Gene description for PPP6R3
Gene name protein phosphatase 6, regulatory subunit 3
Gene symbol PPP6R3
Other names/aliases C11orf23
PP6R3
SAP190
SAPL
SAPLa
SAPS3
Species Homo sapiens
 Database cross references - PPP6R3
ExoCarta ExoCarta_55291
Vesiclepedia VP_55291
Entrez Gene 55291
HGNC 1173
MIM 610879
UniProt Q5H9R7  
 PPP6R3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PPP6R3
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase regulator activity GO:0019888 IBA
    protein phosphatase regulator activity GO:0019888 IDA
    protein phosphatase binding GO:0019903 IEA
Biological Process
    regulation of signal transduction GO:0009966 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified PPP6R3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPP6R3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 TNFAIP3 7128
Affinity Capture-MS Homo sapiens
3 HNRNPC 3183
Co-fractionation Homo sapiens
4 SENP3 26168
Affinity Capture-MS Homo sapiens
5 AP2B1 163
Proximity Label-MS Homo sapiens
6 HOOK1  
Proximity Label-MS Homo sapiens
7 DNAJC11 55735
Proximity Label-MS Homo sapiens
8 HDAC2 3066
Affinity Capture-MS Homo sapiens
9 CLIP1 6249
Affinity Capture-MS Homo sapiens
10 SP1  
Affinity Capture-MS Homo sapiens
11 EPB41L5 57669
Affinity Capture-MS Homo sapiens
12 AP2A1 160
Affinity Capture-MS Homo sapiens
13 BRCA1 672
Affinity Capture-MS Homo sapiens
14 RPL10 6134
Affinity Capture-MS Homo sapiens
15 C9orf78 51759
Affinity Capture-MS Homo sapiens
16 HSP90AB1 3326
Co-fractionation Homo sapiens
17 KDM6A  
Affinity Capture-MS Homo sapiens
18 TERF2IP 54386
Two-hybrid Homo sapiens
19 DIEXF  
Co-fractionation Homo sapiens
20 RPS6KB2  
Affinity Capture-MS Homo sapiens
21 ANKRD28 23243
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
22 PRKDC 5591
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
23 DNAJB6 10049
Affinity Capture-MS Homo sapiens
24 USP36  
Affinity Capture-MS Homo sapiens
25 PPP1R12A 4659
Co-fractionation Homo sapiens
26 TGFB1 7040
Affinity Capture-MS Homo sapiens
27 PPP6R2 9701
Proximity Label-MS Homo sapiens
28 VARS 7407
Co-fractionation Homo sapiens
29 GNS 2799
Co-fractionation Homo sapiens
30 IQGAP1 8826
Co-fractionation Homo sapiens
31 MRPL12 6182
Affinity Capture-MS Homo sapiens
32 PPP6R1 22870
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
33 SMC2 10592
Co-fractionation Homo sapiens
34 TINF2  
Two-hybrid Homo sapiens
35 HADHA 3030
Co-fractionation Homo sapiens
36 HSP90B1 7184
Co-fractionation Homo sapiens
37 FOXD4L6  
Affinity Capture-MS Homo sapiens
38 TULP3 7289
Affinity Capture-MS Homo sapiens
39 ICAM4  
Affinity Capture-MS Homo sapiens
40 ETFA 2108
Co-fractionation Homo sapiens
41 FOXD4  
Affinity Capture-MS Homo sapiens
42 USP7 7874
Co-fractionation Homo sapiens
43 MTA2 9219
Co-fractionation Homo sapiens
44 KIF14 9928
Affinity Capture-MS Homo sapiens
45 HSPA8 3312
Co-fractionation Homo sapiens
46 UNK  
Affinity Capture-RNA Homo sapiens
47 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
48 TOLLIP 54472
Proximity Label-MS Homo sapiens
49 GPSM1 26086
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 USP10 9100
Proximity Label-MS Homo sapiens
51 USP21  
Affinity Capture-MS Homo sapiens
52 PARK2  
Affinity Capture-MS Homo sapiens
53 MAP1B 4131
Co-fractionation Homo sapiens
54 Ppp6c 67857
Affinity Capture-MS Mus musculus
55 SIRT7  
Affinity Capture-MS Homo sapiens
56 SNAI2  
Affinity Capture-MS Homo sapiens
57 SRRT 51593
Co-fractionation Homo sapiens
58 C12orf49  
Negative Genetic Homo sapiens
59 Ankrd28  
Affinity Capture-MS Mus musculus
60 TNPO2 30000
Co-fractionation Homo sapiens
61 MYH9 4627
Co-fractionation Homo sapiens
62 GATA3  
Affinity Capture-MS Homo sapiens
63 ARRDC2  
Affinity Capture-MS Homo sapiens
64 NTRK1 4914
Affinity Capture-MS Homo sapiens
65 DNAJA1 3301
Co-fractionation Homo sapiens
66 Arhgef2  
Affinity Capture-MS Mus musculus
67 CSNK1E 1454
Affinity Capture-MS Homo sapiens
68 MYH10 4628
Co-fractionation Homo sapiens
69 MBD3 53615
Co-fractionation Homo sapiens
70 TRMT2A 27037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 ANKRD44 91526
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
72 H2AFY 9555
Co-fractionation Homo sapiens
73 ENAH 55740
Co-fractionation Homo sapiens
74 Ksr1  
Affinity Capture-MS Mus musculus
75 RAB40C 57799
Affinity Capture-Western Homo sapiens
76 EMID1  
Affinity Capture-MS Homo sapiens
77 C17orf53  
Proximity Label-MS Homo sapiens
78 ECH1 1891
Proximity Label-MS Homo sapiens
79 HSPA5 3309
Co-fractionation Homo sapiens
80 MOB1B 92597
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
81 CUL3 8452
Affinity Capture-MS Homo sapiens
82 AHCYL1 10768
Co-fractionation Homo sapiens
83 TIPRL 261726
Co-fractionation Homo sapiens
84 XPO1 7514
Affinity Capture-MS Homo sapiens
85 ARRDC4  
Affinity Capture-MS Homo sapiens
86 ECT2 1894
Affinity Capture-MS Homo sapiens
87 DNAJC2 27000
Proximity Label-MS Homo sapiens
88 ACAD11 84129
Affinity Capture-MS Homo sapiens
89 PRG2  
Affinity Capture-MS Homo sapiens
90 TFAP4 7023
Affinity Capture-MS Homo sapiens
91 HOOK3 84376
Proximity Label-MS Homo sapiens
92 AP1B1 162
Affinity Capture-MS Homo sapiens
93 TROVE2 6738
Co-fractionation Homo sapiens
94 MLF1  
Affinity Capture-MS Homo sapiens
95 ANKRD52 283373
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
96 IRF5  
Proximity Label-MS Homo sapiens
97 AP2A2 161
Affinity Capture-MS Homo sapiens
98 PPP6C 5537
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
99 DDRGK1 65992
Affinity Capture-MS Homo sapiens
100 RNF2  
Affinity Capture-MS Homo sapiens
101 WNT2  
Affinity Capture-MS Homo sapiens
102 HUWE1 10075
Affinity Capture-MS Homo sapiens
103 TPM3 7170
Co-fractionation Homo sapiens
104 PFAS 5198
Co-fractionation Homo sapiens
105 GRK5 2869
Affinity Capture-MS Homo sapiens
106 MOB1A 55233
Affinity Capture-Western Homo sapiens
107 FBXL6  
Affinity Capture-MS Homo sapiens
108 SIRT6  
Affinity Capture-MS Homo sapiens
109 USP49 25862
Affinity Capture-MS Homo sapiens
110 MCAT 27349
Affinity Capture-MS Homo sapiens
111 RUVBL2 10856
Co-fractionation Homo sapiens
112 WNT10B 7480
Affinity Capture-MS Homo sapiens
113 ZNF746  
Affinity Capture-MS Homo sapiens
114 CDC37 11140
Co-fractionation Homo sapiens
115 CTSG 1511
Affinity Capture-MS Homo sapiens
116 ERLIN2 11160
Proximity Label-MS Homo sapiens
117 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
118 HDAC1 3065
Affinity Capture-MS Homo sapiens
119 HSP90AA1 3320
Co-fractionation Homo sapiens
120 EIF4A3 9775
Co-fractionation Homo sapiens
121 GNL1 2794
Co-fractionation Homo sapiens
122 PSMA1 5682
Co-fractionation Homo sapiens
123 UFL1 23376
Affinity Capture-MS Homo sapiens
124 TPM1 7168
Co-fractionation Homo sapiens
125 ZNRF2 223082
Affinity Capture-Western Homo sapiens
126 KDM1A 23028
Co-fractionation Homo sapiens
127 C16orf72 29035
Affinity Capture-MS Homo sapiens
128 SERBP1 26135
Affinity Capture-MS Homo sapiens
129 C9orf72  
Affinity Capture-MS Homo sapiens
130 GPHA2  
Affinity Capture-MS Homo sapiens
131 SP7  
Affinity Capture-MS Homo sapiens
132 NOTCH1 4851
Affinity Capture-MS Homo sapiens
133 NSUN5  
Co-fractionation Homo sapiens
View the network image/svg+xml



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