Gene description for TPM1
Gene name tropomyosin 1 (alpha)
Gene symbol TPM1
Other names/aliases C15orf13
CMD1Y
CMH3
HTM-alpha
LVNC9
TMSA
Species Homo sapiens
 Database cross references - TPM1
ExoCarta ExoCarta_7168
Vesiclepedia VP_7168
Entrez Gene 7168
HGNC 12010
MIM 191010
UniProt P09493  
 TPM1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 17956143    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Keratinocytes 19530224    
Keratinocytes 19530224    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for TPM1
Molecular Function
    actin binding GO:0003779 TAS
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 IPI
    structural constituent of muscle GO:0008307 TAS
    identical protein binding GO:0042802 ISS
    protein homodimerization activity GO:0042803 ISS
    protein heterodimerization activity GO:0046982 ISS
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 ISS
Biological Process
    positive regulation of heart rate by epinephrine GO:0003065 ISS
    regulation of muscle contraction GO:0006937 TAS
    cytoskeleton organization GO:0007010 TAS
    actin filament organization GO:0007015 IBA
    regulation of heart contraction GO:0008016 TAS
    regulation of cell shape GO:0008360 IMP
    muscle filament sliding GO:0030049 ISS
    negative regulation of cell migration GO:0030336 ISS
    ruffle organization GO:0031529 ISS
    positive regulation of ATP-dependent activity GO:0032781 ISS
    cellular response to reactive oxygen species GO:0034614 IEP
    wound healing GO:0042060 ISS
    sarcomere organization GO:0045214 IMP
    positive regulation of cell adhesion GO:0045785 ISS
    positive regulation of stress fiber assembly GO:0051496 ISS
    ventricular cardiac muscle tissue morphogenesis GO:0055010 IMP
    cardiac muscle contraction GO:0060048 IBA
    cardiac muscle contraction GO:0060048 IMP
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 IMP
    negative regulation of vascular associated smooth muscle cell migration GO:1904753 IMP
Subcellular Localization
    stress fiber GO:0001725 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    muscle thin filament tropomyosin GO:0005862 TAS
    actin filament GO:0005884 IBA
    actin cytoskeleton GO:0015629 IDA
    sarcomere GO:0030017 TAS
    bleb GO:0032059 IMP
    ruffle membrane GO:0032587 IDA
 Experiment description of studies that identified TPM1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
14
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TPM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLFN11 91607
Proximity Label-MS Homo sapiens
2 APPL2 55198
Co-fractionation Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 PPP1CB 5500
Affinity Capture-MS Homo sapiens
5 BLOC1S6  
Two-hybrid Homo sapiens
6 STAT5B 6777
Affinity Capture-MS Homo sapiens
7 HDAC1 3065
Co-fractionation Homo sapiens
8 YEATS4  
Two-hybrid Homo sapiens
9 SYCE1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 COX4I1 1327
Co-fractionation Homo sapiens
11 TRIM26 7726
Affinity Capture-MS Homo sapiens
12 TPM2 7169
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
13 SP1  
Affinity Capture-MS Homo sapiens
14 Calml3  
Affinity Capture-MS Mus musculus
15 ACTC1 70
Co-fractionation Homo sapiens
16 DBN1 1627
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
17 MYOC 4653
Affinity Capture-MS Homo sapiens
18 JUND 3727
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 APOE 348
Affinity Capture-MS Homo sapiens
20 DLG5 9231
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 Actb 11461
Affinity Capture-MS Mus musculus
22 PARK7 11315
Affinity Capture-MS Homo sapiens
23 RPS3 6188
Co-fractionation Homo sapiens
24 TGFBR1 7046
Affinity Capture-Western Homo sapiens
25 APEX1 328
Affinity Capture-RNA Homo sapiens
26 NUDCD2 134492
Co-fractionation Homo sapiens
27 WTAP 9589
Affinity Capture-MS Homo sapiens
28 FBXW7  
Affinity Capture-MS Homo sapiens
29 MYH6 4624
Co-fractionation Homo sapiens
30 DYRK1A 1859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CFAP97  
Affinity Capture-MS Homo sapiens
32 SF3B1 23451
Co-fractionation Homo sapiens
33 EIF4A3 9775
Co-fractionation Homo sapiens
34 SPAG9 9043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 Poc1b  
Affinity Capture-MS Mus musculus
36 IQGAP1 8826
Affinity Capture-MS Homo sapiens
37 MDC1  
Affinity Capture-MS Homo sapiens
38 EAPP  
Affinity Capture-MS Homo sapiens
39 ETFA 2108
Co-fractionation Homo sapiens
40 CNN2 1265
Co-fractionation Homo sapiens
41 WASH1 100287171
Two-hybrid Homo sapiens
42 ELF3 1999
Affinity Capture-MS Homo sapiens
43 Myh9 17886
Affinity Capture-MS Mus musculus
44 BAG5 9529
Affinity Capture-MS Homo sapiens
45 DNAJC7 7266
Co-fractionation Homo sapiens
46 ACSL1 2180
Co-fractionation Homo sapiens
47 SEC23A 10484
Co-fractionation Homo sapiens
48 PPP6R3 55291
Co-fractionation Homo sapiens
49 TFCP2 7024
Affinity Capture-MS Homo sapiens
50 MYCN  
Affinity Capture-MS Homo sapiens
51 PSMA1 5682
Co-fractionation Homo sapiens
52 IFIT3 3437
Two-hybrid Homo sapiens
53 THOC1 9984
Co-fractionation Homo sapiens
54 Lima1  
Affinity Capture-MS Mus musculus
55 AHCYL1 10768
Co-fractionation Homo sapiens
56 Haus4  
Affinity Capture-MS Mus musculus
57 CCDC102A 92922
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 C1orf216  
Two-hybrid Homo sapiens
59 CHMP4C 92421
Affinity Capture-MS Homo sapiens
60 ACTA2 59
Co-fractionation Homo sapiens
61 TOLLIP 54472
Proximity Label-MS Homo sapiens
62 GATA4  
Affinity Capture-MS Homo sapiens
63 DLD 1738
Affinity Capture-MS Homo sapiens
64 MYO18A 399687
Affinity Capture-MS Homo sapiens
65 COX15 1355
Affinity Capture-MS Homo sapiens
66 NEB 4703
Reconstituted Complex Homo sapiens
67 CNTLN 54875
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PARK2  
Affinity Capture-MS Homo sapiens
69 FGA 2243
Two-hybrid Homo sapiens
70 HSP90B1 7184
Co-fractionation Homo sapiens
71 FAM9C  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
72 PSMD13 5719
Co-fractionation Homo sapiens
73 RUFY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 EXOC4 60412
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
76 ATF2  
Affinity Capture-MS Homo sapiens
77 RAP1B 5908
Affinity Capture-MS Homo sapiens
78 YAP1 10413
Affinity Capture-MS Homo sapiens
79 MYH9 4627
Affinity Capture-MS Homo sapiens
80 ACTN4 81
Co-fractionation Homo sapiens
81 OPA1 4976
Proximity Label-MS Homo sapiens
82 IKBIP 121457
Affinity Capture-MS Homo sapiens
83 YWHAQ 10971
Affinity Capture-MS Homo sapiens
84 H19 283120
Protein-RNA Homo sapiens
85 Coro1c 23790
Affinity Capture-MS Mus musculus
86 THOC2 57187
Co-fractionation Homo sapiens
87 PSMD2 5708
Co-fractionation Homo sapiens
88 CAPZB 832
Affinity Capture-MS Homo sapiens
89 DNM1L 10059
Affinity Capture-MS Homo sapiens
90 MYH4  
Co-fractionation Homo sapiens
91 BRCA1 672
Affinity Capture-MS Homo sapiens
92 LRCH3 84859
Affinity Capture-MS Homo sapiens
93 MYH2  
Co-fractionation Homo sapiens
94 ENDOG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 MYH7B  
Co-fractionation Homo sapiens
97 ACTN1 87
Co-fractionation Homo sapiens
98 SF3B2 10992
Co-fractionation Homo sapiens
99 GOLGA3 2802
Affinity Capture-MS Homo sapiens
100 PPP4R2  
Co-fractionation Homo sapiens
101 NKX2-5  
Affinity Capture-MS Homo sapiens
102 MYH8 4626
Co-fractionation Homo sapiens
103 NDUFB10 4716
Co-fractionation Homo sapiens
104 ECT2 1894
Affinity Capture-MS Homo sapiens
105 SRXN1 140809
Co-fractionation Homo sapiens
106 MAD1L1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 NELFCD 51497
Co-fractionation Homo sapiens
108 CTNNB1 1499
Affinity Capture-MS Homo sapiens
109 TNNT2  
Reconstituted Complex Homo sapiens
110 Ttll12 223723
Affinity Capture-MS Mus musculus
111 Itga5 16402
Affinity Capture-MS Mus musculus
112 ATP5F1 515
Co-fractionation Homo sapiens
113 BTF3 689
Affinity Capture-MS Homo sapiens
114 HMGB1 3146
Co-fractionation Homo sapiens
115 EGFR 1956
PCA Homo sapiens
116 MYO19  
Affinity Capture-MS Homo sapiens
117 TFPT  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
118 FOXL1  
Proximity Label-MS Homo sapiens
119 TSSC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 SORD 6652
Co-fractionation Homo sapiens
121 LARS 51520
Affinity Capture-MS Homo sapiens
122 SPTBN1 6711
Affinity Capture-Western Homo sapiens
123 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
124 ZYX 7791
Co-fractionation Homo sapiens
125 CDK2 1017
Affinity Capture-MS Homo sapiens
126 TPM1 7168
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
127 SOAT1 6646
Affinity Capture-MS Homo sapiens
128 SYNPO 11346
Affinity Capture-MS Homo sapiens
129 FOS 2353
Proximity Label-MS Homo sapiens
130 MYC  
Affinity Capture-MS Homo sapiens
131 Uso1 56041
Affinity Capture-MS Mus musculus
132 IRF8  
Affinity Capture-MS Homo sapiens
133 DLST 1743
Affinity Capture-MS Homo sapiens
134 Sesn2  
Affinity Capture-MS Mus musculus
135 EXOC7 23265
Affinity Capture-MS Homo sapiens
136 ACTN2 88
Co-fractionation Homo sapiens
137 CROCC 9696
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 VDAC1 7416
Affinity Capture-MS Homo sapiens
139 HOMER1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 2510003E04Rik  
Affinity Capture-MS Mus musculus
141 FOXK1 221937
Co-fractionation Homo sapiens
142 PSMB1 5689
Co-fractionation Homo sapiens
143 TPM4 7171
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
144 MGEA5 10724
Co-fractionation Homo sapiens
145 C10orf2 56652
Affinity Capture-MS Homo sapiens
146 PIN4 5303
Affinity Capture-MS Homo sapiens
147 TPM3 7170
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
148 TNNT1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
149 STUB1 10273
Co-fractionation Homo sapiens
150 NES 10763
Affinity Capture-MS Homo sapiens
151 TLX1  
Affinity Capture-MS Homo sapiens
152 PDLIM7 9260
Affinity Capture-MS Homo sapiens
153 RBPJ 3516
Affinity Capture-MS Homo sapiens
154 PSMB10 5699
Affinity Capture-MS Homo sapiens
155 Tmod3 50875
Affinity Capture-MS Mus musculus
156 Tmed10 68581
Affinity Capture-MS Mus musculus
157 KIAA1598 57698
Co-fractionation Homo sapiens
158 TMPO 7112
Affinity Capture-MS Homo sapiens
159 TP53 7157
Affinity Capture-MS Homo sapiens
160 CNN1 1264
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
161 CCHCR1  
Two-hybrid Homo sapiens
162 SULT1A1 6817
Co-fractionation Homo sapiens
163 CKB 1152
Co-fractionation Homo sapiens
164 Myo1c 17913
Affinity Capture-MS Mus musculus
165 CDC37 11140
Co-fractionation Homo sapiens
166 IKBKG 8517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
167 COX5A 9377
Co-fractionation Homo sapiens
168 CAPZA2 830
Affinity Capture-MS Homo sapiens
169 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
170 CAGE1 285782
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
171 RBM15 64783
Co-fractionation Homo sapiens
172 Sass6  
Affinity Capture-MS Mus musculus
173 MYH1  
Co-fractionation Homo sapiens
174 Myh10 77579
Affinity Capture-MS Mus musculus
175 UFL1 23376
Affinity Capture-MS Homo sapiens
176 Samm50  
Affinity Capture-MS Mus musculus
177 ABI2 10152
Co-fractionation Homo sapiens
178 ZNF746  
Affinity Capture-MS Homo sapiens
179 SCLT1 132320
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
180 Mapre1 13589
Affinity Capture-MS Mus musculus
181 1810055G02Rik  
Affinity Capture-MS Mus musculus
182 LRRK2 120892
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
183 FLNA 2316
Affinity Capture-MS Homo sapiens
184 KIF14 9928
Affinity Capture-MS Homo sapiens
185 ZIC3  
Affinity Capture-MS Homo sapiens
186 KXD1 79036
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
187 STAT1 6772
Proximity Label-MS Homo sapiens
188 ERG  
Affinity Capture-MS Homo sapiens
189 Shcbp1 20419
Affinity Capture-MS Mus musculus
190 AR 367
Affinity Capture-MS Homo sapiens
191 CCDC96  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
192 KLHL21 9903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
193 Tpm4  
Affinity Capture-MS Mus musculus
194 TNNI1 7135
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which TPM1 is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Smooth Muscle Contraction TAS Reactome
Striated Muscle Contraction TAS Reactome





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