Gene description for MCM6
Gene name
minichromosome maintenance complex component 6
Gene symbol
MCM6
Other names/aliases
MCG40308 Mis5 P105MCM
Species
Homo sapiens
Database cross references - MCM6
ExoCarta
ExoCarta_4175
Entrez Gene
4175
HGNC
6949
MIM
601806
UniProt
Q14566
MCM6 identified in exosomes derived from the following tissue/cell type
Endothelial cells
26027894
Ovarian cancer cells
23333927
Ovarian cancer cells
23333927
Thymus
23844026
Gene ontology annotations for MCM6
Experiment description of studies that identified MCM6 in exosomes
1
Experiment ID
226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘
EV Particle analysis
Identified molecule
mRNA
Identification method
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID
26027894
Organism
Homo sapiens
Experiment description
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name
Journal of Extracellular Vesicles
Publication year
2015
Sample
Endothelial cells
Sample name
HMEC-1
Isolation/purification methods
Differential ultracentrifugation Sucrose density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA
Methods used in the study
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting
2
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for MCM6
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
SCNM1
Two-hybrid
Homo sapiens
2
PSMA1
5682
Two-hybrid
Homo sapiens
3
MCM10
55388
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
4
IL1F5
Two-hybrid
Homo sapiens
5
MCM7
4176
Invivo
Homo sapiens
Invivo
Homo sapiens
Invivo
Homo sapiens
Invivo
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
6
MCM3
4172
Affinity Capture-MS
Homo sapiens
7
RPA1
6117
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
8
MCM8
84515
Affinity Capture-Western
Homo sapiens
9
GMNN
Affinity Capture-MS
Homo sapiens
10
ORC1L
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
11
MLLT3
4300
Two-hybrid
Homo sapiens
12
CDC45L
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
13
MCM6
4175
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
14
MCM4
4173
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
15
ORC2L
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
16
CCNA1
Affinity Capture-MS
Homo sapiens
17
MCM2
4171
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
18
ALS2CR11
Two-hybrid
Homo sapiens
19
ING5
Two-hybrid
Homo sapiens
20
ORC4L
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
21
CDT1
Invitro
Homo sapiens
Invivo
Homo sapiens
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which MCM6 is involved