Gene description for HADHB
Gene name hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit
Gene symbol HADHB
Other names/aliases ECHB
MSTP029
MTPB
TP-BETA
Species Homo sapiens
 Database cross references - HADHB
ExoCarta ExoCarta_3032
Entrez Gene 3032
HGNC 4803
MIM 143450
UniProt P55084  
 HADHB identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for HADHB
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    long-chain-3-hydroxyacyl-CoA dehydrogenase activity GO:0016509 IEA
    long-chain-enoyl-CoA hydratase activity GO:0016508 IEA
    protein complex binding GO:0032403 IEA
    acetyl-CoA C-acyltransferase activity GO:0003988 IEA
    protein binding GO:0005515 IPI
    NAD binding GO:0051287 IEA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 TAS
    fatty-acyl-CoA binding GO:0000062 IEA
    enoyl-CoA hydratase activity GO:0004300 IEA
Biological Process
    fatty acid beta-oxidation GO:0006635 TAS
    cardiolipin acyl-chain remodeling GO:0035965 TAS
    glycerophospholipid biosynthetic process GO:0046474 TAS
    cellular lipid metabolic process GO:0044255 TAS
    small molecule metabolic process GO:0044281 TAS
    phospholipid metabolic process GO:0006644 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial envelope GO:0005740 TAS
    endoplasmic reticulum GO:0005783 IDA
    mitochondrial nucleoid GO:0042645 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial fatty acid beta-oxidation multienzyme complex GO:0016507 IEA
    mitochondrion GO:0005739 NAS
 Experiment description of studies that identified HADHB in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HADHB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 VCL 7414
Affinity Capture-MS Homo sapiens
3 TNNI3K  
Two-hybrid Homo sapiens
View the network image/svg+xml



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