Gene description for NHP2L1
Gene name NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)
Gene symbol NHP2L1
Other names/aliases 15.5K
FA-1
FA1
NHPX
OTK27
SNRNP15-5
SNU13
SPAG12
SSFA1
Species Homo sapiens
 Database cross references - NHP2L1
ExoCarta ExoCarta_4809
Vesiclepedia VP_4809
Entrez Gene 4809
HGNC 7819
MIM 601304
UniProt P55769  
 NHP2L1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Hepatocytes 26054723    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for NHP2L1
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
    snoRNA binding GO:0030515 IDA
    U4 snRNA binding GO:0030621 IDA
    U4atac snRNA binding GO:0030622 IDA
    U3 snoRNA binding GO:0034511 IDA
    box C/D sno(s)RNA binding GO:0034512 IDA
    ATPase binding GO:0051117 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 NAS
    maturation of SSU-rRNA GO:0030490 IBA
    ribosomal small subunit biogenesis GO:0042274 IDA
Subcellular Localization
    dense fibrillar component GO:0001651 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    U4atac snRNP GO:0005690 IDA
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    box C/D methylation guide snoRNP complex GO:0031428 IBA
    box C/D methylation guide snoRNP complex GO:0031428 IDA
    box C/D methylation guide snoRNP complex GO:0031428 NAS
    small-subunit processome GO:0032040 IBA
    small-subunit processome GO:0032040 IDA
    protein-containing complex GO:0032991 IDA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IDA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IPI
    U2-type precatalytic spliceosome GO:0071005 IDA
    precatalytic spliceosome GO:0071011 IBA
 Experiment description of studies that identified NHP2L1 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 407
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 405
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 237
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
18
Experiment ID 417
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 411
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 413
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 406
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 415
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 408
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 409
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 416
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
31
Experiment ID 410
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NHP2L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 RPSA 3921
Co-fractionation Homo sapiens
4 HNRNPC 3183
Co-fractionation Homo sapiens
5 NOP58 51602
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
6 MCM2 4171
Affinity Capture-MS Homo sapiens
7 DPH2 1802
Co-fractionation Homo sapiens
8 Prpf8 192159
Affinity Capture-MS Mus musculus
9 ANLN 54443
Affinity Capture-MS Homo sapiens
10 EML2 24139
Co-fractionation Homo sapiens
11 RPS4X 6191
Co-fractionation Homo sapiens
12 RPL30 6156
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
13 PSMD11 5717
Co-fractionation Homo sapiens
14 BTF3 689
Affinity Capture-MS Homo sapiens
15 RPL37A 6168
Co-fractionation Homo sapiens
16 NOP56 10528
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
17 NOLC1 9221
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
18 SURF4 6836
Co-fractionation Homo sapiens
19 RPL15 6138
Co-fractionation Homo sapiens
20 SSB 6741
Affinity Capture-MS Homo sapiens
21 PRPF3  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
22 RPL12 6136
Co-fractionation Homo sapiens
23 CAND1 55832
Affinity Capture-MS Homo sapiens
24 HNRNPR 10236
Co-fractionation Homo sapiens
25 PRPF40A 55660
Co-fractionation Homo sapiens
26 SNRPF 6636
Affinity Capture-MS Homo sapiens
27 SNRPD2 6633
Affinity Capture-MS Homo sapiens
28 RPS3 6188
Co-fractionation Homo sapiens
29 USP36  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
30 MAP1LC3B 81631
Two-hybrid Homo sapiens
31 RPL4 6124
Co-fractionation Homo sapiens
32 RUVBL1 8607
Affinity Capture-Western Homo sapiens
33 RNPS1 10921
Co-fractionation Homo sapiens
34 CORO1B 57175
Co-fractionation Homo sapiens
35 ROCK2 9475
Co-fractionation Homo sapiens
36 IMPDH2 3615
Co-fractionation Homo sapiens
37 RBM39 9584
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
38 KIF23 9493
Affinity Capture-MS Homo sapiens
39 DDX24 57062
Co-fractionation Homo sapiens
40 TBL1XR1 79718
Co-fractionation Homo sapiens
41 SRSF10 10772
Co-fractionation Homo sapiens
42 PRC1 9055
Affinity Capture-MS Homo sapiens
43 CALD1 800
Affinity Capture-MS Homo sapiens
44 SNRPC 6631
Affinity Capture-MS Homo sapiens
45 RPS8 6202
Co-fractionation Homo sapiens
46 FBL 2091
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
47 SAP18 10284
Co-fractionation Homo sapiens
48 SRP14 6727
Co-fractionation Homo sapiens
49 PCDHA2  
Co-fractionation Homo sapiens
50 ITGA4 3676
Affinity Capture-MS Homo sapiens
51 EED  
Affinity Capture-MS Homo sapiens
52 U2AF1 7307
Co-fractionation Homo sapiens
53 DDX18 8886
Co-fractionation Homo sapiens
54 VCAM1 7412
Affinity Capture-MS Homo sapiens
55 CTPS1 1503
Co-fractionation Homo sapiens
56 MYCN  
Affinity Capture-MS Homo sapiens
57 RBM42  
Affinity Capture-MS Homo sapiens
58 RPS11 6205
Co-fractionation Homo sapiens
59 PABPC4 8761
Co-fractionation Homo sapiens
60 EEF1A1 1915
Co-fractionation Homo sapiens
61 USP37  
Proximity Label-MS Homo sapiens
62 KIF14 9928
Affinity Capture-MS Homo sapiens
63 KIAA0196 9897
Co-fractionation Homo sapiens
64 PES1 23481
Co-fractionation Homo sapiens
65 LIN28A  
Affinity Capture-MS Homo sapiens
66 IMPDH1 3614
Co-fractionation Homo sapiens
67 VTN 7448
Co-fractionation Homo sapiens
68 CHMP4C 92421
Affinity Capture-MS Homo sapiens
69 AURKA 6790
Affinity Capture-MS Homo sapiens
70 RFXAP  
Co-fractionation Homo sapiens
71 PRPF6 24148
Co-fractionation Homo sapiens
72 DLD 1738
Affinity Capture-MS Homo sapiens
73 LMNB1 4001
Affinity Capture-MS Homo sapiens
74 FOXA1  
Affinity Capture-MS Homo sapiens
75 UTP14A 10813
Co-fractionation Homo sapiens
76 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
77 PPIH 10465
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
78 NAA50 80218
Co-fractionation Homo sapiens
79 CLVS2 134829
Co-fractionation Homo sapiens
80 CUL4B 8450
Co-fractionation Homo sapiens
81 MEPCE 56257
Affinity Capture-MS Homo sapiens
82 BCAS2 10286
Co-fractionation Homo sapiens
83 RRP9 9136
Affinity Capture-MS Homo sapiens
84 GLDC 2731
Co-fractionation Homo sapiens
85 TRA2A 29896
Co-fractionation Homo sapiens
86 EIF2A 83939
Co-fractionation Homo sapiens
87 MAPRE1 22919
Affinity Capture-MS Homo sapiens
88 LRIF1  
Two-hybrid Homo sapiens
89 POLA2  
Two-hybrid Homo sapiens
90 ACOT13  
Co-fractionation Homo sapiens
91 RPL5 6125
Co-fractionation Homo sapiens
92 GCC2 9648
Co-fractionation Homo sapiens
93 RPL18A 6142
Co-fractionation Homo sapiens
94 PABPC1 26986
Co-fractionation Homo sapiens
95 CHMP4B 128866
Affinity Capture-MS Homo sapiens
96 RPA2 6118
Proximity Label-MS Homo sapiens
97 CDKN2C  
Two-hybrid Homo sapiens
98 RPL31 6160
Co-fractionation Homo sapiens
99 RPS7 6201
Co-fractionation Homo sapiens
100 KDM4B  
Co-fractionation Homo sapiens
101 RAD18  
Affinity Capture-MS Homo sapiens
102 SYNCRIP 10492
Co-fractionation Homo sapiens
103 TPBG 7162
Co-fractionation Homo sapiens
104 SNRPN 6638
Affinity Capture-MS Homo sapiens
105 Nhp2l1 20826
Affinity Capture-MS Mus musculus
106 HECTD1 25831
Affinity Capture-MS Homo sapiens
107 RPLP0 6175
Co-fractionation Homo sapiens
108 SRPK3  
Affinity Capture-MS Homo sapiens
109 SON 6651
Co-fractionation Homo sapiens
110 PRPF31 26121
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
111 STAG2 10735
Co-fractionation Homo sapiens
112 METTL1 4234
Co-fractionation Homo sapiens
113 TRA2B 6434
Co-fractionation Homo sapiens
114 RBM10  
Two-hybrid Homo sapiens
115 RPL11 6135
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
116 RSL1D1 26156
Co-fractionation Homo sapiens
117 ZNF326 284695
Co-fractionation Homo sapiens
118 CHST1 8534
Two-hybrid Homo sapiens
119 SRSF11 9295
Co-fractionation Homo sapiens
120 ZBTB2 57621
Affinity Capture-MS Homo sapiens
121 RHOU 58480
Reconstituted Complex Homo sapiens
122 RPL23 9349
Co-fractionation Homo sapiens
123 MRTO4 51154
Co-fractionation Homo sapiens
124 RPS2 6187
Co-fractionation Homo sapiens
125 METTL16 79066
Co-fractionation Homo sapiens
126 EIF6 3692
Co-fractionation Homo sapiens
127 RC3H2  
Affinity Capture-MS Homo sapiens
128 ECT2 1894
Affinity Capture-MS Homo sapiens
129 SNRPB 6628
Affinity Capture-MS Homo sapiens
130 GTF3C1  
Two-hybrid Homo sapiens
131 RPS6 6194
Co-fractionation Homo sapiens
132 DCAF13 25879
Affinity Capture-MS Homo sapiens
133 SRSF5 6430
Co-fractionation Homo sapiens
134 EGFR 1956
Negative Genetic Homo sapiens
135 HSD17B10 3028
Affinity Capture-MS Homo sapiens
136 YY1 7528
Co-fractionation Homo sapiens
137 TRMT6 51605
Co-fractionation Homo sapiens
138 EPRS 2058
Cross-Linking-MS (XL-MS) Homo sapiens
139 RPL7 6129
Co-fractionation Homo sapiens
140 POLR2A 5430
Affinity Capture-MS Homo sapiens
141 SUZ12  
Affinity Capture-MS Homo sapiens
142 SART1 9092
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
143 FN1 2335
Affinity Capture-MS Homo sapiens
144 PUS7 54517
Co-fractionation Homo sapiens
145 RRP7A 27341
Co-fractionation Homo sapiens
146 CDK2 1017
Affinity Capture-MS Homo sapiens
147 ZW10 9183
Co-fractionation Homo sapiens
148 SSR3 6747
Co-fractionation Homo sapiens
149 DKC1 1736
Co-fractionation Homo sapiens
150 MYC  
Affinity Capture-MS Homo sapiens
151 RPA3 6119
Proximity Label-MS Homo sapiens
152 LSM3 27258
Affinity Capture-MS Homo sapiens
153 RPS13 6207
Co-fractionation Homo sapiens
154 ZC3H18  
Co-fractionation Homo sapiens
155 DLST 1743
Affinity Capture-MS Homo sapiens
156 RPS14 6208
Co-fractionation Homo sapiens
157 TOP1 7150
Affinity Capture-MS Homo sapiens
158 NHP2L1 4809
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
159 SIRT6  
Affinity Capture-MS Homo sapiens
160 SART3 9733
Affinity Capture-MS Homo sapiens
161 CORO1A 11151
Co-fractionation Homo sapiens
162 DDB1 1642
Co-fractionation Homo sapiens
163 RPL23A 6147
Co-fractionation Homo sapiens
164 RPL18 6141
Co-fractionation Homo sapiens
165 RNF2  
Affinity Capture-MS Homo sapiens
166 BMI1  
Affinity Capture-MS Homo sapiens
167 TPR 7175
Co-fractionation Homo sapiens
168 KIAA0368 23392
Co-fractionation Homo sapiens
169 NOP16 51491
Co-fractionation Homo sapiens
170 PABPC3 5042
Co-fractionation Homo sapiens
171 RPS15A 6210
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
172 CORO1C 23603
Co-fractionation Homo sapiens
173 SUPT16H 11198
Co-fractionation Homo sapiens
174 GNL3 26354
Co-fractionation Homo sapiens
175 RPL19 6143
Co-fractionation Homo sapiens
176 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
177 HSPB2  
Two-hybrid Homo sapiens
178 CPSF6 11052
Affinity Capture-MS Homo sapiens
179 RAD17  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
180 RPL6 6128
Co-fractionation Homo sapiens
181 XRCC6 2547
Two-hybrid Homo sapiens
182 RPLP0P6 220717
Co-fractionation Homo sapiens
183 SF3B4 10262
Co-fractionation Homo sapiens
184 MEN1 4221
Affinity Capture-MS Homo sapiens
185 RPAP1  
Co-fractionation Homo sapiens
186 BOP1 23246
Co-fractionation Homo sapiens
187 SNRPA 6626
Affinity Capture-MS Homo sapiens
188 HNRNPM 4670
Co-fractionation Homo sapiens
189 SMURF1 57154
Affinity Capture-MS Homo sapiens
190 LSM5  
Affinity Capture-MS Homo sapiens
191 GTPBP1 9567
Co-fractionation Homo sapiens
192 RPS5 6193
Co-fractionation Homo sapiens
193 TARS 6897
Co-fractionation Homo sapiens
194 NIFK 84365
Co-fractionation Homo sapiens
195 HNRNPK 3190
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
196 WDR18 57418
Co-fractionation Homo sapiens
197 Prpf3 70767
Affinity Capture-MS Mus musculus
198 PCNA 5111
Co-fractionation Homo sapiens
199 TRIM55  
Co-fractionation Homo sapiens
200 LSM7  
Affinity Capture-MS Homo sapiens
201 RPS24 6229
Co-fractionation Homo sapiens
202 CDV3 55573
Co-fractionation Homo sapiens
203 HNRNPL 3191
Co-fractionation Homo sapiens
204 RPS23 6228
Co-fractionation Homo sapiens
205 SRSF7 6432
Co-fractionation Homo sapiens
206 NUFIP1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
207 RAB11A 8766
Affinity Capture-MS Homo sapiens
208 CIT 11113
Affinity Capture-MS Homo sapiens
209 SERBP1 26135
Affinity Capture-MS Homo sapiens
210 LSM6 11157
Affinity Capture-MS Homo sapiens
211 PRPF4 9128
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
212 SNW1 22938
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
213 HNRNPU 3192
Co-fractionation Homo sapiens
214 RC3H1 149041
Affinity Capture-MS Homo sapiens
215 PTRF 284119
Co-fractionation Homo sapiens
216 MATN2 4147
Affinity Capture-MS Homo sapiens
217 DDX56  
Co-fractionation Homo sapiens
218 RRS1 23212
Co-fractionation Homo sapiens
219 ZNHIT3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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