Experiment description of studies that identified RPLP0P6 in sEVs

1 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

2 Experiment ID 1275 MISEV standards Biophysical techniques ✔ CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 1280 MISEV standards Biophysical techniques ✔ TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.151 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

Protein-protein interactions for RPLP0P6

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RPSA 3921 Co-fractionation Homo sapiens 2 NOP58 51602 Co-fractionation Homo sapiens 3 RPS28 6234 Co-fractionation Homo sapiens 4 RPS3A 6189 Co-fractionation Homo sapiens 5 RPL30 6156 Co-fractionation Homo sapiens 6 RPS17 6218 Co-fractionation Homo sapiens 7 RPL37A 6168 Co-fractionation Homo sapiens 8 RPS19 6223 Co-fractionation Homo sapiens 9 RPL10 6134 Co-fractionation Homo sapiens 10 RPL15 6138 Co-fractionation Homo sapiens 11 HSP90AB1 3326 Co-fractionation Homo sapiens 12 EIF4A1 1973 Co-fractionation Homo sapiens 13 RPL12 6136 Co-fractionation Homo sapiens 14 RPS3 6188 Co-fractionation Homo sapiens 15 ILF2 3608 Co-fractionation Homo sapiens 16 HIST2H2AA3 8337 Cross-Linking-MS (XL-MS) Homo sapiens 17 EFNA3   Affinity Capture-MS Homo sapiens 18 RPS20 6224 Co-fractionation Homo sapiens 19 PRC1 9055 Affinity Capture-MS Homo sapiens 20 KIF23 9493 Affinity Capture-MS Homo sapiens 21 MCM2 4171 Affinity Capture-MS Homo sapiens 22 EIF3B 8662 Co-fractionation Homo sapiens 23 IMPDH2 3615 Co-fractionation Homo sapiens 24 HSPH1 10808 Co-fractionation Homo sapiens 25 RPS8 6202 Co-fractionation Homo sapiens 26 RPL29 6159 Co-fractionation Homo sapiens 27 TOMM40 10452 Co-fractionation Homo sapiens 28 TFCP2 7024 Affinity Capture-MS Homo sapiens 29 RPS11 6205 Co-fractionation Homo sapiens 30 RPL19 6143 Co-fractionation Homo sapiens 31 KIF14 9928 Affinity Capture-MS Homo sapiens 32 RPL10L 140801 Co-fractionation Homo sapiens 33 CHMP4C 92421 Affinity Capture-MS Homo sapiens 34 RPS23 6228 Co-fractionation Homo sapiens 35 RPL27 6155 Co-fractionation Homo sapiens 36 RPL9 6133 Co-fractionation Homo sapiens 37 RPL4 6124 Co-fractionation Homo sapiens 38 RPS10 6204 Co-fractionation Homo sapiens 39 TUBA1A 7846 Co-fractionation Homo sapiens 40 NOP2 4839 Co-fractionation Homo sapiens 41 RPL5 6125 Co-fractionation Homo sapiens 42 RPL18A 6142 Co-fractionation Homo sapiens 43 CHMP4B 128866 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens 44 YAP1 10413 Affinity Capture-MS Homo sapiens 45 RPL31 6160 Co-fractionation Homo sapiens 46 LUC7L2 51631 Co-fractionation Homo sapiens 47 RPLP1 6176 Co-fractionation Homo sapiens 48 TUBA1B 10376 Co-fractionation Homo sapiens 49 RPS6 6194 Co-fractionation Homo sapiens 50 RPLP0 6175 Co-fractionation Homo sapiens 51 RPS15A 6210 Co-fractionation Homo sapiens 52 U2AF1 7307 Co-fractionation Homo sapiens 53 RPL11 6135 Co-fractionation Homo sapiens 54 MAT2A 4144 Co-fractionation Homo sapiens 55 ANLN 54443 Affinity Capture-MS Homo sapiens 56 HIST1H2AD 3013 Co-fractionation Homo sapiens 57 RPL32 6161 Co-fractionation Homo sapiens 58 EFNA4   Affinity Capture-MS Homo sapiens 59 HADHB 3032 Co-fractionation Homo sapiens 60 RPLP2 6181 Co-fractionation Homo sapiens 61 RPL7A 6130 Co-fractionation Homo sapiens 62 RPS2 6187 Co-fractionation Homo sapiens 63 ECT2 1894 Affinity Capture-MS Homo sapiens 64 EEF2 1938 Co-fractionation Homo sapiens 65 RPL14 9045 Co-fractionation Homo sapiens 66 RPS7 6201 Co-fractionation Homo sapiens 67 TOMM22 56993 Co-fractionation Homo sapiens 68 RPL21 6144 Co-fractionation Homo sapiens 69 RPL3 6122 Co-fractionation Homo sapiens 70 RPL24 6152 Co-fractionation Homo sapiens 71 EEF1B2 1933 Co-fractionation Homo sapiens 72 RPL7 6129 Co-fractionation Homo sapiens 73 RIPK4   Affinity Capture-MS Homo sapiens 74 DKC1 1736 Co-fractionation Homo sapiens 75 RPS13 6207 Co-fractionation Homo sapiens 76 RPS14 6208 Co-fractionation Homo sapiens 77 NHP2L1 4809 Co-fractionation Homo sapiens 78 RPL22 6146 Co-fractionation Homo sapiens 79 RPL18 6141 Co-fractionation Homo sapiens 80 VDAC1 7416 Co-fractionation Homo sapiens 81 GNL3 26354 Co-fractionation Homo sapiens 82 RPS16 6217 Co-fractionation Homo sapiens 83 EEF1A1 1915 Co-fractionation Homo sapiens 84 RPL13 6137 Co-fractionation Homo sapiens 85 RPS27A 6233 Co-fractionation Homo sapiens 86 RPS25 6230 Co-fractionation Homo sapiens 87 SLC25A6 293 Co-fractionation Homo sapiens 88 ILF3 3609 Co-fractionation Homo sapiens 89 RPL6 6128 Co-fractionation Homo sapiens 90 RPS21 6227 Co-fractionation Homo sapiens 91 RPS12 6206 Co-fractionation Homo sapiens 92 HNRNPM 4670 Co-fractionation Homo sapiens 93 RPS4X 6191 Co-fractionation Homo sapiens 94 RPS10P5 93144 Co-fractionation Homo sapiens 95 RPS5 6193 Co-fractionation Homo sapiens 96 TARS 6897 Co-fractionation Homo sapiens 97 RPS26 6231 Co-fractionation Homo sapiens 98 RPL10A 4736 Co-fractionation Homo sapiens 99 RPS24 6229 Co-fractionation Homo sapiens 100 MOV10 4343 Co-fractionation Homo sapiens 101 SLC25A4 291 Co-fractionation Homo sapiens 102 RPL23 9349 Co-fractionation Homo sapiens 103 CIT 11113 Affinity Capture-MS Homo sapiens 104 RPL8 6132 Co-fractionation Homo sapiens 105 RPS27L 51065 Co-fractionation Homo sapiens 106 SERBP1 26135 Affinity Capture-MS Homo sapiens 107 RPL23A 6147 Co-fractionation Homo sapiens 108 C9orf72   Affinity Capture-MS Homo sapiens 109 AR 367 Affinity Capture-MS Homo sapiens 110 VDAC3 7419 Co-fractionation Homo sapiens 111 RPL17 6139 Co-fractionation Homo sapiens View the network

Pathways in which RPLP0P6 is involved

No pathways found