Gene description for PRPF38B
Gene name pre-mRNA processing factor 38B
Gene symbol PRPF38B
Other names/aliases NET1
Species Homo sapiens
 Database cross references - PRPF38B
ExoCarta ExoCarta_55119
Vesiclepedia VP_55119
Entrez Gene 55119
HGNC 25512
UniProt Q5VTL8  
 PRPF38B identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PRPF38B
Molecular Function
    RNA binding GO:0003723 HDA
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
Subcellular Localization
    precatalytic spliceosome GO:0071011 IBA
 Experiment description of studies that identified PRPF38B in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRPF38B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 EIF3A 8661
Cross-Linking-MS (XL-MS) Homo sapiens
4 SERF2 10169
Affinity Capture-MS Homo sapiens
5 H2AFJ 55766
Affinity Capture-MS Homo sapiens
6 SRSF11 9295
Affinity Capture-MS Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
8 SRPK2 6733
Affinity Capture-MS Homo sapiens
9 RIT1 6016
Negative Genetic Homo sapiens
10 TACO1  
Affinity Capture-MS Homo sapiens
11 DGCR14  
Two-hybrid Homo sapiens
12 KNOP1 400506
Affinity Capture-MS Homo sapiens
13 UQCR11  
Affinity Capture-MS Homo sapiens
14 UBE2H 7328
Affinity Capture-MS Homo sapiens
15 EEF1G 1937
Co-fractionation Homo sapiens
16 CLK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 JPH3 57338
Affinity Capture-MS Homo sapiens
18 KIF2C 11004
Affinity Capture-MS Homo sapiens
19 PSENEN  
Affinity Capture-MS Homo sapiens
20 ECT2 1894
Affinity Capture-MS Homo sapiens
21 TCEAL7  
Affinity Capture-MS Homo sapiens
22 TBC1D9  
Affinity Capture-MS Homo sapiens
23 CD2BP2 10421
Affinity Capture-MS Homo sapiens
24 NF2 4771
Affinity Capture-MS Homo sapiens
25 CTNNB1 1499
Affinity Capture-MS Homo sapiens
26 ANAPC15  
Affinity Capture-MS Homo sapiens
27 SREBF1 6720
Negative Genetic Homo sapiens
28 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 TPX2  
Affinity Capture-MS Homo sapiens
30 IFI27L1  
Affinity Capture-MS Homo sapiens
31 TMEM14B  
Affinity Capture-MS Homo sapiens
32 WRAP53  
Affinity Capture-MS Homo sapiens
33 RPL35A 6165
Affinity Capture-MS Homo sapiens
34 ODF2 4957
Cross-Linking-MS (XL-MS) Homo sapiens
35 IFI6  
Affinity Capture-MS Homo sapiens
36 C9orf114  
Affinity Capture-MS Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 Bsdc1  
Affinity Capture-MS Mus musculus
39 EZH1  
Affinity Capture-MS Homo sapiens
40 SLTM 79811
Affinity Capture-MS Homo sapiens
41 LSM5 23658
Two-hybrid Homo sapiens
42 KRTAP19-7  
Affinity Capture-MS Homo sapiens
43 ANKRD50 57182
Affinity Capture-MS Homo sapiens
44 SRSF6 6431
Affinity Capture-MS Homo sapiens
45 UFL1 23376
Affinity Capture-MS Homo sapiens
46 C20orf197  
Affinity Capture-MS Homo sapiens
47 MYCN  
Affinity Capture-MS Homo sapiens
48 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
49 PTP4A3  
Affinity Capture-MS Homo sapiens
50 LRPAP1 4043
Affinity Capture-MS Homo sapiens
51 CEBPA  
Protein-peptide Homo sapiens
52 TMEM257  
Affinity Capture-MS Homo sapiens
53 SPCS1 28972
Affinity Capture-MS Homo sapiens
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 ZC3H18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 KIF23 9493
Affinity Capture-MS Homo sapiens
57 INO80B 83444
Affinity Capture-MS Homo sapiens
58 RAD18  
Affinity Capture-MS Homo sapiens
59 PRC1 9055
Affinity Capture-MS Homo sapiens
60 DPM2 8818
Affinity Capture-MS Homo sapiens
61 LYAR 55646
Affinity Capture-MS Homo sapiens
62 RBFOX2 23543
Two-hybrid Homo sapiens
63 CYCS 54205
Affinity Capture-MS Homo sapiens
64 DDRGK1 65992
Affinity Capture-MS Homo sapiens
65 YWHAG 7532
Affinity Capture-MS Homo sapiens
66 OBSL1 23363
Affinity Capture-MS Homo sapiens
67 SUMO1 7341
Affinity Capture-MS Homo sapiens
68 NANOG  
Affinity Capture-MS Homo sapiens
69 SAP18 10284
Affinity Capture-MS Homo sapiens
70 FOLR1 2348
Affinity Capture-MS Homo sapiens
71 LUC7L 55692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 PEA15 8682
Affinity Capture-MS Homo sapiens
73 KRAS 3845
Negative Genetic Homo sapiens
74 MALL  
Affinity Capture-MS Homo sapiens
75 USP33  
Affinity Capture-MS Homo sapiens
76 IGFL3  
Affinity Capture-MS Homo sapiens
77 C1orf189  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PRPF38B is involved
No pathways found





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