Gene description for POTEKP
Gene name POTE ankyrin domain family, member K, pseudogene
Gene symbol POTEKP
Other names/aliases ACT
ACTBL3
POTE2delta
POTEK
Species Homo sapiens
 Database cross references - POTEKP
ExoCarta ExoCarta_440915
Vesiclepedia VP_440915
Entrez Gene 440915
HGNC 30182
MIM 611266
 POTEKP identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Plasma 19028452    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for POTEKP
Molecular Function
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IBA
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 IBA
Biological Process
    axonogenesis GO:0007409 IBA
    cell motility GO:0048870 IBA
    postsynaptic actin cytoskeleton organization GO:0098974 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    actin filament GO:0005884 IBA
    actin cytoskeleton GO:0015629 IBA
    membrane GO:0016020 IBA
    axon GO:0030424 IBA
    NuA4 histone acetyltransferase complex GO:0035267 IBA
    synapse GO:0045202 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified POTEKP in exosomes
1
Experiment ID 20
MISEV standards
EM
Biophysical techniques
HSP90|CD63|CD81|LAMP1
Enriched markers
GOLGA2|cytochrome c
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 21
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101|HSP70|CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 44
MISEV standards
IEM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for POTEKP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Co-fractionation Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 DST 667
Co-fractionation Homo sapiens
4 C14orf105  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 LGR4 55366
Affinity Capture-MS Homo sapiens
6 HMGN2 3151
Cross-Linking-MS (XL-MS) Homo sapiens
7 KIF14 9928
Affinity Capture-MS Homo sapiens
8 KIF20A 10112
Affinity Capture-MS Homo sapiens
9 SDHA 6389
Affinity Capture-MS Homo sapiens
10 ECT2 1894
Affinity Capture-MS Homo sapiens
11 COBL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ACTA2 59
Affinity Capture-MS Homo sapiens
13 GC 2638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 DBN1 1627
Affinity Capture-MS Homo sapiens
15 YWHAZ 7534
Co-fractionation Homo sapiens
16 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
17 PNMAL1  
Affinity Capture-MS Homo sapiens
18 FAM120AOS  
Affinity Capture-MS Homo sapiens
19 ERBB3 2065
Affinity Capture-MS Homo sapiens
20 OPRL1 4987
Affinity Capture-MS Homo sapiens
21 NAT6  
Affinity Capture-MS Homo sapiens
22 HSP90B1 7184
Cross-Linking-MS (XL-MS) Homo sapiens
23 TFCP2 7024
Affinity Capture-MS Homo sapiens
24 BACH1 571
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 AURKB 9212
Affinity Capture-MS Homo sapiens
26 CDYL 9425
Affinity Capture-MS Homo sapiens
27 CHMP4B 128866
Affinity Capture-MS Homo sapiens
28 YAP1 10413
Affinity Capture-MS Homo sapiens
29 ERRFI1 54206
Affinity Capture-MS Homo sapiens
30 KIF23 9493
Affinity Capture-MS Homo sapiens
31 DAZL  
Affinity Capture-MS Homo sapiens
32 CYB5R3 1727
Co-fractionation Homo sapiens
33 SCML1  
Affinity Capture-MS Homo sapiens
34 PRC1 9055
Affinity Capture-MS Homo sapiens
35 ACTB 60
Affinity Capture-MS Homo sapiens
36 LOC100288778  
Affinity Capture-MS Homo sapiens
37 BTK 695
Affinity Capture-MS Homo sapiens
38 WASH1 100287171
Affinity Capture-MS Homo sapiens
39 TXNDC8 255220
Affinity Capture-MS Homo sapiens
40 ARMC10 83787
Affinity Capture-MS Homo sapiens
41 MFF 56947
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PDGFRA 5156
Affinity Capture-MS Homo sapiens
43 RNF217  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 RAB23 51715
Affinity Capture-MS Homo sapiens
45 UCHL5 51377
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which POTEKP is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here