Gene description for LPCAT1
Gene name lysophosphatidylcholine acyltransferase 1
Gene symbol LPCAT1
Other names/aliases AYTL2
PFAAP3
lpcat
Species Homo sapiens
 Database cross references - LPCAT1
ExoCarta ExoCarta_79888
Vesiclepedia VP_79888
Entrez Gene 79888
HGNC 25718
MIM 610472
UniProt Q8NF37  
 LPCAT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LPCAT1
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 ISS
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 TAS
    calcium ion binding GO:0005509 IEA
    lysophosphatidic acid acyltransferase activity GO:0042171 IBA
    2-acylglycerol-3-phosphate O-acyltransferase activity GO:0047144 TAS
    plasmalogen synthase activity GO:0047159 ISS
    1-acylglycerophosphocholine O-acyltransferase activity GO:0047184 IDA
    1-acylglycerophosphocholine O-acyltransferase activity GO:0047184 ISS
    1-alkylglycerophosphocholine O-acyltransferase activity GO:0047191 IEA
    1-alkylglycerophosphocholine O-acetyltransferase activity GO:0047192 ISS
Biological Process
    phosphatidic acid biosynthetic process GO:0006654 TAS
    phosphatidylcholine biosynthetic process GO:0006656 IEA
    phospholipid biosynthetic process GO:0008654 ISS
    protein catabolic process GO:0030163 IEA
    phosphatidylglycerol acyl-chain remodeling GO:0036148 TAS
    phosphatidylcholine acyl-chain remodeling GO:0036151 IDA
    phosphatidylcholine acyl-chain remodeling GO:0036151 TAS
    surfactant homeostasis GO:0043129 IEA
    positive regulation of protein catabolic process GO:0045732 IEA
    retina development in camera-type eye GO:0060041 IEA
    negative regulation of phosphatidylcholine biosynthetic process GO:2001246 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 ISS
    lipid droplet GO:0005811 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
    azurophil granule membrane GO:0035577 TAS
 Experiment description of studies that identified LPCAT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LPCAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIPK4  
Affinity Capture-MS Homo sapiens
2 REEP5 7905
Proximity Label-MS Homo sapiens
3 RAMP3  
Affinity Capture-MS Homo sapiens
4 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 BTRC 8945
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
7 FASN 2194
Negative Genetic Homo sapiens
8 SPRTN  
Affinity Capture-MS Homo sapiens
9 FAF2 23197
Co-fractionation Homo sapiens
10 DIP2A 23181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 MTR 4548
Affinity Capture-MS Homo sapiens
12 ILF3 3609
Co-fractionation Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 GSK3B 2932
Biochemical Activity Homo sapiens
15 FAS 355
Proximity Label-MS Homo sapiens
16 UBE2H 7328
Affinity Capture-MS Homo sapiens
17 ECT2 1894
Affinity Capture-MS Homo sapiens
18 MFSD4  
Affinity Capture-MS Homo sapiens
19 NF2 4771
Affinity Capture-MS Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 TPTE  
Affinity Capture-MS Homo sapiens
22 BCAP31 10134
Proximity Label-MS Homo sapiens
23 TSPAN15 23555
Affinity Capture-MS Homo sapiens
24 NR3C1 2908
Proximity Label-MS Homo sapiens
25 HSD17B11 51170
Proximity Label-MS Homo sapiens
26 CTDP1 9150
Affinity Capture-MS Homo sapiens
27 KLRD1  
Affinity Capture-MS Homo sapiens
28 SLC7A5 8140
Affinity Capture-MS Homo sapiens
29 PARK2  
Affinity Capture-MS Homo sapiens
30 CLPP 8192
Proximity Label-MS Homo sapiens
31 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
32 KIF23 9493
Affinity Capture-MS Homo sapiens
33 DOCK7 85440
Affinity Capture-MS Homo sapiens
34 DDX58 23586
Affinity Capture-RNA Homo sapiens
35 RIPK4  
Affinity Capture-MS Homo sapiens
36 MSTO1 55154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
38 ACBD4  
Affinity Capture-MS Homo sapiens
39 AUP1 550
Affinity Capture-MS Homo sapiens
40 KIAA1429 25962
Affinity Capture-MS Homo sapiens
41 RPA3 6119
Proximity Label-MS Homo sapiens
42 PARS2 25973
Affinity Capture-MS Homo sapiens
43 SEC62 7095
Proximity Label-MS Homo sapiens
44 ATP2A1 487
Proximity Label-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 PCK1 5105
Affinity Capture-MS Homo sapiens
47 GPR182  
Affinity Capture-MS Homo sapiens
48 AMFR 267
Affinity Capture-MS Homo sapiens
49 PEX6  
Affinity Capture-MS Homo sapiens
50 GLB1 2720
Affinity Capture-MS Homo sapiens
51 BRD1 23774
Affinity Capture-MS Homo sapiens
52 FBXW7  
Affinity Capture-MS Homo sapiens
53 SERBP1 26135
Affinity Capture-MS Homo sapiens
54 SAP25  
Affinity Capture-MS Homo sapiens
55 MARCH4  
Affinity Capture-MS Homo sapiens
56 RPN1 6184
Proximity Label-MS Homo sapiens
57 MDC1  
Affinity Capture-MS Homo sapiens
58 SLC27A2 11001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 ILK 3611
Affinity Capture-MS Homo sapiens
60 SSR1 6745
Proximity Label-MS Homo sapiens
61 MAP3K15  
Affinity Capture-MS Homo sapiens
62 BBS2 583
Affinity Capture-MS Homo sapiens
63 PEX3 8504
Proximity Label-MS Homo sapiens
64 CAV1 857
Proximity Label-MS Homo sapiens
65 LAMTOR3 8649
Co-fractionation Homo sapiens
66 RPN2 6185
Proximity Label-MS Homo sapiens
67 DERL1 79139
Proximity Label-MS Homo sapiens
68 OGT 8473
Reconstituted Complex Homo sapiens
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