

20 Experiment ID 435 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

21 Experiment ID 408 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX|ACTB Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic duct epithalial cells Sample name HPDE Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

22 Experiment ID 409 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic duct epithalial cells Sample name HPNE Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

23 Experiment ID 416 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pluripotent stem cells Sample name PSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

24 Experiment ID 191 MISEV standards ✘ Biophysical techniques ✔ Alix|CD81|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20124223     Organism Homo sapiens Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK." Journal name Mol Cell Proteomics Publication year 2010 Sample Squamous carcinoma cells Sample name Squamous carcinoma cell (A431) Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

25 Experiment ID 410 MISEV standards ✔ EM Biophysical techniques ✔ CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample T lymphocytes Sample name Jurkat Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

Protein-protein interactions for ATAD3A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 2 UBE2H 7328 Affinity Capture-MS Homo sapiens 3 PMPCA 23203 Proximity Label-MS Homo sapiens 4 FMNL1 752 Affinity Capture-MS Homo sapiens 5 SPRTN   Affinity Capture-MS Homo sapiens 6 MTCH1 23787 Proximity Label-MS Homo sapiens 7 LGR4 55366 Affinity Capture-MS Homo sapiens 8 COIL   Proximity Label-MS Homo sapiens 9 COX15 1355 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 10 CSNK2A2 1459 Affinity Capture-MS Homo sapiens 11 KCTD10 83892 Affinity Capture-MS Homo sapiens 12 CSNK1A1 1452 Affinity Capture-MS Homo sapiens 13 FBXO25   Affinity Capture-MS Homo sapiens 14 TLX3   Affinity Capture-MS Homo sapiens 15 ACTC1 70 Affinity Capture-MS Homo sapiens 16 SOX2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 KLF8   Affinity Capture-MS Homo sapiens 18 KIF20A 10112 Affinity Capture-MS Homo sapiens 19 TLX2   Affinity Capture-MS Homo sapiens 20 SRGAP2 23380 Affinity Capture-MS Homo sapiens 21 NDUFA4 4697 Affinity Capture-MS Homo sapiens 22 PRPF40A 55660 Reconstituted Complex Homo sapiens 23 C15orf48   Affinity Capture-MS Homo sapiens 24 DNAJC15   Proximity Label-MS Homo sapiens 25 COX8A   Proximity Label-MS Homo sapiens 26 CCDC90B   Proximity Label-MS Homo sapiens 27 PDE2A   Affinity Capture-MS Homo sapiens 28 ATP5L 10632 Co-fractionation Homo sapiens 29 PPP1CC 5501 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 30 KIAA1429 25962 Affinity Capture-MS Homo sapiens 31 MAP3K5 4217 Affinity Capture-MS Homo sapiens 32 S100B 6285 Affinity Capture-MS Homo sapiens 33 MCU 90550 Proximity Label-MS Homo sapiens 34 B3GNT2 10678 Affinity Capture-MS Homo sapiens 35 KIF23 9493 Affinity Capture-MS Homo sapiens 36 MCM2 4171 Affinity Capture-MS Homo sapiens 37 PHB2 11331 Proximity Label-MS Homo sapiens Co-fractionation Homo sapiens 38 PTPMT1 114971 Affinity Capture-MS Homo sapiens 39 RPN1 6184 Proximity Label-MS Homo sapiens Co-fractionation Homo sapiens 40 HINT2 84681 Proximity Label-MS Homo sapiens 41 NPRL2   Affinity Capture-MS Homo sapiens 42 COQ9   Affinity Capture-MS Homo sapiens 43 CEMIP 57214 Affinity Capture-MS Homo sapiens 44 FBXW8 26259 Affinity Capture-MS Homo sapiens 45 KLF15   Affinity Capture-MS Homo sapiens 46 OBSL1 23363 Affinity Capture-MS Homo sapiens 47 PPEF1   Affinity Capture-MS Homo sapiens 48 SLC25A12 8604 Proximity Label-MS Homo sapiens 49 SURF1   Proximity Label-MS Homo sapiens 50 RPN2 6185 Proximity Label-MS Homo sapiens 51 C20orf24   Affinity Capture-MS Homo sapiens 52 ITGA4 3676 Affinity Capture-MS Homo sapiens 53 VDAC1 7416 Affinity Capture-MS Homo sapiens 54 SLC25A46 91137 Proximity Label-MS Homo sapiens 55 KLF4   Affinity Capture-MS Homo sapiens 56 VCAM1 7412 Affinity Capture-MS Homo sapiens 57 CEP57   Affinity Capture-MS Homo sapiens 58 PMS2   Affinity Capture-MS Homo sapiens 59 MECP2 4204 Affinity Capture-MS Homo sapiens 60 PXMP2   Proximity Label-MS Homo sapiens 61 CUL1 8454 Affinity Capture-MS Homo sapiens 62 TFCP2 7024 Affinity Capture-MS Homo sapiens 63 MYCN   Affinity Capture-MS Homo sapiens 64 SSR1 6745 Co-fractionation Homo sapiens 65 SOX5 6660 Affinity Capture-MS Homo sapiens 66 Spag5   Affinity Capture-MS Mus musculus 67 MARCH5   Proximity Label-MS Homo sapiens 68 SLC25A3 5250 Co-fractionation Homo sapiens 69 KIF14 9928 Affinity Capture-MS Homo sapiens 70 SFXN1 94081 Proximity Label-MS Homo sapiens 71 HSPA8 3312 Affinity Capture-MS Homo sapiens 72 AMBRA1   Affinity Capture-Western Homo sapiens 73 MDH2 4191 Proximity Label-MS Homo sapiens 74 CPEB1   Affinity Capture-MS Homo sapiens 75 KLF16   Affinity Capture-MS Homo sapiens 76 APOO 79135 Proximity Label-MS Homo sapiens 77 EPB41L3 23136 Affinity Capture-MS Homo sapiens 78 NF2 4771 Affinity Capture-MS Homo sapiens 79 KLF5   Affinity Capture-MS Homo sapiens 80 PPP1CA 5499 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 81 Recql4   Affinity Capture-MS Mus musculus 82 MTCH2 23788 Proximity Label-MS Homo sapiens 83 C6orf203   Proximity Label-MS Homo sapiens 84 NDUFS4 4724 Co-fractionation Homo sapiens 85 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 86 NEB 4703 Cross-Linking-MS (XL-MS) Homo sapiens 87 FASTKD5   Proximity Label-MS Homo sapiens 88 DCBLD1   Cross-Linking-MS (XL-MS) Homo sapiens 89 PARK2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 90 PSME1 5720 Affinity Capture-MS Homo sapiens 91 MEX3C 51320 Affinity Capture-MS Homo sapiens 92 TUFM 7284 Proximity Label-MS Homo sapiens 93 COPB1 1315 Co-fractionation Homo sapiens 94 DIABLO 56616 Proximity Label-MS Homo sapiens 95 HSCB 150274 Proximity Label-MS Homo sapiens 96 PTP4A3   Affinity Capture-MS Homo sapiens 97 ESRRB   Affinity Capture-MS Homo sapiens 98 TUBA1C 84790 Affinity Capture-MS Homo sapiens 99 SREBF2 6721 Negative Genetic Homo sapiens 100 YAP1 10413 Affinity Capture-MS Homo sapiens 101 VCP 7415 Co-fractionation Homo sapiens 102 TRIM21 6737 Affinity Capture-MS Homo sapiens 103 NTRK1 4914 Affinity Capture-MS Homo sapiens 104 CCDC109B 55013 Proximity Label-MS Homo sapiens 105 OPA1 4976 Proximity Label-MS Homo sapiens 106 HUWE1 10075 Affinity Capture-MS Homo sapiens 107 CYC1 1537 Co-fractionation Homo sapiens 108 Hnrnpf 98758 Affinity Capture-MS Mus musculus 109 MYH11 4629 Cross-Linking-MS (XL-MS) Homo sapiens 110 DNAJC30   Proximity Label-MS Homo sapiens 111 TMEM70   Proximity Label-MS Homo sapiens 112 Ksr1   Affinity Capture-MS Mus musculus 113 CYLD   Affinity Capture-MS Homo sapiens 114 TNPO1 3842 Cross-Linking-MS (XL-MS) Homo sapiens 115 CCNT1   Affinity Capture-MS Homo sapiens 116 OGT 8473 Reconstituted Complex Homo sapiens 117 COX4I1 1327 Proximity Label-MS Homo sapiens 118 SP1   Affinity Capture-MS Homo sapiens 119 SEC63 11231 Affinity Capture-MS Homo sapiens 120 GAN 8139 Affinity Capture-MS Homo sapiens 121 TMPO 7112 Affinity Capture-MS Homo sapiens 122 BRF2   Affinity Capture-MS Homo sapiens 123 FANCD2   Affinity Capture-MS Homo sapiens 124 AIFM1 9131 Proximity Label-MS Homo sapiens 125 OTUD1 220213 Affinity Capture-MS Homo sapiens 126 SOX6   Affinity Capture-MS Homo sapiens 127 SLC25A51   Proximity Label-MS Homo sapiens 128 ECT2 1894 Affinity Capture-MS Homo sapiens 129 SIRT7   Affinity Capture-MS Homo sapiens 130 UQCRC2 7385 Co-fractionation Homo sapiens 131 CTNNB1 1499 Affinity Capture-MS Homo sapiens 132 CUL3 8452 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 133 NR3C1 2908 Affinity Capture-MS Homo sapiens 134 BTF3 689 Affinity Capture-MS Homo sapiens 135 ATAD3C 219293 Cross-Linking-MS (XL-MS) Homo sapiens 136 PHKG2 5261 Affinity Capture-MS Homo sapiens 137 FBXO6 26270 Affinity Capture-MS Homo sapiens 138 RIPK4   Affinity Capture-MS Homo sapiens 139 SUZ12   Affinity Capture-MS Homo sapiens 140 LARS 51520 Affinity Capture-MS Homo sapiens 141 FN1 2335 Affinity Capture-MS Homo sapiens 142 OCIAD1 54940 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 143 C17orf80 55028 Proximity Label-MS Homo sapiens 144 SOX15   Affinity Capture-MS Homo sapiens 145 AURKB 9212 Affinity Capture-MS Homo sapiens 146 AATK   Affinity Capture-MS Homo sapiens 147 PRKACA 5566 Affinity Capture-MS Homo sapiens 148 MAPK6   Affinity Capture-MS Homo sapiens 149 SOAT1 6646 Affinity Capture-MS Homo sapiens 150 MYC   Affinity Capture-MS Homo sapiens 151 RPA3 6119 Proximity Label-MS Homo sapiens 152 WWC1   Affinity Capture-MS Homo sapiens 153 DLST 1743 Affinity Capture-MS Homo sapiens 154 TSFM 10102 Proximity Label-MS Homo sapiens 155 ACAD9 28976 Proximity Label-MS Homo sapiens 156 SPATA5 166378 Affinity Capture-MS Homo sapiens 157 DDRGK1 65992 Affinity Capture-MS Homo sapiens 158 RNF2   Affinity Capture-MS Homo sapiens 159 ILK 3611 Affinity Capture-MS Homo sapiens 160 ASXL1   Affinity Capture-MS Homo sapiens 161 NEUROG3   Affinity Capture-MS Homo sapiens 162 PEX3 8504 Proximity Label-MS Homo sapiens 163 PSMD7 5713 Cross-Linking-MS (XL-MS) Homo sapiens 164 AKAP1 8165 Proximity Label-MS Homo sapiens 165 CCDC8   Affinity Capture-MS Homo sapiens 166 HIF1A 3091 Affinity Capture-MS Homo sapiens 167 DNAJC4   Proximity Label-MS Homo sapiens 168 DVL2 1856 Affinity Capture-MS Homo sapiens 169 CS 1431 Proximity Label-MS Homo sapiens Proximity Label-MS Homo sapiens 170 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 171 PPP4C 5531 Affinity Capture-MS Homo sapiens 172 TUBGCP2 10844 Affinity Capture-MS Homo sapiens 173 Tubg1 103733 Affinity Capture-MS Mus musculus 174 LAMTOR1 55004 Proximity Label-MS Homo sapiens 175 HDGF 3068 Affinity Capture-MS Homo sapiens 176 FBXL6   Affinity Capture-MS Homo sapiens 177 WWOX 51741 Affinity Capture-MS Homo sapiens 178 ZNF746   Affinity Capture-MS Homo sapiens 179 HDAC6 10013 Affinity Capture-MS Homo sapiens 180 USP36   Affinity Capture-MS Homo sapiens 181 PDHA1 5160 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 182 PPP2R2C 5522 Affinity Capture-MS Homo sapiens 183 MLH1 4292 Affinity Capture-MS Homo sapiens 184 FASN 2194 Negative Genetic Homo sapiens 185 NDUFB8 4714 Co-fractionation Homo sapiens 186 MKI67   Affinity Capture-MS Homo sapiens 187 CUL9   Affinity Capture-MS Homo sapiens 188 ESR1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 189 EXD2   Proximity Label-MS Homo sapiens 190 UFL1 23376 Affinity Capture-MS Homo sapiens 191 LONP1 9361 Proximity Label-MS Homo sapiens 192 COQ2   Affinity Capture-MS Homo sapiens 193 CHMP4C 92421 Affinity Capture-MS Homo sapiens 194 AARS2   Proximity Label-MS Homo sapiens 195 PINK1   Affinity Capture-MS Homo sapiens 196 RNPS1 10921 Cross-Linking-MS (XL-MS) Homo sapiens 197 C1QBP 708 Proximity Label-MS Homo sapiens 198 SERBP1 26135 Affinity Capture-MS Homo sapiens 199 ATAD3B 83858 Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 200 CUL7 9820 Affinity Capture-MS Homo sapiens 201 Shcbp1 20419 Affinity Capture-MS Mus musculus 202 SH3KBP1 30011 Affinity Capture-MS Homo sapiens 203 VPS33B 26276 Cross-Linking-MS (XL-MS) Homo sapiens 204 SP7   Affinity Capture-MS Homo sapiens 205 PPP2R2D 55844 Affinity Capture-MS Homo sapiens 206 C9orf72   Affinity Capture-MS Homo sapiens View the network

Pathways in which ATAD3A is involved

No pathways found