Gene description for GIGYF2
Gene name GRB10 interacting GYF protein 2
Gene symbol GIGYF2
Other names/aliases GYF2
PARK11
PERQ2
PERQ3
TNRC15
Species Homo sapiens
 Database cross references - GIGYF2
ExoCarta ExoCarta_26058
Vesiclepedia VP_26058
Entrez Gene 26058
HGNC 11960
MIM 612003
UniProt Q6Y7W6  
 GIGYF2 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GIGYF2
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    cadherin binding GO:0045296 HDA
    molecular adaptor activity GO:0060090 IDA
    proline-rich region binding GO:0070064 IDA
Biological Process
    feeding behavior GO:0007631 IEA
    adult locomotory behavior GO:0008344 IEA
    post-embryonic development GO:0009791 IEA
    post-transcriptional gene silencing GO:0016441 IDA
    negative regulation of translation GO:0017148 IMP
    spinal cord motor neuron differentiation GO:0021522 IEA
    mitotic G1 DNA damage checkpoint signaling GO:0031571 IEA
    multicellular organism growth GO:0035264 IEA
    negative regulation of translational initiation GO:0045947 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IBA
    insulin-like growth factor receptor signaling pathway GO:0048009 IMP
    homeostasis of number of cells within a tissue GO:0048873 IEA
    musculoskeletal movement GO:0050881 IEA
    neuromuscular process controlling balance GO:0050885 IEA
    negative regulation of type I interferon-mediated signaling pathway GO:0060339 IDA
    mRNA destabilization GO:0061157 IDA
    rescue of stalled ribosome GO:0072344 IDA
Subcellular Localization
    endosome GO:0005768 IDA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytoplasmic stress granule GO:0010494 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    vesicle GO:0031982 IBA
    protein-containing complex GO:0032991 IDA
    perikaryon GO:0043204 IBA
    perikaryon GO:0043204 IDA
    proximal dendrite GO:1990635 IBA
    proximal dendrite GO:1990635 IDA
 Experiment description of studies that identified GIGYF2 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GIGYF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 EXOSC7 23016
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 LGR4 55366
Affinity Capture-MS Homo sapiens
4 KSR1  
Affinity Capture-MS Homo sapiens
5 RALGPS2 55103
Affinity Capture-MS Homo sapiens
6 ANLN 54443
Affinity Capture-MS Homo sapiens
7 MCM9 254394
Cross-Linking-MS (XL-MS) Homo sapiens
8 TARDBP 23435
Affinity Capture-MS Homo sapiens
9 YWHAE 7531
Affinity Capture-MS Homo sapiens
10 ANAPC15  
Affinity Capture-MS Homo sapiens
11 RPS15 6209
Cross-Linking-MS (XL-MS) Homo sapiens
12 APC  
Two-hybrid Homo sapiens
13 NAV1 89796
Affinity Capture-MS Homo sapiens
14 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
15 KIF20A 10112
Affinity Capture-MS Homo sapiens
16 SRGAP2 23380
Affinity Capture-MS Homo sapiens
17 GRB10 2887
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 CAMSAP2  
Affinity Capture-MS Homo sapiens
19 CBY1  
Affinity Capture-MS Homo sapiens
20 ATN1  
Affinity Capture-MS Homo sapiens
21 HDAC4  
Affinity Capture-MS Homo sapiens
22 EIF4E2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
23 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
24 KIF13B 23303
Affinity Capture-MS Homo sapiens
25 TOP2A 7153
Affinity Capture-MS Homo sapiens
26 RPS20 6224
Proximity Label-MS Homo sapiens
27 EXOSC2 23404
Affinity Capture-MS Homo sapiens
28 C1D  
Affinity Capture-MS Homo sapiens
29 ZBTB21  
Affinity Capture-MS Homo sapiens
30 PCK1 5105
Affinity Capture-MS Homo sapiens
31 D2HGDH 728294
Affinity Capture-MS Homo sapiens
32 RASAL2 9462
Affinity Capture-MS Homo sapiens
33 ZNF638 27332
Affinity Capture-MS Homo sapiens
34 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 FBXW7  
Affinity Capture-MS Homo sapiens
36 AKR7L 246181
Affinity Capture-MS Homo sapiens
37 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SNRPC 6631
Affinity Capture-MS Homo sapiens
39 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SMC2 10592
Co-fractionation Homo sapiens
41 TIAM1  
Affinity Capture-MS Homo sapiens
42 PHLDB2 90102
Affinity Capture-MS Homo sapiens
43 MAGED2 10916
Affinity Capture-MS Homo sapiens
44 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
45 EXOSC4 54512
Affinity Capture-MS Homo sapiens
46 TESK2  
Affinity Capture-MS Homo sapiens
47 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CEP57  
Affinity Capture-MS Homo sapiens
49 LRFN1  
Affinity Capture-MS Homo sapiens
50 ZNF598 90850
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 EXOSC6 118460
Affinity Capture-MS Homo sapiens
52 Eif3a 13669
Affinity Capture-MS Mus musculus
53 MPHOSPH6 10200
Affinity Capture-MS Homo sapiens
54 NPAS1  
Affinity Capture-MS Homo sapiens
55 RPS11 6205
Affinity Capture-MS Homo sapiens
56 MTA2 9219
Co-fractionation Homo sapiens
57 KIAA1804  
Affinity Capture-MS Homo sapiens
58 EXOSC9 5393
Affinity Capture-MS Homo sapiens
59 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 CPEB1  
Proximity Label-MS Homo sapiens
61 RFX5 5993
Affinity Capture-MS Homo sapiens
62 PRPF6 24148
Affinity Capture-MS Homo sapiens
63 DIS3L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FAM96A  
Affinity Capture-MS Homo sapiens
65 SULT1C4  
Affinity Capture-MS Homo sapiens
66 MIF 4282
Affinity Capture-MS Homo sapiens
67 MIB1 57534
Proximity Label-MS Homo sapiens
68 PRPF8 10594
Affinity Capture-MS Homo sapiens
69 EMD 2010
Affinity Capture-MS Homo sapiens
70 USP11 8237
Affinity Capture-MS Homo sapiens
71 CDKN1A  
Affinity Capture-MS Homo sapiens
72 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
73 MEX3C 51320
Affinity Capture-MS Homo sapiens
74 RBM7  
Affinity Capture-MS Homo sapiens
75 MAPRE1 22919
Affinity Capture-MS Homo sapiens
76 MICU2 221154
Affinity Capture-MS Homo sapiens
77 TRIM37  
Proximity Label-MS Homo sapiens
78 CGN  
Affinity Capture-MS Homo sapiens
79 C12orf49  
Positive Genetic Homo sapiens
80 DDX20 11218
Affinity Capture-MS Homo sapiens
81 PABPC1 26986
Affinity Capture-MS Homo sapiens
82 CDH1 999
Proximity Label-MS Homo sapiens
83 RPA2 6118
Proximity Label-MS Homo sapiens
84 MYH9 4627
Co-fractionation Homo sapiens
85 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
86 NTRK1 4914
Affinity Capture-MS Homo sapiens
87 U2AF2 11338
Affinity Capture-MS Homo sapiens
88 MYO1B 4430
Cross-Linking-MS (XL-MS) Homo sapiens
89 EXOSC5 56915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 LPIN3 64900
Affinity Capture-MS Homo sapiens
91 RPL36 25873
Cross-Linking-MS (XL-MS) Homo sapiens
92 HUWE1 10075
Affinity Capture-MS Homo sapiens
93 TGM1 7051
Affinity Capture-MS Homo sapiens
94 PRKAR2A 5576
Co-fractionation Homo sapiens
95 CEP76  
Affinity Capture-MS Homo sapiens
96 EPB41L5 57669
Affinity Capture-MS Homo sapiens
97 TANC2  
Affinity Capture-MS Homo sapiens
98 KCTD3  
Affinity Capture-MS Homo sapiens
99 DDOST 1650
Affinity Capture-MS Homo sapiens
100 MCM2 4171
Affinity Capture-MS Homo sapiens
101 RPA3 6119
Proximity Label-MS Homo sapiens
102 ZCCHC8 55596
Affinity Capture-MS Homo sapiens
103 PHLPP1  
Proximity Label-MS Homo sapiens
104 LIMA1 51474
Affinity Capture-MS Homo sapiens
105 MRM1  
Affinity Capture-MS Homo sapiens
106 SF3B2 10992
Affinity Capture-MS Homo sapiens
107 DCLK1 9201
Affinity Capture-MS Homo sapiens
108 HBS1L 10767
Affinity Capture-MS Homo sapiens
109 RPL7A 6130
Cross-Linking-MS (XL-MS) Homo sapiens
110 XPO1 7514
Affinity Capture-MS Homo sapiens
111 PIP 5304
Affinity Capture-MS Homo sapiens
112 RC3H2  
Affinity Capture-MS Homo sapiens
113 ECT2 1894
Affinity Capture-MS Homo sapiens
114 EXOSC10 5394
Affinity Capture-MS Homo sapiens
115 SIRT7  
Affinity Capture-MS Homo sapiens
116 NCAPH2  
Affinity Capture-MS Homo sapiens
117 OSBPL6  
Affinity Capture-MS Homo sapiens
118 INPP5E 56623
Affinity Capture-MS Homo sapiens
119 RQCD1 9125
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
120 YWHAZ 7534
Affinity Capture-MS Homo sapiens
121 EGFR 1956
Affinity Capture-MS Homo sapiens
122 MAB21L2  
Affinity Capture-MS Homo sapiens
123 NADK 65220
Affinity Capture-MS Homo sapiens
124 PNMA2  
Affinity Capture-MS Homo sapiens
125 DUSP16  
Affinity Capture-MS Homo sapiens
126 TNRC6B  
Affinity Capture-MS Homo sapiens
127 AURKB 9212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 CDC25C  
Affinity Capture-MS Homo sapiens
129 CEP250 11190
Affinity Capture-MS Homo sapiens
130 PCBP1 5093
Proximity Label-MS Homo sapiens
131 PTPN13 5783
Affinity Capture-MS Homo sapiens
132 NIN 51199
Proximity Label-MS Homo sapiens
133 GIGYF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 EXOSC3 51010
Affinity Capture-MS Homo sapiens
135 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
136 BICD1 636
Proximity Label-MS Homo sapiens
137 BMI1  
Affinity Capture-MS Homo sapiens
138 Rpl35 66489
Affinity Capture-MS Mus musculus
139 NEUROG3  
Affinity Capture-MS Homo sapiens
140 UPF2 26019
Cross-Linking-MS (XL-MS) Homo sapiens
141 CCDC8  
Affinity Capture-MS Homo sapiens
142 GEMIN4 50628
Affinity Capture-MS Homo sapiens
143 OST4  
Affinity Capture-MS Homo sapiens
144 DENND4C 55667
Affinity Capture-MS Homo sapiens
145 YBX1 4904
Affinity Capture-MS Homo sapiens
146 RNF43  
Proximity Label-MS Homo sapiens
147 SH3RF3  
Affinity Capture-MS Homo sapiens
148 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
149 FASN 2194
Negative Genetic Homo sapiens
150 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
151 SMN1 6606
Affinity Capture-MS Homo sapiens
152 WDR77 79084
Affinity Capture-MS Homo sapiens
153 TP53 7157
Affinity Capture-MS Homo sapiens
154 RTKN 6242
Affinity Capture-MS Homo sapiens
155 U2AF1L4 199746
Affinity Capture-MS Homo sapiens
156 Parg  
Affinity Capture-MS Mus musculus
157 DENND1A 57706
Affinity Capture-MS Homo sapiens
158 PPM1H  
Affinity Capture-MS Homo sapiens
159 FXR1 8087
Affinity Capture-MS Homo sapiens
160 APEX1 328
Affinity Capture-RNA Homo sapiens
161 THOP1 7064
Affinity Capture-MS Homo sapiens
162 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
163 NINL  
Proximity Label-MS Homo sapiens
164 MAGEL2  
Proximity Label-MS Homo sapiens
165 G3BP1 10146
Affinity Capture-MS Homo sapiens
166 MAGI1  
Affinity Capture-MS Homo sapiens
167 ELAVL1 1994
Affinity Capture-MS Homo sapiens
168 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
169 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
170 AGO2 27161
Affinity Capture-MS Homo sapiens
171 DDX58 23586
Affinity Capture-RNA Homo sapiens
172 SQSTM1 8878
Proximity Label-MS Homo sapiens
173 C20orf197  
Affinity Capture-MS Homo sapiens
174 NF1 4763
Affinity Capture-MS Homo sapiens
175 C10orf88  
Affinity Capture-MS Homo sapiens
176 CAD 790
Co-fractionation Homo sapiens
177 AIRE  
Affinity Capture-MS Homo sapiens
178 TRIM31  
Affinity Capture-MS Homo sapiens
179 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
180 SERBP1 26135
Proximity Label-MS Homo sapiens
181 KIF1C 10749
Affinity Capture-MS Homo sapiens
182 MAST3  
Affinity Capture-MS Homo sapiens
183 PRMT1 3276
Affinity Capture-MS Homo sapiens
184 EXOSC1 51013
Affinity Capture-MS Homo sapiens
185 WDHD1  
Co-fractionation Homo sapiens
186 EXOSC8  
Affinity Capture-MS Homo sapiens
187 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GIGYF2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here