Experiment description of studies that identified GSTK1 in exosomes

1 Experiment ID 207 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 ISEV standards ✘ EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 66 ISEV standards ✔ IEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ CD63|CD81 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19199708     Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

5 Experiment ID 191 ISEV standards ✘ EV Biophysical techniques ✔ Alix EV Cytosolic markers ✔ CD81|CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20124223     Organism Homo sapiens Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. Journal name Mol Cell Proteomics Publication year 2010 Sample Squamous carcinoma cells Sample name Squamous carcinoma cell (A431) Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for GSTK1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 MCM7 4176 Affinity Capture-MS Homo sapiens 2 RPN2 6185 Affinity Capture-MS Homo sapiens 3 HNRNPM 4670 Affinity Capture-MS Homo sapiens 4 PDCD6IP 10015 Affinity Capture-MS Homo sapiens 5 HRNR 388697 Affinity Capture-MS Homo sapiens 6 EPPK1 83481 Affinity Capture-MS Homo sapiens 7 DNAAF5 54919 Affinity Capture-MS Homo sapiens 8 FANCI 55215 Affinity Capture-MS Homo sapiens 9 ATXN10 25814 Affinity Capture-MS Homo sapiens 10 CCAR2 57805 Affinity Capture-MS Homo sapiens 11 CYCS   Affinity Capture-MS Homo sapiens 12 XPO5 57510 Affinity Capture-MS Homo sapiens 13 LRPPRC 10128 Affinity Capture-MS Homo sapiens 14 EFTUD2 9343 Affinity Capture-MS Homo sapiens 15 ZW10 9183 Affinity Capture-MS Homo sapiens 16 KIF1BP 26128 Affinity Capture-MS Homo sapiens 17 SLC3A2 6520 Affinity Capture-MS Homo sapiens 18 IPO4 79711 Affinity Capture-MS Homo sapiens 19 TSC22D1   Affinity Capture-MS Homo sapiens 20 DCTN1 1639 Affinity Capture-MS Homo sapiens 21 MATR3 9782 Affinity Capture-MS Homo sapiens 22 RPN1 6184 Affinity Capture-MS Homo sapiens 23 HTR1B   Affinity Capture-MS Homo sapiens 24 IARS 3376 Affinity Capture-MS Homo sapiens 25 DDOST 1650 Affinity Capture-MS Homo sapiens 26 SEC63   Affinity Capture-MS Homo sapiens View the network

Pathways in which GSTK1 is involved

No pathways found