Gene description for UBB
Gene name ubiquitin B
Gene symbol UBB
Other names/aliases -
Species Homo sapiens
 Database cross references - UBB
ExoCarta ExoCarta_7314
Entrez Gene 7314
HGNC 12463
MIM 191339
UniProt P0CG47  
 UBB identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Breast milk 17641064    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
Tracheobronchial cells 19190083    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for UBB
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    I-kappaB kinase/NF-kappaB signaling GO:0007249 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    translesion synthesis GO:0019985 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I GO:0042590 TAS
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    seminiferous tubule development GO:0072520 IEA
    regulation of necrotic cell death GO:0010939 TAS
    fat pad development GO:0060613 IEA
    endosomal transport GO:0016197 TAS
    DNA repair GO:0006281 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    female meiosis I GO:0007144 IEA
    viral process GO:0016032 TAS
    error-free translesion synthesis GO:0070987 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 TAS
    viral life cycle GO:0019058 TAS
    Notch signaling pathway GO:0007219 TAS
    nucleotide-binding oligomerization domain containing signaling pathway GO:0070423 TAS
    positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator GO:1902255 IDA
    gene expression GO:0010467 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    male meiosis I GO:0007141 IEA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    negative regulation of type I interferon production GO:0032480 TAS
    ion transmembrane transport GO:0034220 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    hypothalamus gonadotrophin-releasing hormone neuron development GO:0021888 IEA
    small molecule metabolic process GO:0044281 TAS
    transcription, DNA-templated GO:0006351 TAS
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    ubiquitin homeostasis GO:0010992 IEA
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    cellular response to hypoxia GO:0071456 TAS
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
    programmed cell death GO:0012501 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    regulation of neuron death GO:1901214 IDA
    mitochondrion transport along microtubule GO:0047497 IDA
    transmembrane transport GO:0055085 TAS
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 TAS
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    programmed necrotic cell death GO:0097300 TAS
    neuron projection morphogenesis GO:0048812 IMP
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    regulation of mitochondrial membrane potential GO:0051881 IDA
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    negative regulation of apoptotic process GO:0043066 TAS
    JNK cascade GO:0007254 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    virion assembly GO:0019068 TAS
    glucose metabolic process GO:0006006 TAS
    glycogen biosynthetic process GO:0005978 TAS
    energy homeostasis GO:0097009 IEA
    positive regulation of apoptotic process GO:0043065 TAS
    regulation of proteasomal protein catabolic process GO:0061136 IDA
    regulation of apoptotic process GO:0042981 TAS
    circadian rhythm GO:0007623 TAS
    activation of MAPK activity GO:0000187 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 TAS
    apoptotic signaling pathway GO:0097190 TAS
    membrane organization GO:0061024 TAS
    carbohydrate metabolic process GO:0005975 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent GO:0002479 TAS
    regulation of type I interferon production GO:0032479 TAS
    innate immune response GO:0045087 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    apoptotic process GO:0006915 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    necroptotic process GO:0070266 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    protein polyubiquitination GO:0000209 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    error-prone translesion synthesis GO:0042276 TAS
    viral protein processing GO:0019082 TAS
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 TAS
    Notch receptor processing GO:0007220 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    female gonad development GO:0008585 IEA
    intracellular transport of virus GO:0075733 TAS
    DNA damage response, detection of DNA damage GO:0042769 TAS
    mitotic cell cycle GO:0000278 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    neuronal cell body GO:0043025 IDA
    nucleoplasm GO:0005654 TAS
    endosome membrane GO:0010008 TAS
    neuron projection GO:0043005 IDA
    endocytic vesicle membrane GO:0030666 TAS
 Experiment description of studies that identified UBB in exosomes
1
Experiment ID 76
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90
EV Cytosolic markers
✔
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 46
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|MHCII|CD81|CD86
EV Membrane markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
3
Experiment ID 20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
4
Experiment ID 21
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 254
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 255
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 256
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 257
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
13
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
14
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
20
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
21
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
22
Experiment ID 34
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD63|MHCI|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
23
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYBL2 4605
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
2 DAZAP2  
Two-hybrid Homo sapiens
3 IKBKG 8517
Affinity Capture-MS Homo sapiens
4 SYK 6850
Affinity Capture-MS Homo sapiens
5 TRAF6 7189
Affinity Capture-MS Homo sapiens
6 PARK2  
Affinity Capture-MS Homo sapiens
7 RNF11 26994
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which UBB is involved
PathwayEvidenceSource
Activated NOTCH1 Transmits Signal to the Nucleus IEA Reactome
Activated NOTCH1 Transmits Signal to the Nucleus TAS Reactome
activated TAK1 mediates p38 MAPK activation TAS Reactome
Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon TAS Reactome
Activation of NF-kappaB in B cells TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
APC-Cdc20 mediated degradation of Nek2A TAS Reactome
APC/C:Cdc20 mediated degradation of Cyclin B TAS Reactome
APC/C:Cdc20 mediated degradation of Securin TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
Assembly Of The HIV Virion TAS Reactome
Association of licensing factors with the pre-replicative complex TAS Reactome
Asymmetric localization of PCP proteins TAS Reactome
AUF1 (hnRNP D0) destabilizes mRNA TAS Reactome
Autodegradation of Cdh1 by Cdh1:APC/C TAS Reactome
Autodegradation of the E3 ubiquitin ligase COP1 TAS Reactome
Budding and maturation of HIV virion TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
CDT1 association with the CDC6:ORC:origin complex TAS Reactome
Circadian Clock TAS Reactome
CLEC7A (Dectin-1) signaling TAS Reactome
Constitutive Signaling by Ligand-Responsive EGFR Cancer Variants TAS Reactome
Constitutive Signaling by NOTCH1 HD Domain Mutants TAS Reactome
Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants TAS Reactome
Constitutive Signaling by NOTCH1 PEST Domain Mutants TAS Reactome
Cyclin D associated events in G1 TAS Reactome
deactivation of the beta-catenin transactivating complex TAS Reactome
Dectin-1 mediated noncanonical NF-kB signaling TAS Reactome
degradation of AXIN TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
degradation of DVL TAS Reactome
Degradation of GLI1 by the proteasome TAS Reactome
Degradation of GLI2 by the proteasome TAS Reactome
Downregulation of ERBB2:ERBB3 signaling TAS Reactome
Downregulation of ERBB4 signaling TAS Reactome
Downregulation of SMAD2/3:SMAD4 transcriptional activity TAS Reactome
Downregulation of TGF-beta receptor signaling TAS Reactome
Downstream TCR signaling TAS Reactome
EGFR downregulation TAS Reactome
Endosomal Sorting Complex Required For Transport (ESCRT) TAS Reactome
ER-Phagosome pathway TAS Reactome
Fanconi Anemia pathway TAS Reactome
FCERI mediated NF-kB activation TAS Reactome
GLI3 is processed to GLI3R by the proteasome TAS Reactome
Glycogen synthesis TAS Reactome
Hedgehog 'on' state TAS Reactome
Hedgehog ligand biogenesis TAS Reactome
Hh mutants that don't undergo autocatalytic processing are degraded by ERAD TAS Reactome
IKK complex recruitment mediated by RIP1 TAS Reactome
Interleukin-1 signaling TAS Reactome
IRAK1 recruits IKK complex TAS Reactome
IRAK1 recruits IKK complex TAS Reactome
IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation TAS Reactome
IRAK2 mediated activation of TAK1 complex TAS Reactome
IRAK2 mediated activation of TAK1 complex TAS Reactome
IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation TAS Reactome
ISG15 antiviral mechanism TAS Reactome
JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 TAS Reactome
Membrane binding and targetting of GAG proteins TAS Reactome
Myoclonic epilepsy of Lafora TAS Reactome
Negative regulation of FGFR1 signaling TAS Reactome
Negative regulation of FGFR2 signaling TAS Reactome
Negative regulation of FGFR3 signaling TAS Reactome
Negative regulation of FGFR4 signaling TAS Reactome
Negative regulators of RIG-I/MDA5 signaling TAS Reactome
NF-kB is activated and signals survival TAS Reactome
NOD1/2 Signaling Pathway TAS Reactome
NOTCH1 Intracellular Domain Regulates Transcription TAS Reactome
NOTCH2 Activation and Transmission of Signal to the Nucleus TAS Reactome
NRIF signals cell death from the nucleus TAS Reactome
Oncogene Induced Senescence TAS Reactome
Orc1 removal from chromatin TAS Reactome
Orc1 removal from chromatin TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha TAS Reactome
p75NTR recruits signalling complexes TAS Reactome
Recognition of DNA damage by PCNA-containing replication complex TAS Reactome
Regulation of activated PAK-2p34 by proteasome mediated degradation TAS Reactome
regulation of FZD by ubiquitination TAS Reactome
Regulation of innate immune responses to cytosolic DNA TAS Reactome
Regulation of necroptotic cell death TAS Reactome
Regulation of PLK1 Activity at G2/M Transition TAS Reactome
Regulation of signaling by CBL TAS Reactome
Regulation of the Fanconi anemia pathway TAS Reactome
RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
SCF-beta-TrCP mediated degradation of Emi1 TAS Reactome
Senescence-Associated Secretory Phenotype (SASP) TAS Reactome
Separation of Sister Chromatids TAS Reactome
Signaling by ERBB2 TAS Reactome
SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Stimuli-sensing channels TAS Reactome
TAK1 activates NFkB by phosphorylation and activation of IKKs complex TAS Reactome
TCF dependent signaling in response to WNT TAS Reactome
Termination of translesion DNA synthesis TAS Reactome
TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) TAS Reactome
TRAF6 mediated induction of TAK1 complex TAS Reactome
TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling TAS Reactome
Translesion Synthesis by POLH TAS Reactome
Translesion synthesis by POLI TAS Reactome
Translesion synthesis by POLK TAS Reactome
Translesion synthesis by REV1 TAS Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A TAS Reactome
Ubiquitin-dependent degradation of Cyclin D1 TAS Reactome
Vif-mediated degradation of APOBEC3G TAS Reactome
Vpu mediated degradation of CD4 TAS Reactome





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