|
 Gene ontology annotations for SRSF5 |
|
|
| Experiment description of studies that identified SRSF5 in exosomes |
| 1 |
| Experiment ID |
489 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 2 |
| Experiment ID |
490 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 3 |
| Experiment ID |
491 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 8 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 4 |
| Experiment ID |
492 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 9 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 5 |
| Experiment ID |
542 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299-fraction 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 6 |
| Experiment ID |
543 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299-fraction 4 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 7 |
| Experiment ID |
544 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299-fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 8 |
| Experiment ID |
545 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299-fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 9 |
| Experiment ID |
546 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|GAPDH
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 10 |
| Experiment ID |
548 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 11 |
| Experiment ID |
549 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 12 |
| Experiment ID |
550 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 13 |
| Experiment ID |
551 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 14 |
| Experiment ID |
554 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 15 |
| Experiment ID |
555 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 16 |
| Experiment ID |
556 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 17 |
| Experiment ID |
557 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975-fraction 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 18 |
| Experiment ID |
558 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975-fraction 4 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 19 |
| Experiment ID |
559 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975-fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 20 |
| Experiment ID |
560 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 21 |
| Experiment ID |
561 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 22 |
| Experiment ID |
562 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 23 |
| Experiment ID |
563 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 24 |
| Experiment ID |
564 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 25 |
| Experiment ID |
565 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 26 |
| Experiment ID |
566 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 27 |
| Experiment ID |
567 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 28 |
| Experiment ID |
568 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 29 |
| Experiment ID |
254 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
MNT-1 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 30 |
| Experiment ID |
259 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
A375M |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| Protein-protein interactions for SRSF5 |
| |
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
| 1 |
RPSAP58 |
388524 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 2 |
ZNF771 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 3 |
ZBTB11 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 4 |
ZNF346 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 5 |
SRPK2 |
6733 |
| Biochemical Activity |
|
Homo sapiens |
| Biochemical Activity |
|
Homo sapiens |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 6 |
MRPL24 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 7 |
SEPT2 |
4735 |
| Co-fractionation |
|
Homo sapiens |
|
| 8 |
OTUD3 |
23252 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 9 |
APEX1 |
328 |
| Affinity Capture-RNA |
|
Homo sapiens |
|
| 10 |
CENPU |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 11 |
RBM28 |
55131 |
| Affinity Capture-MS |
|
Homo sapiens |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 12 |
KIF23 |
9493 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 13 |
USP39 |
10713 |
| Co-fractionation |
|
Homo sapiens |
|
| 14 |
PPP3CB |
|
| Co-fractionation |
|
Homo sapiens |
|
| 15 |
AI837181 |
|
| Affinity Capture-MS |
|
Mus musculus |
|
| 16 |
FLII |
2314 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 17 |
MARCH5 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 18 |
RPS9 |
6203 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 19 |
RPF2 |
84154 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 20 |
PTCD1 |
26024 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 21 |
CENPQ |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 22 |
MECP2 |
4204 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 23 |
ARIH2 |
10425 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 24 |
SREK1 |
140890 |
| Affinity Capture-MS |
|
Homo sapiens |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 25 |
RPL19 |
6143 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 26 |
KIF14 |
9928 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 27 |
CHMP4C |
92421 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 28 |
UTP14A |
10813 |
| Co-fractionation |
|
Homo sapiens |
|
| 29 |
LIN28B |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 30 |
MEPCE |
56257 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 31 |
RBMX2 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 32 |
HIST1H2AG |
8969 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 33 |
SRSF3 |
6428 |
| Affinity Capture-MS |
|
Homo sapiens |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 34 |
YBX2 |
51087 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 35 |
PURG |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 36 |
HIST1H2AM |
8336 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 37 |
RPL18A |
6142 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 38 |
MYH9 |
4627 |
| Co-fractionation |
|
Homo sapiens |
|
| 39 |
RPL31 |
6160 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 40 |
CBX6 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 41 |
HIST1H1C |
3006 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 42 |
HECTD1 |
25831 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 43 |
SON |
6651 |
| Co-fractionation |
|
Homo sapiens |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 44 |
CDX1 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 45 |
GTPBP4 |
23560 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 46 |
ANLN |
54443 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 47 |
ABT1 |
29777 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 48 |
DAP3 |
7818 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 49 |
DOCK8 |
81704 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 50 |
KIF20A |
10112 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 51 |
SIRT7 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 52 |
SRSF4 |
6429 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 53 |
MRPL16 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 54 |
NPM3 |
10360 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 55 |
CUL3 |
8452 |
| Affinity Capture-MS |
|
Homo sapiens |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 56 |
EBNA1BP2 |
10969 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 57 |
PAPD5 |
64282 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 58 |
GADD45GIP1 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 59 |
RPL7L1 |
285855 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 60 |
DHX37 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 61 |
NIP7 |
51388 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 62 |
ZNF512 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 63 |
FASN |
2194 |
| Positive Genetic |
|
Homo sapiens |
| Negative Genetic |
|
Homo sapiens |
|
| 64 |
CMSS1 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 65 |
TMCO1 |
54499 |
| Co-fractionation |
|
Homo sapiens |
|
| 66 |
FBLL1 |
345630 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 67 |
HIST1H1A |
3024 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 68 |
ZFP62 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 69 |
TMX2 |
51075 |
| Co-fractionation |
|
Homo sapiens |
|
| 70 |
RPS16 |
6217 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 71 |
U2SURP |
23350 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 72 |
YBX1 |
4904 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 73 |
PRDM10 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 74 |
RRS1 |
23212 |
| Affinity Capture-MS |
|
Homo sapiens |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 75 |
MRPS18C |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 76 |
SMN2 |
6607 |
| Co-fractionation |
|
Homo sapiens |
|
| 77 |
TRMT112 |
| | |