Gene description for KIF14
Gene name kinesin family member 14
Gene symbol KIF14
Other names/aliases MKS12
Species Homo sapiens
 Database cross references - KIF14
ExoCarta ExoCarta_9928
Vesiclepedia VP_9928
Entrez Gene 9928
HGNC 19181
MIM 611279
UniProt Q15058  
 KIF14 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for KIF14
Molecular Function
    microtubule motor activity GO:0003777 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISS
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 ISS
    plus-end-directed microtubule motor activity GO:0008574 ISS
    tubulin binding GO:0015631 ISS
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 ISS
    protein kinase binding GO:0019901 IPI
    PDZ domain binding GO:0030165 IDA
Biological Process
    regulation of cell growth GO:0001558 IMP
    microtubule-based movement GO:0007018 IBA
    microtubule depolymerization GO:0007019 ISS
    mitotic metaphase chromosome alignment GO:0007080 IMP
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IMP
    regulation of G2/M transition of mitotic cell cycle GO:0010389 IMP
    cerebellar granular layer structural organization GO:0021685 ISS
    cerebellar Purkinje cell layer structural organization GO:0021693 ISS
    cerebellar cortex development GO:0021695 ISS
    hippocampus development GO:0021766 ISS
    olfactory bulb development GO:0021772 ISS
    cell proliferation in forebrain GO:0021846 ISS
    cerebral cortex development GO:0021987 ISS
    regulation of cell adhesion GO:0030155 IMP
    regulation of cell migration GO:0030334 IMP
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IMP
    regulation of myelination GO:0031641 ISS
    activation of protein kinase activity GO:0032147 IMP
    positive regulation of cytokinesis GO:0032467 IMP
    regulation of Rap protein signal transduction GO:0032487 IMP
    negative regulation of integrin activation GO:0033624 IMP
    substrate adhesion-dependent cell spreading GO:0034446 IDA
    negative regulation of apoptotic process GO:0043066 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    regulation of neuron apoptotic process GO:0043523 ISS
    negative regulation of neuron apoptotic process GO:0043524 ISS
    establishment of protein localization GO:0045184 IDA
    cell division GO:0051301 IEA
    regulation of cell maturation GO:1903429 IEA
    regulation of G1/S transition of mitotic cell cycle GO:2000045 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IDA
    plasma membrane GO:0005886 IPI
    membrane GO:0016020 HDA
    midbody GO:0030496 IDA
    spindle midzone GO:0051233 IDA
    Flemming body GO:0090543 IDA
 Experiment description of studies that identified KIF14 in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIF14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFAP20 29105
Affinity Capture-MS Homo sapiens
2 ANKDD1B  
Affinity Capture-MS Homo sapiens
3 EIF3A 8661
Affinity Capture-MS Homo sapiens
4 RALYL 138046
Affinity Capture-MS Homo sapiens
5 OXCT1 5019
Affinity Capture-MS Homo sapiens
6 ITIH4 3700
Affinity Capture-MS Homo sapiens
7 SEC16A 9919
Affinity Capture-MS Homo sapiens
8 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
9 TOP2B 7155
Affinity Capture-MS Homo sapiens
10 GLB1 2720
Affinity Capture-MS Homo sapiens
11 CAP1 10487
Affinity Capture-MS Homo sapiens
12 RPL12 6136
Affinity Capture-MS Homo sapiens
13 TAF15 8148
Affinity Capture-MS Homo sapiens
14 GCN1L1 10985
Affinity Capture-MS Homo sapiens
15 AMOTL1 154810
Affinity Capture-MS Homo sapiens
16 DARS 1615
Affinity Capture-MS Homo sapiens
17 COL5A1 1289
Affinity Capture-MS Homo sapiens
18 CTDSPL2  
Affinity Capture-MS Homo sapiens
19 ACTR3 10096
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TRIOBP 11078
Affinity Capture-MS Homo sapiens
21 ERC1 23085
Affinity Capture-MS Homo sapiens
22 ABCD3 5825
Affinity Capture-MS Homo sapiens
23 RPS9 6203
Affinity Capture-MS Homo sapiens
24 SOGA3  
Affinity Capture-MS Homo sapiens
25 ECHDC3 79746
Affinity Capture-MS Homo sapiens
26 PPP6R3 55291
Affinity Capture-MS Homo sapiens
27 MRPL47 57129
Affinity Capture-MS Homo sapiens
28 TFRC 7037
Affinity Capture-MS Homo sapiens
29 FAM120A 23196
Affinity Capture-MS Homo sapiens
30 KIF14 9928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PDPN  
Affinity Capture-MS Homo sapiens
32 RBMX 27316
Affinity Capture-MS Homo sapiens
33 OSGEPL1  
Affinity Capture-MS Homo sapiens
34 TCP1 6950
Affinity Capture-MS Homo sapiens
35 UTP14A 10813
Affinity Capture-MS Homo sapiens
36 ACADVL 37
Affinity Capture-MS Homo sapiens
37 RRP9 9136
Affinity Capture-MS Homo sapiens
38 VRK1 7443
Affinity Capture-MS Homo sapiens
39 RBM4B  
Affinity Capture-MS Homo sapiens
40 PLD1 5337
Affinity Capture-MS Homo sapiens
41 TUFM 7284
Affinity Capture-MS Homo sapiens
42 OFD1 8481
Affinity Capture-MS Homo sapiens
43 NEDD4L 23327
Affinity Capture-MS Homo sapiens
44 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
45 ENO1 2023
Affinity Capture-MS Homo sapiens
46 TUBA1C 84790
Affinity Capture-MS Homo sapiens
47 PCDH8  
Affinity Capture-MS Homo sapiens
48 SHCBP1 79801
Affinity Capture-MS Homo sapiens
49 TPM3 7170
Affinity Capture-MS Homo sapiens
50 GTPBP4 23560
Affinity Capture-MS Homo sapiens
51 IQGAP2 10788
Affinity Capture-MS Homo sapiens
52 LRCH3 84859
Affinity Capture-MS Homo sapiens
53 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
54 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 TRIP13 9319
Affinity Capture-MS Homo sapiens
56 CISD1 55847
Affinity Capture-MS Homo sapiens
57 HADHA 3030
Affinity Capture-MS Homo sapiens
58 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 BTNL9  
Affinity Capture-MS Homo sapiens
60 MICAL2 9645
Affinity Capture-MS Homo sapiens
61 LMNB1 4001
Affinity Capture-MS Homo sapiens
62 SIRT7  
Affinity Capture-MS Homo sapiens
63 LMO7 4008
Affinity Capture-MS Homo sapiens
64 ACAD11 84129
Affinity Capture-MS Homo sapiens
65 CD274 29126
Affinity Capture-MS Homo sapiens
66 NEBL 10529
Affinity Capture-MS Homo sapiens
67 ATAD3C 219293
Affinity Capture-MS Homo sapiens
68 EPRS 2058
Affinity Capture-MS Homo sapiens
69 DHX37  
Affinity Capture-MS Homo sapiens
70 ZC3H11A  
Affinity Capture-MS Homo sapiens
71 NIP7 51388
Affinity Capture-MS Homo sapiens
72 STRAP 11171
Affinity Capture-MS Homo sapiens
73 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
74 TJP2 9414
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 KIF1BP 26128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 AHR 196
Affinity Capture-MS Homo sapiens
77 GSTK1 373156
Affinity Capture-MS Homo sapiens
78 N4BP3  
Affinity Capture-MS Homo sapiens
79 HPSE 10855
Affinity Capture-MS Homo sapiens
80 EFNB2 1948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 MAP3K7CL  
Affinity Capture-MS Homo sapiens
82 NADK2 133686
Affinity Capture-MS Homo sapiens
83 STX11 8676
Affinity Capture-MS Homo sapiens
84 HK2 3099
Affinity Capture-MS Homo sapiens
85 DDX46 9879
Affinity Capture-MS Homo sapiens
86 YBX1 4904
Affinity Capture-MS Homo sapiens
87 MCTP2  
Affinity Capture-MS Homo sapiens
88 TUBGCP2 10844
Protein-peptide Homo sapiens
89 CSNK1A1L 122011
Affinity Capture-MS Homo sapiens
90 RPL6 6128
Affinity Capture-MS Homo sapiens
91 MACF1 23499
Affinity Capture-MS Homo sapiens
92 MTA1 9112
Affinity Capture-MS Homo sapiens
93 ALPPL2 251
Affinity Capture-MS Homo sapiens
94 RSPRY1 89970
Affinity Capture-MS Homo sapiens
95 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
96 CYTH1 9267
Affinity Capture-MS Homo sapiens
97 TBL3 10607
Affinity Capture-MS Homo sapiens
98 TRA2B 6434
Affinity Capture-MS Homo sapiens
99 SSBP1 6742
Affinity Capture-MS Homo sapiens
100 PSMA1 5682
Affinity Capture-MS Homo sapiens
101 FAR1 84188
Affinity Capture-MS Homo sapiens
102 LARP7 51574
Affinity Capture-MS Homo sapiens
103 HNRNPLL 92906
Affinity Capture-MS Homo sapiens
104 DAPK3 1613
Affinity Capture-MS Homo sapiens
105 CEP135  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 CLTA 1211
Affinity Capture-MS Homo sapiens
107 NRIP1 8204
Affinity Capture-MS Homo sapiens
108 TMED7 51014
Affinity Capture-MS Homo sapiens
109 RPL23A 6147
Affinity Capture-MS Homo sapiens
110 NFIC 4782
Affinity Capture-MS Homo sapiens
111 NUP85 79902
Affinity Capture-MS Homo sapiens
112 CLMP 79827
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 ZSWIM7  
Affinity Capture-MS Homo sapiens
114 CTNND1 1500
Affinity Capture-MS Homo sapiens
115 CENPB 1059
Affinity Capture-MS Homo sapiens
116 MCM3 4172
Affinity Capture-MS Homo sapiens
117 USP6NL 9712
Affinity Capture-MS Homo sapiens
118 CPM 1368
Affinity Capture-MS Homo sapiens
119 TFB2M  
Affinity Capture-MS Homo sapiens
120 PRPF38A 84950
Affinity Capture-MS Homo sapiens
121 NACA 4666
Affinity Capture-MS Homo sapiens
122 URB1 9875
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 ARF3 377
Affinity Capture-MS Homo sapiens
124 RTN4RL2  
Affinity Capture-MS Homo sapiens
125 DIEXF  
Affinity Capture-MS Homo sapiens
126 SNRPF 6636
Affinity Capture-MS Homo sapiens
127 NDUFS2 4720
Affinity Capture-MS Homo sapiens
128 UAP1 6675
Affinity Capture-MS Homo sapiens
129 RPS18 6222
Affinity Capture-MS Homo sapiens
130 POTEE 445582
Affinity Capture-MS Homo sapiens
131 TNRC6C  
Affinity Capture-MS Homo sapiens
132 CORO1B 57175
Affinity Capture-MS Homo sapiens
133 DDX24 57062
Affinity Capture-MS Homo sapiens
134 SPIN1  
Affinity Capture-MS Homo sapiens
135 CCDC14  
Affinity Capture-MS Homo sapiens
136 MAGOHB 55110
Affinity Capture-MS Homo sapiens
137 KHDRBS1 10657
Affinity Capture-MS Homo sapiens
138 NUP93 9688
Affinity Capture-MS Homo sapiens
139 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 HELZ2  
Affinity Capture-MS Homo sapiens
141 SPDEF  
Affinity Capture-MS Homo sapiens
142 LACTB 114294
Affinity Capture-MS Homo sapiens
143 TPRN 286262
Affinity Capture-MS Homo sapiens
144 FGD6  
Affinity Capture-MS Homo sapiens
145 PSD2  
Affinity Capture-MS Homo sapiens
146 FTSJ1 24140
Affinity Capture-MS Homo sapiens
147 AMOT  
Proximity Label-MS Homo sapiens
148 RPL10L 140801
Affinity Capture-MS Homo sapiens
149 PES1 23481
Affinity Capture-MS Homo sapiens
150 YWHAB 7529
Affinity Capture-MS Homo sapiens
151 COBL  
Affinity Capture-MS Homo sapiens
152 ADAMTSL4  
Affinity Capture-MS Homo sapiens
153 DSG1 1828
Affinity Capture-MS Homo sapiens
154 CEP55 55165
Affinity Capture-MS Homo sapiens
155 MRPL45 84311
Affinity Capture-MS Homo sapiens
156 RPL4 6124
Affinity Capture-MS Homo sapiens
157 TRA2A 29896
Affinity Capture-MS Homo sapiens
158 GNAI1 2770
Affinity Capture-MS Homo sapiens
159 LYN 4067
Affinity Capture-MS Homo sapiens
160 CGN  
Affinity Capture-MS Homo sapiens
161 NKRF 55922
Affinity Capture-MS Homo sapiens
162 POLDIP2 26073
Affinity Capture-MS Homo sapiens
163 RPLP0 6175
Affinity Capture-MS Homo sapiens
164 RPLP0P6 220717
Affinity Capture-MS Homo sapiens
165 NTN3  
Affinity Capture-MS Homo sapiens
166 TPX2  
Affinity Capture-MS Homo sapiens
167 STON2 85439
Affinity Capture-MS Homo sapiens
168 CD40 958
Affinity Capture-MS Homo sapiens
169 MAP7 9053
Affinity Capture-MS Homo sapiens
170 ACLY 47
Affinity Capture-MS Homo sapiens
171 PAN3 255967
Affinity Capture-MS Homo sapiens
172 UGGT1 56886
Affinity Capture-MS Homo sapiens
173 EIF6 3692
Affinity Capture-MS Homo sapiens
174 TUBB4B 10383
Affinity Capture-MS Homo sapiens
175 YLPM1 56252
Affinity Capture-MS Homo sapiens
176 SPECC1 92521
Affinity Capture-MS Homo sapiens
177 TNRC6B  
Affinity Capture-MS Homo sapiens
178 LARS 51520
Affinity Capture-MS Homo sapiens
179 HAUS5 23354
Affinity Capture-MS Homo sapiens
180 SLC3A2 6520
Affinity Capture-MS Homo sapiens
181 TRAPPC4 51399
Affinity Capture-MS Homo sapiens
182 NPIPB6  
Affinity Capture-MS Homo sapiens
183 DDB1 1642
Affinity Capture-MS Homo sapiens
184 DDRGK1 65992
Affinity Capture-MS Homo sapiens
185 RHOB 388
Proximity Label-MS Homo sapiens
186 ARGLU1  
Affinity Capture-MS Homo sapiens
187 LARP4 113251
Affinity Capture-MS Homo sapiens
188 TRIM4 89122
Affinity Capture-MS Homo sapiens
189 PHGDH 26227
Affinity Capture-MS Homo sapiens
190 DSP 1832
Affinity Capture-MS Homo sapiens
191 PA2G4 5036
Affinity Capture-MS Homo sapiens
192 SLC2A12 154091
Affinity Capture-MS Homo sapiens
193 SUN1 23353
Affinity Capture-MS Homo sapiens
194 ILF3 3609
Affinity Capture-MS Homo sapiens
195 DPYSL5  
Affinity Capture-MS Homo sapiens
196 TEX28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
197 VIM 7431
Affinity Capture-MS Homo sapiens
198 FXR1 8087
Affinity Capture-MS Homo sapiens
199 SF3B4 10262
Affinity Capture-MS Homo sapiens
200 KEAP1 9817
Affinity Capture-MS Homo sapiens
201 SIPA1L3  
Affinity Capture-MS Homo sapiens
202 HLTF  
Affinity Capture-MS Homo sapiens
203 KNOP1 400506
Affinity Capture-MS Homo sapiens
204 RPS4X 6191
Affinity Capture-MS Homo sapiens
205 SQSTM1 8878
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
206 TROAP  
Affinity Capture-MS Homo sapiens
207 CENPF 1063
Affinity Capture-MS Homo sapiens
208 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
209 AGO2 27161
Affinity Capture-MS Homo sapiens
210 BUB3 9184
Affinity Capture-MS Homo sapiens
211 CDK1 983
Affinity Capture-MS Homo sapiens
212 YTHDF3  
Affinity Capture-MS Homo sapiens
213 RPS26 6231
Affinity Capture-MS Homo sapiens
214 NLN 57486
Affinity Capture-MS Homo sapiens
215 IDH2 3418
Affinity Capture-MS Homo sapiens
216 OSBPL3 26031
Affinity Capture-MS Homo sapiens
217 RRAS 6237
Affinity Capture-MS Homo sapiens
218 YBX3 8531
Affinity Capture-MS Homo sapiens
219 ATP1A1 476
Affinity Capture-MS Homo sapiens
220 ALPP 250
Affinity Capture-MS Homo sapiens
221 TNNC2  
Affinity Capture-MS Homo sapiens
222 C1QBP 708
Affinity Capture-MS Homo sapiens
223 MTA3 57504
Affinity Capture-MS Homo sapiens
224 HSPA4L 22824
Affinity Capture-MS Homo sapiens
225 CKAP4 10970
Affinity Capture-MS Homo sapiens
226 RALGAPA2  
Affinity Capture-MS Homo sapiens
227 CUL4A 8451
Affinity Capture-MS Homo sapiens
228 AP2S1 1175
Affinity Capture-MS Homo sapiens
229 SLIRP 81892
Affinity Capture-MS Homo sapiens
230 SENP3 26168
Affinity Capture-MS Homo sapiens
231