Experiment description of studies that identified HIST1H4G in sEVs

1 Experiment ID 76 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI Enriched markers ✔ HSP90B1 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20224111     Organism Homo sapiens Experiment description Proteomics analysis of bladder cancer exosomes. Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A." Journal name MCP Publication year 2010 Sample Bladder cancer cells Sample name HT1376 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.10-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF/TOF] Western blotting FACS

2 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for HIST1H4G

Protein Interactor ExoCarta ID Identification method PubMed Species 1 H2AFZ 3015 Affinity Capture-Western Homo sapiens 2 TMED8 283578 Two-hybrid Homo sapiens 3 AGTRAP 57085 Two-hybrid Homo sapiens 4 APOC1   Two-hybrid Homo sapiens 5 HIST1H2BL 8340 Cross-Linking-MS (XL-MS) Homo sapiens 6 VRK1 7443 Affinity Capture-MS Homo sapiens 7 C2orf47   Two-hybrid Homo sapiens 8 MYCN   Affinity Capture-MS Homo sapiens 9 CIDEB   Two-hybrid Homo sapiens 10 HIST1H2BK 85236 Cross-Linking-MS (XL-MS) Homo sapiens 11 KIF20A 10112 Affinity Capture-MS Homo sapiens 12 CHMP4B 128866 Affinity Capture-MS Homo sapiens 13 PRC1 9055 Affinity Capture-MS Homo sapiens 14 HIST1H2BD 3017 Cross-Linking-MS (XL-MS) Homo sapiens 15 HMGN1   Affinity Capture-MS Homo sapiens 16 KIF23 9493 Affinity Capture-MS Homo sapiens 17 FGF14   Two-hybrid Homo sapiens 18 ANLN 54443 Affinity Capture-MS Homo sapiens 19 HIST1H4A 8359 Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 20 HIST2H2BE 8349 Cross-Linking-MS (XL-MS) Homo sapiens 21 ECT2 1894 Affinity Capture-MS Homo sapiens View the network

Pathways in which HIST1H4G is involved

No pathways found