Experiment description of studies that identified LYAR in exosomes

1 Experiment ID 207 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 ISEV standards ✘ EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 257 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name Daju Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 126 ISEV standards ✘ EV Biophysical techniques ✔ GAPDH EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

Protein-protein interactions for LYAR

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GNL2 29889 Affinity Capture-MS Homo sapiens 2 TWF2 11344 Affinity Capture-MS Homo sapiens 3 DHX57   Affinity Capture-MS Homo sapiens 4 RPS3A 6189 Affinity Capture-MS Homo sapiens 5 KPNA1 3836 Affinity Capture-MS Homo sapiens 6 ZHX1   Two-hybrid Homo sapiens 7 EIF3I 8668 Affinity Capture-MS Homo sapiens 8 BRIX1 55299 Affinity Capture-MS Homo sapiens 9 KRTHB2   Affinity Capture-MS Homo sapiens 10 NAT10 55226 Affinity Capture-MS Homo sapiens 11 DDX21 9188 Affinity Capture-MS Homo sapiens 12 RPL21 6144 Affinity Capture-MS Homo sapiens 13 RPL3 6122 Affinity Capture-MS Homo sapiens 14 EBNA1BP2 10969 Affinity Capture-MS Homo sapiens 15 ZBTB16   Two-hybrid Homo sapiens 16 RPS9   Affinity Capture-MS Homo sapiens 17 RPL18A 6142 Affinity Capture-MS Homo sapiens 18 RPL31 6160 Affinity Capture-MS Homo sapiens 19 RPL23A 6147 Affinity Capture-MS Homo sapiens 20 NPM1 4869 Affinity Capture-MS Homo sapiens 21 DHX9 1660 Affinity Capture-MS Homo sapiens 22 TSR1 55720 Affinity Capture-MS Homo sapiens 23 SRP14 6727 Affinity Capture-MS Homo sapiens 24 KRT31 3881 Affinity Capture-MS Homo sapiens 25 MYBBP1A 10514 Affinity Capture-MS Homo sapiens 26 DDX27 55661 Affinity Capture-MS Homo sapiens 27 GTPBP4 23560 Affinity Capture-MS Homo sapiens 28 KRTHB5   Affinity Capture-MS Homo sapiens View the network

Pathways in which LYAR is involved

No pathways found