Gene ontology annotations for NHP2L1
Experiment description of studies that identified NHP2L1 in sEVs
1
Experiment ID
489
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
412
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MCF7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
6
Experiment ID
414
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
7
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
10
Experiment ID
498
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
11
Experiment ID
407
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
12
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
13
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
14
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
15
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
16
Experiment ID
405
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Foreskin fibroblasts
Sample name
BJ
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
17
Experiment ID
237
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
18
Experiment ID
417
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Lymphoma cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
19
Experiment ID
411
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Mammary cancer-associated fibroblasts
Sample name
mCAF
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
20
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
21
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
22
Experiment ID
413
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Normal mammary epithelial cells
Sample name
MCF10A
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
23
Experiment ID
406
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
BxPC3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
24
Experiment ID
415
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
25
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
26
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
27
Experiment ID
408
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPDE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
28
Experiment ID
409
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPNE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
29
Experiment ID
416
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pluripotent stem cells
Sample name
PSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
30
Experiment ID
191
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
31
Experiment ID
410
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
T lymphocytes
Sample name
Jurkat
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
32
Experiment ID
217
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for NHP2L1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
UBE2H
7328
Affinity Capture-MS
Homo sapiens
2
RIT1
6016
Negative Genetic
Homo sapiens
3
RPSA
3921
Co-fractionation
Homo sapiens
4
HNRNPC
3183
Co-fractionation
Homo sapiens
5
NOP58
51602
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
6
MCM2
4171
Affinity Capture-MS
Homo sapiens
7
DPH2
1802
Co-fractionation
Homo sapiens
8
Prpf8
192159
Affinity Capture-MS
Mus musculus
9
ANLN
54443
Affinity Capture-MS
Homo sapiens
10
EML2
24139
Co-fractionation
Homo sapiens
11
RPS4X
6191
Co-fractionation
Homo sapiens
12
RPL30
6156
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
13
PSMD11
5717
Co-fractionation
Homo sapiens
14
BTF3
689
Affinity Capture-MS
Homo sapiens
15
RPL37A
6168
Co-fractionation
Homo sapiens
16
NOP56
10528
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
17
NOLC1
9221
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
18
SURF4
6836
Co-fractionation
Homo sapiens
19
RPL15
6138
Co-fractionation
Homo sapiens
20
SSB
6741
Affinity Capture-MS
Homo sapiens
21
PRPF3
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
22
RPL12
6136
Co-fractionation
Homo sapiens
23
CAND1
55832
Affinity Capture-MS
Homo sapiens
24
HNRNPR
10236
Co-fractionation
Homo sapiens
25
PRPF40A
55660
Co-fractionation
Homo sapiens
26
SNRPF
6636
Affinity Capture-MS
Homo sapiens
27
SNRPD2
6633
Affinity Capture-MS
Homo sapiens
28
RPS3
6188
Co-fractionation
Homo sapiens
29
USP36
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
30
MAP1LC3B
81631
Two-hybrid
Homo sapiens
31
RPL4
6124
Co-fractionation
Homo sapiens
32
RUVBL1
8607
Affinity Capture-Western
Homo sapiens
33
RNPS1
10921
Co-fractionation
Homo sapiens
34
CORO1B
57175
Co-fractionation
Homo sapiens
35
ROCK2
9475
Co-fractionation
Homo sapiens
36
IMPDH2
3615
Co-fractionation
Homo sapiens
37
RBM39
9584
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
38
KIF23
9493
Affinity Capture-MS
Homo sapiens
39
DDX24
57062
Co-fractionation
Homo sapiens
40
TBL1XR1
79718
Co-fractionation
Homo sapiens
41
SRSF10
10772
Co-fractionation
Homo sapiens
42
PRC1
9055
Affinity Capture-MS
Homo sapiens
43
CALD1
800
Affinity Capture-MS
Homo sapiens
44
SNRPC
6631
Affinity Capture-MS
Homo sapiens
45
RPS8
6202
Co-fractionation
Homo sapiens
46
FBL
2091
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
47
SAP18
10284
Co-fractionation
Homo sapiens
48
SRP14
6727
Co-fractionation
Homo sapiens
49
PCDHA2
Co-fractionation
Homo sapiens
50
ITGA4
3676
Affinity Capture-MS
Homo sapiens
51
EED
Affinity Capture-MS
Homo sapiens
52
U2AF1
7307
Co-fractionation
Homo sapiens
53
DDX18
8886
Co-fractionation
Homo sapiens
54
VCAM1
7412
Affinity Capture-MS
Homo sapiens
55
CTPS1
1503
Co-fractionation
Homo sapiens
56
MYCN
Affinity Capture-MS
Homo sapiens
57
RBM42
Affinity Capture-MS
Homo sapiens
58
RPS11
6205
Co-fractionation
Homo sapiens
59
PABPC4
8761
Co-fractionation
Homo sapiens
60
EEF1A1
1915
Co-fractionation
Homo sapiens
61
USP37
Proximity Label-MS
Homo sapiens
62
KIF14
9928
Affinity Capture-MS
Homo sapiens
63
KIAA0196
9897
Co-fractionation
Homo sapiens
64
PES1
23481
Co-fractionation
Homo sapiens
65
LIN28A
Affinity Capture-MS
Homo sapiens
66
IMPDH1
3614
Co-fractionation
Homo sapiens
67
VTN
7448
Co-fractionation
Homo sapiens
68
CHMP4C
92421
Affinity Capture-MS
Homo sapiens
69
AURKA
6790
Affinity Capture-MS
Homo sapiens
70
RFXAP
Co-fractionation
Homo sapiens
71
PRPF6
24148
Co-fractionation
Homo sapiens
72
DLD
1738
Affinity Capture-MS
Homo sapiens
73
LMNB1
4001
Affinity Capture-MS
Homo sapiens
74
FOXA1
Affinity Capture-MS
Homo sapiens
75
UTP14A
10813
Co-fractionation
Homo sapiens
76
HIST1H4A
8359
Affinity Capture-MS
Homo sapiens
77
PPIH
10465
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
78
NAA50
80218
Co-fractionation
Homo sapiens
79
CLVS2
134829
Co-fractionation
Homo sapiens
80
CUL4B
8450
Co-fractionation
Homo sapiens
81
MEPCE
56257
Affinity Capture-MS
Homo sapiens
82
BCAS2
10286
Co-fractionation
Homo sapiens
83
RRP9
9136
Affinity Capture-MS
Homo sapiens
84
GLDC
2731
Co-fractionation
Homo sapiens
85
TRA2A
29896
Co-fractionation
Homo sapiens
86
EIF2A
83939
Co-fractionation
Homo sapiens
87
MAPRE1
22919
Affinity Capture-MS
Homo sapiens
88
LRIF1
Two-hybrid
Homo sapiens
89
POLA2
Two-hybrid
Homo sapiens
90
ACOT13
Co-fractionation
Homo sapiens
91
RPL5
6125
Co-fractionation
Homo sapiens
92
GCC2
9648
Co-fractionation
Homo sapiens
93
RPL18A
6142
Co-fractionation
Homo sapiens
94
PABPC1
26986
Co-fractionation
Homo sapiens
95
CHMP4B
128866
Affinity Capture-MS
Homo sapiens
96
RPA2
6118
Proximity Label-MS
Homo sapiens
97
CDKN2C
Two-hybrid
Homo sapiens
98
RPL31
6160
Co-fractionation
Homo sapiens
99
RPS7
6201
Co-fractionation
Homo sapiens
100
KDM4B
Co-fractionation
Homo sapiens
101
RAD18
Affinity Capture-MS
Homo sapiens
102
SYNCRIP
10492
Co-fractionation
Homo sapiens
103
TPBG
7162
Co-fractionation
Homo sapiens
104
SNRPN
6638
Affinity Capture-MS
Homo sapiens
105
Nhp2l1
20826
Affinity Capture-MS
Mus musculus
106
HECTD1
25831
Affinity Capture-MS
Homo sapiens
107
RPLP0
6175
Co-fractionation
Homo sapiens
108
SRPK3
Affinity Capture-MS
Homo sapiens
109
SON
6651
Co-fractionation
Homo sapiens
110
PRPF31
26121
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
111
STAG2
10735
Co-fractionation
Homo sapiens
112
METTL1
4234
Co-fractionation
Homo sapiens
113
TRA2B
6434
Co-fractionation
Homo sapiens
114
RBM10
Two-hybrid
Homo sapiens
115
RPL11
6135
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
116
RSL1D1
26156
Co-fractionation
Homo sapiens
117
ZNF326
284695
Co-fractionation
Homo sapiens
118
CHST1
8534
Two-hybrid
Homo sapiens
119
SRSF11
9295
Co-fractionation
Homo sapiens
120
ZBTB2
57621
Affinity Capture-MS
Homo sapiens
121
RHOU
58480
Reconstituted Complex
Homo sapiens
122
RPL23
9349
Co-fractionation
Homo sapiens
123
MRTO4
51154
Co-fractionation
Homo sapiens
124
RPS2
6187
Co-fractionation
Homo sapiens
125
METTL16
79066
Co-fractionation
Homo sapiens
126
EIF6
3692
Co-fractionation
Homo sapiens
127
RC3H2
Affinity Capture-MS
Homo sapiens
128
ECT2
1894
Affinity Capture-MS
Homo sapiens
129
SNRPB
6628
Affinity Capture-MS
Homo sapiens
130
GTF3C1
Two-hybrid
Homo sapiens
131
RPS6
6194
Co-fractionation
Homo sapiens
132
DCAF13
25879
Affinity Capture-MS
Homo sapiens
133
SRSF5
6430
Co-fractionation
Homo sapiens
134
EGFR
1956
Negative Genetic
Homo sapiens
135
HSD17B10
3028
Affinity Capture-MS
Homo sapiens
136
YY1
7528
Co-fractionation
Homo sapiens
137
TRMT6
51605
Co-fractionation
Homo sapiens
138
EPRS
2058
Cross-Linking-MS (XL-MS)
Homo sapiens
139
RPL7
6129
Co-fractionation
Homo sapiens
140
POLR2A
5430
Affinity Capture-MS
Homo sapiens
141
SUZ12
Affinity Capture-MS
Homo sapiens
142
SART1
9092
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
143
FN1
2335
Affinity Capture-MS
Homo sapiens
144
PUS7
54517
Co-fractionation
Homo sapiens
145
RRP7A
27341
Co-fractionation
Homo sapiens
146
CDK2
1017
Affinity Capture-MS
Homo sapiens
147
ZW10
9183
Co-fractionation
Homo sapiens
148
SSR3
6747
Co-fractionation
Homo sapiens
149
DKC1
1736
Co-fractionation
Homo sapiens
150
MYC
Affinity Capture-MS
Homo sapiens
151
RPA3
6119
Proximity Label-MS
Homo sapiens
152
LSM3
27258
Affinity Capture-MS
Homo sapiens
153
RPS13
6207
Co-fractionation
Homo sapiens
154
ZC3H18
Co-fractionation
Homo sapiens
155
DLST
1743
Affinity Capture-MS
Homo sapiens
156
RPS14
6208
Co-fractionation
Homo sapiens
157
TOP1
7150
Affinity Capture-MS
Homo sapiens
158
NHP2L1
4809
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
159
SIRT6
Affinity Capture-MS
Homo sapiens
160
SART3
9733
Affinity Capture-MS
Homo sapiens
161
CORO1A
11151
Co-fractionation
Homo sapiens
162
DDB1
1642
Co-fractionation
Homo sapiens
163
RPL23A
6147
Co-fractionation
Homo sapiens
164
RPL18
6141
Co-fractionation
Homo sapiens
165
RNF2
Affinity Capture-MS
Homo sapiens
166
BMI1
Affinity Capture-MS
Homo sapiens
167
TPR
7175
Co-fractionation
Homo sapiens
168
KIAA0368
23392
Co-fractionation
Homo sapiens
169
NOP16
51491
Co-fractionation
Homo sapiens
170
PABPC3
5042
Co-fractionation
Homo sapiens
171
RPS15A
6210
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
172
CORO1C
23603
Co-fractionation
Homo sapiens
173
SUPT16H
11198
Co-fractionation
Homo sapiens
174
GNL3
26354
Co-fractionation
Homo sapiens
175
RPL19
6143
Co-fractionation
Homo sapiens
176
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
177
HSPB2
Two-hybrid
Homo sapiens
178
CPSF6
11052
Affinity Capture-MS
Homo sapiens
179
RAD17
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
180
RPL6
6128
Co-fractionation
Homo sapiens
181
XRCC6
2547
Two-hybrid
Homo sapiens
182
RPLP0P6
220717
Co-fractionation
Homo sapiens
183
SF3B4
10262
Co-fractionation
Homo sapiens
184
MEN1
4221
Affinity Capture-MS
Homo sapiens
185
RPAP1
Co-fractionation
Homo sapiens
186
BOP1
23246
Co-fractionation
Homo sapiens
187
SNRPA
6626
Affinity Capture-MS
Homo sapiens
188
HNRNPM
4670
Co-fractionation
Homo sapiens
189
SMURF1
57154
Affinity Capture-MS
Homo sapiens
190
LSM5
Affinity Capture-MS
Homo sapiens
191
GTPBP1
9567
Co-fractionation
Homo sapiens
192
RPS5
6193
Co-fractionation
Homo sapiens
193
TARS
6897
Co-fractionation
Homo sapiens
194
NIFK
84365
Co-fractionation
Homo sapiens
195
HNRNPK
3190
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
196
WDR18
57418
Co-fractionation
Homo sapiens
197
Prpf3
70767
Affinity Capture-MS
Mus musculus
198
PCNA
5111
Co-fractionation
Homo sapiens
199
TRIM55
Co-fractionation
Homo sapiens
200
LSM7
Affinity Capture-MS
Homo sapiens
201
RPS24
6229
Co-fractionation
Homo sapiens
202
CDV3
55573
Co-fractionation
Homo sapiens
203
HNRNPL
3191
Co-fractionation
Homo sapiens
204
RPS23
6228
Co-fractionation
Homo sapiens
205
SRSF7
6432
Co-fractionation
Homo sapiens
206
NUFIP1
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
207
RAB11A
8766
Affinity Capture-MS
Homo sapiens
208
CIT
11113
Affinity Capture-MS
Homo sapiens
209
SERBP1
26135
Affinity Capture-MS
Homo sapiens
210
LSM6
11157
Affinity Capture-MS
Homo sapiens
211
PRPF4
9128
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
212
SNW1
22938
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
213
HNRNPU
3192
Co-fractionation
Homo sapiens
214
RC3H1
149041
Affinity Capture-MS
Homo sapiens
215
PTRF
284119
Co-fractionation
Homo sapiens
216
MATN2
4147
Affinity Capture-MS
Homo sapiens
217
DDX56
Co-fractionation
Homo sapiens
218
RRS1
23212
Co-fractionation
Homo sapiens
219
ZNHIT3
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
View the network
image/svg+xml
Pathways in which NHP2L1 is involved