Gene description for HSPD1
Gene name heat shock 60kDa protein 1 (chaperonin)
Gene symbol HSPD1
Other names/aliases CPN60
GROEL
HLD4
HSP-60
HSP60
HSP65
HuCHA60
SPG13
Species Homo sapiens
 Database cross references - HSPD1
ExoCarta ExoCarta_3329
Vesiclepedia VP_3329
Entrez Gene 3329
HGNC 5261
MIM 118190
UniProt P10809  
 HSPD1 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 19415654    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct fluid 29339034    
Pancreatic duct fluid 29339034    
Pluripotent stem cells 34108659    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25844599    
T lymphocytes 34108659    
Trophoblast cells 21276792    
Urine 15326289    
Urine 19056867    
 Gene ontology annotations for HSPD1
Molecular Function
    lipopolysaccharide binding GO:0001530 IDA
    p53 binding GO:0002039 IPI
    DNA replication origin binding GO:0003688 ISS
    single-stranded DNA binding GO:0003697 ISS
    RNA binding GO:0003723 HDA
    double-stranded RNA binding GO:0003725 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    high-density lipoprotein particle binding GO:0008035 IDA
    isomerase activity GO:0016853 IEA
    ATP hydrolysis activity GO:0016887 ISS
    enzyme binding GO:0019899 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    apolipoprotein binding GO:0034185 IPI
    apolipoprotein A-I binding GO:0034186 IPI
    unfolded protein binding GO:0051082 IC
    unfolded protein binding GO:0051082 ISS
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 IPI
    cysteine-type endopeptidase activator activity GO:0140608 IDA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 IDA
    positive regulation of T cell mediated immune response to tumor cell GO:0002842 IDA
    protein folding GO:0006457 IBA
    'de novo' protein folding GO:0006458 ISS
    response to unfolded protein GO:0006986 IDA
    apoptotic mitochondrial changes GO:0008637 IBA
    response to cold GO:0009409 ISS
    positive regulation of interferon-alpha production GO:0032727 IBA
    positive regulation of interferon-alpha production GO:0032727 IDA
    positive regulation of type II interferon production GO:0032729 IBA
    positive regulation of type II interferon production GO:0032729 IDA
    positive regulation of type II interferon production GO:0032729 IDA
    positive regulation of type II interferon production GO:0032729 ISS
    positive regulation of interleukin-10 production GO:0032733 IDA
    positive regulation of interleukin-12 production GO:0032735 IDA
    positive regulation of interleukin-6 production GO:0032755 IBA
    positive regulation of interleukin-6 production GO:0032755 IDA
    mitochondrial unfolded protein response GO:0034514 IBA
    protein refolding GO:0042026 IDA
    B cell proliferation GO:0042100 IDA
    T cell activation GO:0042110 IBA
    T cell activation GO:0042110 IDA
    B cell activation GO:0042113 IDA
    positive regulation of macrophage activation GO:0043032 IDA
    negative regulation of apoptotic process GO:0043066 IMP
    adhesion of symbiont to host GO:0044406 IDA
    protein import into mitochondrial intermembrane space GO:0045041 IBA
    isotype switching to IgG isotypes GO:0048291 IDA
    protein stabilization GO:0050821 IMP
    protein stabilization GO:0050821 ISS
    positive regulation of T cell activation GO:0050870 IBA
    positive regulation of T cell activation GO:0050870 IDA
    positive regulation of T cell activation GO:0050870 ISS
    chaperone-mediated protein complex assembly GO:0051131 ISS
    protein maturation GO:0051604 ISS
    biological process involved in interaction with symbiont GO:0051702 IMP
    cellular response to interleukin-7 GO:0098761 IEA
    negative regulation of execution phase of apoptosis GO:1900118 IMP
    positive regulation of execution phase of apoptosis GO:1900119 IMP
Subcellular Localization
    extracellular space GO:0005615 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 TAS
    early endosome GO:0005769 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 IDA
    cell surface GO:0009986 IDA
    membrane GO:0016020 HDA
    coated vesicle GO:0030135 IDA
    secretory granule GO:0030141 ISS
    protein-containing complex GO:0032991 IDA
    lipopolysaccharide receptor complex GO:0046696 IDA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
    sperm midpiece GO:0097225 IMP
    sperm plasma membrane GO:0097524 IEA
    migrasome GO:0140494 IEA
 Experiment description of studies that identified HSPD1 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 65
MISEV standards
✘ Biophysical techniques
✔
HSP60|HSP27|GAPDH|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors "Staubach S, Razawi H, Hanisch FG."
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
9
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
11
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
15
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
16
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
18
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
19
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
20
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
21
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
22
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
23
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
24
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
31
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
32
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
34
Experiment ID 266
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
35
Experiment ID 268
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
36
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
38
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
39
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
40
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
41
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
42
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
43
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
44
Experiment ID 219
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name C666-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
45
Experiment ID 220
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP69
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
46
Experiment ID 221
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP460
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
47
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
48
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
49
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
50
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
51
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
52
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
53
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
54
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
55
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
56
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
57
Experiment ID 622
MISEV standards
✔
EM
Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with Pancreatic ductal adenocarcinoma
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
58
Experiment ID 623
MISEV standards
✘ Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with IPMN with microinvasive cancer (IPMNca)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
59
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
60
Experiment ID 143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
61
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
62
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
63
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
64
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
65
Experiment ID 121
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|HSP60|HSP70|CD81
Enriched markers
✔
HSPA5
Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21276792    
Organism Homo sapiens
Experiment description Morphologic and proteomic characterization of exosomes released by cultured extravillous trophoblast cells.
Authors "Atay S, Gercel-Taylor C, Kesimer M, Taylor DD."
Journal name ECR
Publication year 2011
Sample Trophoblast cells
Sample name Sw71
Isolation/purification methods Differential centrifugation
Ultrafiltration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
66
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
67
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HSPD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIRT4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-Western Homo sapiens
3 ITGB1 3688
Co-fractionation Homo sapiens
4 ISG15 9636
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 OXCT1 5019
Co-fractionation Homo sapiens
6 TRMT1L 81627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HDAC2 3066
Affinity Capture-MS Homo sapiens
8 UBL4A 8266
Affinity Capture-MS Homo sapiens
9 RIN3  
Affinity Capture-MS Homo sapiens
10 SKI 6497
Affinity Capture-MS Homo sapiens
11 PPP1R3D  
Co-fractionation Homo sapiens
12 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
13 CAP1 10487
Co-fractionation Homo sapiens
14 DDX17 10521
Co-fractionation Homo sapiens
15 EBNA-LP  
Affinity Capture-Western
Affinity Capture-MS
16 BRCA1 672
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
17 MTG2  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KIF20A 10112
Affinity Capture-MS Homo sapiens
19 OXNAD1 92106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 UBC 7316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
22 HDAC4  
Affinity Capture-MS Homo sapiens
23 SOX2  
Affinity Capture-MS Homo sapiens
24 AKAP8L  
Affinity Capture-MS Homo sapiens
25 CCDC90B  
Proximity Label-MS Homo sapiens
26 SEPT9 10801
Co-fractionation Homo sapiens
27 FOXRED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 WDR76  
Affinity Capture-MS Homo sapiens
29 PFKL 5211
Co-fractionation Homo sapiens
30 TRMU  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 KIF23 9493
Affinity Capture-MS Homo sapiens
32 BCAT2 587
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 GNS 2799
Affinity Capture-MS Homo sapiens
34 AMFR 267
Affinity Capture-MS Homo sapiens
35 PTMS 5763
Co-fractionation Homo sapiens
36 PUSL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 ABHD16B  
Affinity Capture-MS Homo sapiens
38 FBL 2091
Cross-Linking-MS (XL-MS) Homo sapiens
39 ASB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
40 KLHL33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 KRT31 3881
Two-hybrid Homo sapiens
42 FAM207A  
Cross-Linking-MS (XL-MS) Homo sapiens
43 RAB1A 5861
Co-fractionation Homo sapiens
44 SNRPD3 6634
Co-fractionation Homo sapiens
45 HMG20B  
Co-fractionation Homo sapiens
46 MECP2 4204
Affinity Capture-MS Homo sapiens
47 EEF1A1 1915
Co-fractionation Homo sapiens
48 KIF14 9928
Affinity Capture-MS Homo sapiens
49 UNK  
Affinity Capture-RNA Homo sapiens
50 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 AURKA 6790
Affinity Capture-MS Homo sapiens
52 MTCH2 23788
Affinity Capture-MS Homo sapiens
53 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
54 USP11 8237
Affinity Capture-MS Homo sapiens
55 TMEM177  
Affinity Capture-MS Homo sapiens
56 CLEC7A  
Two-hybrid Homo sapiens
57 CLU 1191
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
58 C17orf49 124944
Cross-Linking-MS (XL-MS) Homo sapiens
59 C17orf85  
Cross-Linking-MS (XL-MS) Homo sapiens
60 TUFM 7284
Co-fractionation Homo sapiens
61 HIST1H3E 8353
Co-purification Homo sapiens
62 ESRRB  
Affinity Capture-MS Homo sapiens
63 PABPC1 26986
Co-fractionation Homo sapiens
64 CDH1 999
Affinity Capture-MS Homo sapiens
65 YAP1 10413
Affinity Capture-MS Homo sapiens
66 MYH9 4627
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
67 TRIM21 6737
Affinity Capture-MS Homo sapiens
68 OGT 8473
Reconstituted Complex Homo sapiens
69 SYNCRIP 10492
Co-fractionation Homo sapiens
70 NOS2  
Affinity Capture-MS Homo sapiens
71 BICD2 23299
Proximity Label-MS Homo sapiens
72 P4HB 5034
Co-fractionation Homo sapiens
73 SNRPD1 6632
Co-fractionation Homo sapiens
74 B4GALT3 8703
Affinity Capture-MS Homo sapiens
75 CUL2 8453
Affinity Capture-MS Homo sapiens
76 ACTC1 70
Affinity Capture-MS Homo sapiens
77 DDX39B 7919
Co-fractionation Homo sapiens
78 TRIM63  
Biochemical Activity Homo sapiens
79 U2SURP 23350
Co-fractionation Homo sapiens
80 GATA3  
Affinity Capture-MS Homo sapiens
81 SCARNA22  
Affinity Capture-RNA Homo sapiens
82 ANLN 54443
Affinity Capture-MS Homo sapiens
83 ARSA 410
Affinity Capture-MS Homo sapiens
84 TMPO 7112
Affinity Capture-MS Homo sapiens
85 RARS2  
Affinity Capture-MS Homo sapiens
86 PNPLA4  
Affinity Capture-MS Homo sapiens
87 GMPPB 29925
Co-fractionation Homo sapiens
88 MTIF2 4528
Proximity Label-MS Homo sapiens
89 MCCC2 64087
Co-fractionation Homo sapiens
90 C9orf78 51759
Affinity Capture-MS Homo sapiens
91 SDHA 6389
Co-fractionation Homo sapiens
92 RYR3 6263
Cross-Linking-MS (XL-MS) Homo sapiens
93 Wdr5  
Affinity Capture-MS Mus musculus
94 IL25  
Affinity Capture-MS Homo sapiens
95 MYH14 79784
Cross-Linking-MS (XL-MS) Homo sapiens
96 NEU4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 MRPL16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 CUL3 8452
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 COQ4  
Affinity Capture-MS Homo sapiens
100 FAHD1 81889
Affinity Capture-MS Homo sapiens
101 EIF4E 1977
Co-fractionation Homo sapiens
102 EPRS 2058
Co-fractionation Homo sapiens
103 FN1 2335
Affinity Capture-MS Homo sapiens
104 C4orf29 80167
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 MTG1  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 RPA3 6119
Proximity Label-MS Homo sapiens
107 CDC20 991
Affinity Capture-MS Homo sapiens
108 GSTK1 373156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 PHF5A 84844
Co-fractionation Homo sapiens
110 HSPA1A 3303
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
111 HSPA4 3308
Co-fractionation Homo sapiens
112 SLC25A18  
Affinity Capture-MS Homo sapiens
113 RFWD3  
Affinity Capture-MS Homo sapiens
114 MARS2 92935
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 GBAS 2631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 STUB1 10273
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
117 DDX46 9879
Co-fractionation Homo sapiens
118 FTL 2512
Co-fractionation Homo sapiens
119 HSPE1 3336
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
120 FBXL6  
Affinity Capture-MS Homo sapiens
121 AUH 549
Proximity Label-MS Homo sapiens
122 PDE4B  
Protein-RNA Homo sapiens
123 GRB2 2885
Affinity Capture-MS Homo sapiens
124 COQ5  
Affinity Capture-MS Homo sapiens
125 STRN4 29888
Co-fractionation Homo sapiens
126 LRRC28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
128 PTPN2 5771
Co-fractionation Homo sapiens
129 SAMD3  
Two-hybrid Homo sapiens
130 NUDT19 390916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 PUF60 22827
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
132 SIRT1  
Affinity Capture-MS Homo sapiens
133 SSBP1 6742
Co-fractionation Homo sapiens
134 GORASP1 64689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 GLB1L3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 GAPDH 2597
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
137 HNRNPL 3191
Co-fractionation Homo sapiens
138 SUPT6H 6830
Reconstituted Complex Homo sapiens
139 EIF4B 1975
Co-fractionation Homo sapiens
140 FECH 2235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 REXO2 25996
Co-fractionation Homo sapiens
142 ACSF3 197322
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 DNAH10 196385
Cross-Linking-MS (XL-MS) Homo sapiens
144 SNW1 22938
Affinity Capture-MS Homo sapiens
145 COLGALT1 79709
Co-fractionation Homo sapiens
146 SNRPA1 6627
Co-fractionation Homo sapiens
147 HSPA5 3309
Co-fractionation Homo sapiens
148 TRIM43  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 TTC39B 158219
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
151 SLC25A39 51629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
152 PMPCA 23203
Proximity Label-MS Homo sapiens
153 SPRTN  
Affinity Capture-MS Homo sapiens
154 BCKDK 10295
Affinity Capture-MS Homo sapiens
155 GTPBP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 MAGEA3  
Affinity Capture-MS Homo sapiens
157 GART 2618
Co-fractionation Homo sapiens
158 FBXO7 25793
Affinity Capture-MS Homo sapiens
159 BTF3 689
Affinity Capture-MS Homo sapiens
160 ADRB2  
Affinity Capture-MS Homo sapiens
161 ALDH2 217
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
162 PACSIN2 11252
Co-fractionation Homo sapiens
163 DTNA  
Affinity Capture-MS Homo sapiens
164 PHOSPHO1  
Affinity Capture-MS Homo sapiens
165 HSP90AB1 3326
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
166 CAND1 55832
Affinity Capture-MS Homo sapiens
167 ASXL1  
Affinity Capture-MS Homo sapiens
168 CPT1A 1374
Affinity Capture-MS Homo sapiens
169 CCDC65  
Cross-Linking-MS (XL-MS) Homo sapiens
170 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
171 WDR86  
Affinity Capture-MS Homo sapiens
172 B3GNT2 10678
Affinity Capture-MS Homo sapiens
173 KRT40  
Two-hybrid Homo sapiens
174 TRMT2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
175 HARS2 23438
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
176 FBXO17 115290
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
177 SRRM2 23524
Affinity Capture-MS Homo sapiens
178 MAPK14 1432
Co-fractionation Homo sapiens
179 OBSL1 23363
Affinity Capture-MS Homo sapiens
180 BAX 581
Affinity Capture-Western Homo sapiens
181 NSUN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
182 STIP1 10963
Co-fractionation Homo sapiens
183 RGS20 8601
Two-hybrid Homo sapiens
184 DNAJC7 7266
Co-fractionation Homo sapiens
185 VCAM1 7412
Affinity Capture-MS Homo sapiens
186 NAGK 55577
Co-fractionation Homo sapiens
187 TTLL5  
Affinity Capture-MS Homo sapiens
188 CUL1 8454
Affinity Capture-Western Homo sapiens
189 HSD17B4 3295
Co-fractionation Homo sapiens
190 PMPCB 9512
Proximity Label-MS Homo sapiens
191 FASTKD2  
Proximity Label-MS Homo sapiens
192 FTSJ2 29960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
193 C9orf163  
Affinity Capture-MS Homo sapiens
194 GDA 9615
Co-fractionation Homo sapiens
195 ITLN1 55600
Affinity Capture-MS Homo sapiens
196 TMA7 51372
Cross-Linking-MS (XL-MS) Homo sapiens
197 VRK3 51231
Affinity Capture-MS Homo sapiens
198 SETD9  
Affinity Capture-MS Homo sapiens
199 SLC25A10 1468
Affinity Capture-MS Homo sapiens
200 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
201 CDKN1A  
Co-fractionation Homo sapiens
202 METTL8  
Affinity Capture-MS Homo sapiens
203 OPTN 10133
Co-localization Homo sapiens
204 AMACR 23600
Affinity Capture-MS Homo sapiens
205 PSMD14 10213
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
206 SF3B1 23451
Co-fractionation Homo sapiens
207 VRK1 7443
Affinity Capture-MS Homo sapiens
208 TUBA1C 84790
Affinity Capture-MS Homo sapiens
209 UQCRC2 7385
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
210 CCT8 10694
Co-fractionation Homo sapiens
211 TEPP  
Affinity Capture-MS Homo sapiens
212 WNT5A 7474
Affinity Capture-MS Homo sapiens
213 THBS3 7059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
214 NCL 4691
Co-fractionation Homo sapiens
215 DCLRE1B  
Affinity Capture-MS Homo sapiens
216 GALNS 2588
Affinity Capture-MS Homo sapiens
217 CYLD  
Affinity Capture-MS Homo sapiens
218 SNRPB2 6629
Co-fractionation Homo sapiens
219 FBXL4 26235
Affinity Capture-MS Homo sapiens
220 KIF3B 9371
Cross-Linking-MS (XL-MS) Homo sapiens
221 PTPN11 5781
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
222 PSMA3 5684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
223 IBA57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
224 IKZF3  
Affinity Capture-MS Homo sapiens
225 PPA1 5464
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
226 KRTAP10-8  
Two-hybrid Homo sapiens
227 ATP5F1 515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
228 TUBB4B 10383
Co-fractionation Homo sapiens
229 NUP43 348995
Proximity Label-MS Homo sapiens
230 PACSIN3 29763
Co-fractionation Homo sapiens
231 FUS 2521
Affinity Capture-MS Homo sapiens
232 LAMB3 3914
Affinity Capture-MS Homo sapiens
233 IKZF1  
Affinity Capture-MS Homo sapiens
234 RIPK4  
Affinity Capture-MS Homo sapiens
235 LARS 51520
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
236 SPG20 23111
Affinity Capture-MS Homo sapiens
237 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
238 MAP2K3 5606
Affinity Capture-MS Homo sapiens
239 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
240 UBASH3A 53347
Reconstituted Complex Homo sapiens
241 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
242 ACAD9 28976
Proximity Label-MS Homo sapiens
243 CUL7 9820
Affinity Capture-MS Homo sapiens
244 DDRGK1 65992
Affinity Capture-MS Homo sapiens
245 XRCC3  
Affinity Capture-MS Homo sapiens
246 HMG20A  
Affinity Capture-MS Homo sapiens
247 SIRT3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
248 MCUR1 63933
Proximity Label-MS Homo sapiens
249 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
250 PHGDH 26227
Affinity Capture-MS Homo sapiens
251 GSR 2936
Co-fractionation Homo sapiens
252 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
253 CS 1431
Proximity Label-MS Homo sapiens
254 PSMB10 5699
Affinity Capture-MS Homo sapiens
255 SPOP  
Affinity Capture-MS Homo sapiens
256 ITGB5 3693
Cross-Linking-MS (XL-MS) Homo sapiens
257 CEP170P1  
Affinity Capture-MS Homo sapiens
258 PREP 5550
Co-fractionation Homo sapiens
259 TANGO2  
Co-fractionation Homo sapiens
260 FOLR1 2348
Affinity Capture-MS Homo sapiens
261 SF3B4 10262
Co-fractionation Homo sapiens
262 THOP1 7064
Co-fractionation Homo sapiens
263 PPP2R2C 5522
Affinity Capture-MS Homo sapiens
264 INSIG2  
Affinity Capture-MS Homo sapiens
265 NIPSNAP3B 55335
Affinity Capture-MS Homo sapiens
266 TFB1M  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
267 ESR1  
Affinity Capture-MS Homo sapiens
268 HEXIM1 10614
Co-fractionation Homo sapiens
269 FASTKD3  
Proximity Label-MS Homo sapiens
270 NCAPD2 9918
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
271 DHFR 1719
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
272 GFM1 85476
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
273 BCL2L1 598
Affinity Capture-MS Homo sapiens
274 CXorf56  
Co-fractionation Homo sapiens
275 SLC9A3R1 9368
Co-fractionation Homo sapiens
276 C1QBP 708
Affinity Capture-MS Homo sapiens
277 KRAS 3845
Affinity Capture-MS Homo sapiens
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
278 DCAF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
279 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
280 KRT34  
Two-hybrid Homo sapiens
281 PRMT1 3276
Affinity Capture-MS Homo sapiens
282 EEF1B2 1933
Co-fractionation Homo sapiens
283 CUL4A 8451
Affinity Capture-MS Homo sapiens
284 DARS2 55157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
285 GATA1  
Affinity Capture-MS Homo sapiens
286 BPHL 670
Affinity Capture-MS Homo sapiens
287 Hax1  
Affinity Capture-MS Mus musculus
288 SNRPE 6635
Co-fractionation Homo sapiens
289 ANKRD18A  
Cross-Linking-MS (XL-MS) Homo sapiens
290 DNAJC8 22826
Co-fractionation Homo sapiens
291 MARCKS 4082
Cross-Linking-MS (XL-MS) Homo sapiens
292 ACAD8 27034
Affinity Capture-MS Homo sapiens
293 PARD3 56288
Affinity Capture-MS Homo sapiens
294 CD4 920
Affinity Capture-MS Homo sapiens
295 GATA2  
Affinity Capture-MS Homo sapiens
296 EFTUD1 79631
Cross-Linking-MS (XL-MS) Homo sapiens
297 RPS6KB2  
Affinity Capture-MS Homo sapiens
298 TRIB2 28951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
299 SF3B5 83443
Co-fractionation Homo sapiens
300 Gnb1 14688
Affinity Capture-MS Mus musculus
301 CAPZB 832
Affinity Capture-MS Homo sapiens
302 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
303 PLOD3 8985
Co-fractionation Homo sapiens
304 MCM2 4171
Affinity Capture-MS Homo sapiens
305 CASP3 836
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
306 D2HGDH 728294
Affinity Capture-MS Homo sapiens
307 YARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
308 HINT2 84681
Proximity Label-MS Homo sapiens
309 AHNAK2 113146
Cross-Linking-MS (XL-MS) Homo sapiens
310 SF3A2 8175
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
311 ITGA4 3676
Affinity Capture-MS Homo sapiens
312 ATG16L1 55054
Affinity Capture-MS Homo sapiens
313 HSPA6 3310
Co-fractionation Homo sapiens
314 TFCP2 7024
Affinity Capture-MS Homo sapiens
315 MRPL4 51073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
316 SERBP1 26135
Affinity Capture-MS Homo sapiens
317 YAF2  
Affinity Capture-MS Homo sapiens
318 MTERF3  
Proximity Label-MS Homo sapiens
319 ALOXE3  
Affinity Capture-MS Homo sapiens
320 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
321 MDH2 4191
Proximity Label-MS Homo sapiens
322 UBR4 23352
Co-fractionation Homo sapiens
323 CDKN2AIP  
Affinity Capture-MS Homo sapiens
324 ATP5A1 498
Co-fractionation Homo sapiens
325 LMNA 4000
Affinity Capture-MS Homo sapiens
326 NME2 4831
Cross-Linking-MS (XL-MS) Homo sapiens
327 AKAP12 9590
Cross-Linking-MS (XL-MS) Homo sapiens
328 ACSS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
329 FASTKD5  
Proximity Label-MS Homo sapiens
330 GLYATL1  
Affinity Capture-MS Homo sapiens
331 B4GALT2  
Affinity Capture-MS Homo sapiens
332 RALA 5898
Cross-Linking-MS (XL-MS) Homo sapiens
333 CBX3 11335
Co-fractionation Homo sapiens
334 ACACA 31
Co-fractionation Homo sapiens
335 ABCF2 10061
Cross-Linking-MS (XL-MS) Homo sapiens
336 CHMP4B 128866
Affinity Capture-MS Homo sapiens
337 RPA2 6118
Proximity Label-MS Homo sapiens
338 GID8 54994
Co-fractionation Homo sapiens
339 NTRK1 4914
Affinity Capture-MS Homo sapiens
340 GLI3 2737
Affinity Capture-MS Homo sapiens
341 U2AF2 11338
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
342 ARNT 405
Affinity Capture-MS Homo sapiens
343 NARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
344 DCAF4L1  
Affinity Capture-MS Homo sapiens
345 SMS 6611
Co-fractionation Homo sapiens
346 SUB1 10923
Cross-Linking-MS (XL-MS) Homo sapiens
347 RAB10 10890
Co-localization Homo sapiens
348 HDAC5 10014
Affinity Capture-MS Homo sapiens
349 TMEM70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
350 SF3A3 10946
Co-fractionation Homo sapiens
351 TRMT11 60487
Affinity Capture-MS Homo sapiens
352 PCBP2 5094
Cross-Linking-MS (XL-MS) Homo sapiens
353 SLX4  
Affinity Capture-MS Homo sapiens
354 GLOD4 51031
Co-fractionation Homo sapiens
355 NUDT9 53343
Cross-Linking-MS (XL-MS) Homo sapiens
356 CDC73  
Affinity Capture-MS Homo sapiens
357 CHERP 10523
Co-fractionation Homo sapiens
358 BAZ1B 9031
Cross-Linking-MS (XL-MS) Homo sapiens
359 ECT2 1894
Affinity Capture-MS Homo sapiens
360 EEF2 1938
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
361 METTL20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
362 NR3C1 2908
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
363 DDB2  
Affinity Capture-MS Homo sapiens
364 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
365 METTL21B  
Affinity Capture-MS Homo sapiens
366 CECR5 27440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
367 INPP5K 51763
Affinity Capture-MS Homo sapiens
368 HNRNPAB 3182
Co-fractionation Homo sapiens
369 ABTB2 25841
Affinity Capture-MS Homo sapiens
370 HEATR5B 54497
Cross-Linking-MS (XL-MS) Homo sapiens
371 EMC2 9694
Cross-Linking-MS (XL-MS) Homo sapiens
372 RUNX2  
Affinity Capture-MS Homo sapiens
373 TFAP2D  
Affinity Capture-MS Homo sapiens
374 RAF1 5894
Affinity Capture-MS Homo sapiens
375 HSPD1 3329
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
376 YBX1 4904
Co-fractionation Homo sapiens
377 HSPA9 3313
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
378 PUS1 80324
Affinity Capture-MS Homo sapiens
379 NEUROG3  
Affinity Capture-MS Homo sapiens
380 CA2 760
Co-crystal Structure Homo sapiens
381 ILF3 3609
Co-fractionation Homo sapiens
382 RBM17 84991
Co-fractionation Homo sapiens
383 NDUFS2 4720
Affinity Capture-MS Homo sapiens
384 AHSA1 10598
Affinity Capture-MS Homo sapiens
385 SLC25A43  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
386 LAMB1 3912
Co-fractionation Homo sapiens
387 PRMT3 10196
Co-fractionation Homo sapiens
388 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
389 TSFM 10102
Proximity Label-MS Homo sapiens
390 PDK3 5165
Affinity Capture-MS Homo sapiens
391 ICT1 3396
Affinity Capture-MS Homo sapiens
392 PRRC2C 23215
Co-fractionation Homo sapiens
393 PDHA1 5160
Proximity Label-MS Homo sapiens
394 RASL10B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
395 ABL1 25
Affinity Capture-Western Homo sapiens
396 HSPB1 3315
Co-fractionation Homo sapiens
397 PLOD1 5351
Co-fractionation Homo sapiens
398 GNB1 2782
Co-fractionation Homo sapiens
399 NSUN3  
Affinity Capture-MS Homo sapiens
400 TRAP1 10131
Co-fractionation Homo sapiens
401 UFL1 23376
Affinity Capture-MS Homo sapiens
402 BAK1 578
Affinity Capture-Western Homo sapiens
403 TBRG4 9238
Proximity Label-MS Homo sapiens
404 AARS2  
Proximity Label-MS Homo sapiens
405 LRRK2 120892
Biochemical Activity Homo sapiens
406 RPUSD3  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
407 METTL3  
Affinity Capture-MS Homo sapiens
408 TAF1C  
Affinity Capture-MS Homo sapiens
409 ERG  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
410 AR 367
Affinity Capture-MS Homo sapiens
411 TMCC2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
412 RC3H1 149041
Affinity Capture-MS Homo sapiens
413 ATXN3 4287
Affinity Capture-MS Homo sapiens
414 PRDX3 10935
Co-fractionation Homo sapiens
415 BCR 613
Affinity Capture-Western Homo sapiens
416 CTSH 1512
Affinity Capture-MS Homo sapiens
417 UBE2H 7328
Affinity Capture-MS Homo sapiens
418 INSIG1  
Affinity Capture-MS Homo sapiens
419 SLFN11 91607
Affinity Capture-MS Homo sapiens
420 RIT1 6016
Negative Genetic Homo sapiens
421 PCED1A  
Affinity Capture-MS Homo sapiens
422 LGR4 55366
Affinity Capture-MS Homo sapiens
423 SMURF1 57154
Affinity Capture-MS Homo sapiens
424 PPIE 10450
Affinity Capture-MS Homo sapiens
425 NOTCH2NL 388677
Two-hybrid Homo sapiens
426 TRUB2  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
427 MRPL37 51253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
428 KRTAP5-9  
Two-hybrid Homo sapiens
429 RPUSD4 84881
Proximity Label-MS Homo sapiens
430 SNRPD2 6633
Co-fractionation Homo sapiens
431 APP 351
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
432 Cdkn2aip  
Affinity Capture-MS Mus musculus
433 HDAC1 3065
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
434 KIAA1429 25962
Affinity Capture-MS Homo sapiens
435 GP5 2814
Affinity Capture-MS Homo sapiens
436 SSC4D  
Affinity Capture-MS Homo sapiens
437 PRC1 9055
Affinity Capture-MS Homo sapiens
438 CEP70  
Two-hybrid Homo sapiens
439 TGFB1 7040
Affinity Capture-MS Homo sapiens
440 PCK1 5105
Affinity Capture-MS Homo sapiens
441 COPS5 10987
Affinity Capture-MS Homo sapiens
442 MRPS24 64951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
443 STK19  
Affinity Capture-MS Homo sapiens
444 GCAT  
Affinity Capture-MS Homo sapiens
445 FOXD4L6  
Affinity Capture-MS Homo sapiens
446 ALK 238
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
447 EXD2  
Proximity Label-MS Homo sapiens
448 ASTL  
Affinity Capture-MS Homo sapiens
449 EED  
Affinity Capture-MS Homo sapiens
450 BCOR  
Affinity Capture-MS Homo sapiens
451 IGFN1  
Affinity Capture-MS Homo sapiens
452 METTL14  
Affinity Capture-MS Homo sapiens
453 FAHD2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
454 C6orf15  
Affinity Capture-MS Homo sapiens
455 TULP2  
Affinity Capture-MS Homo sapiens
456 MYCN  
Affinity Capture-MS Homo sapiens
457 ACSF2 80221
Affinity Capture-MS Homo sapiens
458 ADSS 159
Affinity Capture-MS Homo sapiens
459 FAM20C 56975
Affinity Capture-MS Homo sapiens
460 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
461 DVL2 1856
Affinity Capture-MS Homo sapiens
462 SLC25A33  
Co-fractionation Homo sapiens
463 RAB1B 81876
Co-fractionation Homo sapiens
464 MRPS30 10884
Affinity Capture-MS Homo sapiens
465 PRDM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
466 ACSM5 54988
Affinity Capture-MS Homo sapiens
467 PCK2 5106
Affinity Capture-MS Homo sapiens
468 RAB5B 5869
Co-fractionation Homo sapiens
469 CCAR2 57805
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
470 STAU1 6780
Affinity Capture-MS Homo sapiens
471 ATF2  
Affinity Capture-MS Homo sapiens
472 HSCB 150274
Proximity Label-MS Homo sapiens
473 VCP 7415
Affinity Capture-MS Homo sapiens
474 CYSRT1 375791
Two-hybrid Homo sapiens
475 C11orf1  
Affinity Capture-MS Homo sapiens
476 IFI16 3428
Affinity Capture-MS Homo sapiens
477 ASB4  
Affinity Capture-MS Homo sapiens
478 AHNAK 79026
Co-fractionation Homo sapiens
479 SF3B6 51639
Co-fractionation Homo sapiens
480 C12orf10 60314
Co-fractionation Homo sapiens
481 CRY2  
Affinity Capture-MS Homo sapiens
482 PML 5371
Affinity Capture-MS Homo sapiens
483 LYRM4 57128
Co-fractionation Homo sapiens
484 IDH3G 3421
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
485 BRD4 23476
Cross-Linking-MS (XL-MS) Homo sapiens
486 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
487 ATP5B 506
Co-fractionation Homo sapiens
488 DNAJB11 51726
Co-fractionation Homo sapiens
489 DKK3 27122
Affinity Capture-MS Homo sapiens
490 PHB 5245
Co-fractionation Homo sapiens
491 GAN 8139
Affinity Capture-MS Homo sapiens
492 MRRF  
Proximity Label-MS Homo sapiens
493 FANCD2  
Affinity Capture-MS Homo sapiens
494 ASS1 445
Affinity Capture-MS Homo sapiens
495 TRIM31  
Affinity Capture-MS Homo sapiens
496 AMBRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
497 NDUFA9 4704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
498 SNRPB 6628
Co-fractionation Homo sapiens
499 ITLN2  
Affinity Capture-MS Homo sapiens
500 CTNNB1 1499
Affinity Capture-MS Homo sapiens
501 SRSF1 6426
Co-fractionation Homo sapiens
502 SAP30BP  
Co-fractionation Homo sapiens
503 TXNRD1 7296
Co-fractionation Homo sapiens
504 PDIA3 2923
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
505 SARNP 84324
Co-fractionation Homo sapiens
506 PRAMEF17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
507 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
508 NXF1 10482
Affinity Capture-RNA Homo sapiens
509 SF3A1 10291
Co-fractionation Homo sapiens
510 AURKB 9212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
511 LZTS2 84445
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
512 PRKACA 5566
Co-fractionation Homo sapiens
513 MAPK6  
Two-hybrid Homo sapiens
514 FBXO6 26270
Affinity Capture-MS Homo sapiens
515 ODF3L1  
Affinity Capture-MS Homo sapiens
516 ATP6V1A 523
Co-fractionation Homo sapiens
517 DAZL  
Affinity Capture-MS Homo sapiens
518 KRTAP10-3  
Two-hybrid Homo sapiens
519 C1orf94  
Affinity Capture-MS Homo sapiens
520 SIRT2 22933
Affinity Capture-MS Homo sapiens
521 FBXO15 201456
Affinity Capture-MS Homo sapiens
522 QTRT1 81890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
523 SF3B3 23450
Co-fractionation Homo sapiens
524 VDAC1 7416
Co-fractionation Homo sapiens
525 SF3B2 10992
Co-fractionation Homo sapiens
526 CCDC8  
Affinity Capture-MS Homo sapiens
527 DHX15 1665
Co-fractionation Homo sapiens
528 NME6  
Affinity Capture-MS Homo sapiens
529 Ska1  
Affinity Capture-MS Mus musculus
530 SDR39U1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
531 C11orf58  
Co-fractionation Homo sapiens
532 TUBG1 7283
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
533 ABHD10 55347
Affinity Capture-MS Homo sapiens
534 RPAP1  
Affinity Capture-MS Homo sapiens
535 HNRNPA2B1 3181
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
536 SMNDC1  
Co-fractionation Homo sapiens
537 Ckap5 75786
Affinity Capture-MS Mus musculus
538 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
539 ELAVL1 1994
Co-fractionation Homo sapiens
540 GBA 2629
Affinity Capture-MS Homo sapiens
541 C21orf33  
Proximity Label-MS Homo sapiens
542 OGDHL  
Affinity Capture-MS Homo sapiens
543 TXN 7295
Co-fractionation Homo sapiens
544 C9orf173  
Affinity Capture-MS Homo sapiens
545 MOV10 4343
Affinity Capture-RNA Homo sapiens
546 CDK9 1025
Affinity Capture-MS Homo sapiens
547 PINK1  
Affinity Capture-MS Homo sapiens
548 XRCC6 2547
Proximity Label-MS Homo sapiens
549 EIF5A 1984
Co-fractionation Homo sapiens
550 OXCT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
551 RARS 5917
Co-fractionation Homo sapiens
552 C9orf72  
Affinity Capture-MS Homo sapiens
553 METTL17  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
554 SCARB2 950
Co-fractionation Homo sapiens
555 CNDP2 55748
Co-fractionation Homo sapiens
556 DCAF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
557 NEDD8 4738
Affinity Capture-MS Homo sapiens
558 ALDOA 226
Co-fractionation Homo sapiens
559 CDC5L 988
Affinity Capture-MS Homo sapiens
560 PIK3R3 8503
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here