Gene description for CDC5L
Gene name cell division cycle 5-like
Gene symbol CDC5L
Other names/aliases CDC5
CDC5-LIKE
CEF1
PCDC5RP
dJ319D22.1
Species Homo sapiens
 Database cross references - CDC5L
ExoCarta ExoCarta_988
Entrez Gene 988
HGNC 1743
MIM 602868
UniProt Q99459  
 CDC5L identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Thymus 23844026    
 Gene ontology annotations for CDC5L
Molecular Function
    DNA binding GO:0003677 NAS
    poly(A) RNA binding GO:0044822 IDA
    chromatin binding GO:0003682 IEA
    protein binding GO:0005515 IPI
    WD40-repeat domain binding GO:0071987 IDA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 TAS
    signal transduction involved in DNA damage checkpoint GO:0072422 IMP
    regulation of transcription, DNA-templated GO:0006355 IEA
    gene expression GO:0010467 TAS
    DNA repair GO:0006281 IEA
    RNA splicing GO:0008380 TAS
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    DNA replication factor A complex GO:0005662 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    Prp19 complex GO:0000974 IDA
    catalytic step 2 spliceosome GO:0071013 IDA
    nucleolus GO:0005730 IDA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 IDA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified CDC5L in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDC5L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP2CA 5515
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 MCM5 4174
Affinity Capture-MS Homo sapiens
3 MCM10 55388
Affinity Capture-MS Homo sapiens
4 PLRG1 5356
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Protein-peptide Homo sapiens
5 MCM3 4172
Affinity Capture-MS Homo sapiens
6 ORC1L  
Affinity Capture-MS Homo sapiens
7 TTF2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 PPP1R8 5511
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 CDKN1A  
Affinity Capture-MS Homo sapiens
10 ORC2L  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CDC5L is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here