Gene ontology annotations for TRA2B
Experiment description of studies that identified TRA2B in sEVs
1
Experiment ID
489
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
412
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MCF7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
6
Experiment ID
414
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
7
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
10
Experiment ID
498
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
11
Experiment ID
407
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
12
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
13
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
14
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
15
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
16
Experiment ID
405
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Foreskin fibroblasts
Sample name
BJ
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
17
Experiment ID
234
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-C3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
18
Experiment ID
235
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
19
Experiment ID
236
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
20
Experiment ID
237
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
21
Experiment ID
417
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Lymphoma cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
22
Experiment ID
411
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Mammary cancer-associated fibroblasts
Sample name
mCAF
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
23
Experiment ID
488
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
24
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
25
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
26
Experiment ID
413
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Normal mammary epithelial cells
Sample name
MCF10A
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
27
Experiment ID
211
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
28
Experiment ID
212
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
29
Experiment ID
406
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
BxPC3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
30
Experiment ID
415
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
31
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
32
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
33
Experiment ID
408
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPDE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
34
Experiment ID
409
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPNE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
35
Experiment ID
416
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pluripotent stem cells
Sample name
PSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
36
Experiment ID
410
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
T lymphocytes
Sample name
Jurkat
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
Protein-protein interactions for TRA2B
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
HNRNPH1
3187
Affinity Capture-RNA
Homo sapiens
Affinity Capture-MS
Homo sapiens
2
UBE2H
7328
Affinity Capture-MS
Homo sapiens
3
HNRNPC
3183
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
4
FOXA1
Affinity Capture-MS
Homo sapiens
5
ZNF514
Affinity Capture-MS
Homo sapiens
6
RBMY1F
Two-hybrid
Homo sapiens
7
PAXIP1
Affinity Capture-MS
Homo sapiens
8
PPIE
10450
Affinity Capture-MS
Homo sapiens
9
SRPK2
6733
Biochemical Activity
Homo sapiens
Two-hybrid
Homo sapiens
10
YWHAE
7531
Affinity Capture-MS
Homo sapiens
11
Ybx1
22608
Affinity Capture-MS
Mus musculus
12
SOX2
Affinity Capture-MS
Homo sapiens
13
NGRN
Proximity Label-MS
Homo sapiens
14
KIF20A
10112
Affinity Capture-MS
Homo sapiens
15
MRPL27
51264
Affinity Capture-MS
Homo sapiens
16
MRPL50
54534
Affinity Capture-MS
Homo sapiens
17
VHL
Negative Genetic
Homo sapiens
18
CAND1
55832
Affinity Capture-MS
Homo sapiens
19
SRSF2
6427
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
20
SNRPF
6636
Affinity Capture-MS
Homo sapiens
21
SEPT2
4735
Co-fractionation
Homo sapiens
22
PRKDC
5591
Co-fractionation
Homo sapiens
23
GFM2
84340
Proximity Label-MS
Homo sapiens
24
CMBL
134147
Affinity Capture-MS
Homo sapiens
25
ILF2
3608
Co-fractionation
Homo sapiens
26
APEX1
328
Affinity Capture-RNA
Homo sapiens
27
PSIP1
11168
Co-fractionation
Homo sapiens
28
SNRNP70
6625
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
29
RNPS1
10921
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
30
KIF23
9493
Affinity Capture-MS
Homo sapiens
31
EPM2AIP1
9852
Two-hybrid
Homo sapiens
32
SF3B1
23451
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
33
CDK6
1021
Biochemical Activity
Homo sapiens
34
RBM14
10432
Affinity Capture-MS
Homo sapiens
35
COPS5
10987
Affinity Capture-MS
Homo sapiens
36
PRC1
9055
Affinity Capture-MS
Homo sapiens
37
MAGOHB
55110
Two-hybrid
Homo sapiens
38
SNRPC
6631
Affinity Capture-MS
Homo sapiens
39
POU5F1
Affinity Capture-RNA
Homo sapiens
40
YWHAG
7532
Affinity Capture-MS
Homo sapiens
41
MRPL21
219927
Affinity Capture-MS
Homo sapiens
42
OBSL1
23363
Affinity Capture-MS
Homo sapiens
43
SF3A2
8175
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
44
TRA2A
29896
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
45
HINFP
Two-hybrid
Homo sapiens
46
EED
Affinity Capture-MS
Homo sapiens
47
MRPL39
54148
Affinity Capture-MS
Homo sapiens
48
U2AF1
7307
Two-hybrid
Homo sapiens
49
KDM4A
Affinity Capture-MS
Homo sapiens
50
NHP2
55651
Affinity Capture-MS
Homo sapiens
51
MECP2
4204
Affinity Capture-MS
Homo sapiens
52
CUL1
8454
Affinity Capture-MS
Homo sapiens
53
ARIH2
10425
Affinity Capture-MS
Homo sapiens
54
MRPL4
51073
Affinity Capture-MS
Homo sapiens
55
FAF2
23197
Co-fractionation
Homo sapiens
56
SREK1
140890
Affinity Capture-MS
Homo sapiens
57
MARCH5
Proximity Label-MS
Homo sapiens
58
MTERF3
Proximity Label-MS
Homo sapiens
59
KIF14
9928
Affinity Capture-MS
Homo sapiens
60
PRNP
5621
Affinity Capture-MS
Homo sapiens
61
TRIM31
Affinity Capture-MS
Homo sapiens
62
RBMX
27316
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
63
UNK
Affinity Capture-RNA
Homo sapiens
64
CHMP4C
92421
Affinity Capture-MS
Homo sapiens
65
CLK2
1196
Affinity Capture-MS
Homo sapiens
66
C6orf203
Proximity Label-MS
Homo sapiens
67
HIST1H4A
8359
Affinity Capture-MS
Homo sapiens
68
DDX21
9188
Affinity Capture-MS
Homo sapiens
69
RB1CC1
9821
Affinity Capture-MS
Homo sapiens
70
PARK2
Affinity Capture-MS
Homo sapiens
71
Ddb1
13194
Affinity Capture-MS
Mus musculus
72
SRSF3
6428
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
73
BRD3
8019
Affinity Capture-MS
Homo sapiens
74
SRSF6
6431
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
75
SNRNP200
23020
Co-fractionation
Homo sapiens
76
RPL5
6125
Affinity Capture-MS
Homo sapiens
77
SNRPB
6628
Affinity Capture-MS
Homo sapiens
78
SNIP1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
79
NFIA
4774
Co-fractionation
Homo sapiens
80
BTF3
689
Affinity Capture-MS
Homo sapiens
81
RAN
5901
Co-fractionation
Homo sapiens
82
NTRK1
4914
Affinity Capture-MS
Homo sapiens
83
TNPO3
23534
Affinity Capture-MS
Homo sapiens
84
U2AF2
11338
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
85
RAD18
Affinity Capture-MS
Homo sapiens
86
DNAJA1
3301
Co-fractionation
Homo sapiens
87
LUC7L2
51631
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
88
BANF1
8815
Affinity Capture-MS
Homo sapiens
89
TRUB2
Proximity Label-MS
Homo sapiens
90
MAGOH
4116
Affinity Capture-MS
Homo sapiens
91
CUL2
8453
Affinity Capture-MS
Homo sapiens
92
BRD4
23476
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
93
DDX39B
7919
Affinity Capture-MS
Homo sapiens
94
NUDC
10726
Cross-Linking-MS (XL-MS)
Homo sapiens
95
CEBPA
Affinity Capture-MS
Homo sapiens
96
CYLD
Affinity Capture-MS
Homo sapiens
97
SRPK1
6732
Biochemical Activity
Homo sapiens
98
BARD1
580
Affinity Capture-MS
Homo sapiens
99
ELAVL2
1993
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
100
SRSF11
9295
Affinity Capture-MS
Homo sapiens
101
SNRPD2
6633
Affinity Capture-MS
Homo sapiens
102
ZBTB2
57621
Affinity Capture-MS
Homo sapiens
103
RBM39
9584
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
104
PRPF4B
8899
Affinity Capture-MS
Homo sapiens
105
PPP4R2
Co-fractionation
Homo sapiens
106
C11orf57
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
107
TOP1
7150
Affinity Capture-MS
Homo sapiens
108
SRSF7
6432
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
109
CLK3
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
110
CHCHD1
Proximity Label-MS
Homo sapiens
111
RC3H2
Affinity Capture-MS
Homo sapiens
112
ECT2
1894
Affinity Capture-MS
Homo sapiens
113
MTX2
10651
Proximity Label-MS
Homo sapiens
114
SRSF4
6429
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
115
SRSF1
6426
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
116
RBMY1J
Two-hybrid
Homo sapiens
117
CUL3
8452
Affinity Capture-MS
Homo sapiens
118
YWHAZ
7534
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
119
SRSF5
6430
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
120
EGFR
1956
Affinity Capture-MS
Homo sapiens
121
HNRNPA1
3178
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
122
FUS
2521
Affinity Capture-MS
Homo sapiens
123
BAP1
8314
Affinity Capture-MS
Homo sapiens
124
MYC
Affinity Capture-MS
Homo sapiens
125
NXF1
10482
Two-hybrid
Homo sapiens
126
SF3A1
10291
Affinity Capture-MS
Homo sapiens
127
YTHDC1
Two-hybrid
Homo sapiens
128
C12orf65
Proximity Label-MS
Homo sapiens
129
SETD1A
9739
Affinity Capture-MS
Homo sapiens
130
EIF4H
7458
Co-fractionation
Homo sapiens
131
MRPL19
9801
Affinity Capture-MS
Homo sapiens
132
TADA2A
Affinity Capture-MS
Homo sapiens
133
MYO6
4646
Proximity Label-MS
Homo sapiens
134
PSPC1
55269
Affinity Capture-MS
Homo sapiens
135
BAG1
573
Affinity Capture-MS
Homo sapiens
136
SLC3A2
6520
Co-fractionation
Homo sapiens
137
ZC3H18
Affinity Capture-MS
Homo sapiens
138
PABPC5
140886
Affinity Capture-MS
Homo sapiens
139
MRPL49
740
Affinity Capture-MS
Homo sapiens
140
NHP2L1
4809
Co-fractionation
Homo sapiens
141
MYCN
Affinity Capture-MS
Homo sapiens
142
SRSF10
10772
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
143
DDRGK1
65992
Affinity Capture-MS
Homo sapiens
144
SEPT7
989
Co-fractionation
Homo sapiens
145
MRPL38
Affinity Capture-MS
Homo sapiens
146
HIST1H1T
3010
Affinity Capture-MS
Homo sapiens
147
GCN1L1
10985
Co-fractionation
Homo sapiens
148
RBMS2
5939
Co-fractionation
Homo sapiens
149
CCDC8
Affinity Capture-MS
Homo sapiens
150
RPS16
6217
Affinity Capture-MS
Homo sapiens
151
PROSC
11212
Affinity Capture-MS
Homo sapiens
152
YBX1
4904
Affinity Capture-MS
Homo sapiens
153
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
154
SRSF8
10929
Two-hybrid
Homo sapiens
155
PARP1
142
Proximity Label-MS
Homo sapiens
156
ILF3
3609
Co-fractionation
Homo sapiens
157
ZRANB2
9406
Protein-RNA
Homo sapiens
158
CPSF6
11052
Affinity Capture-MS
Homo sapiens
159
TP53
7157
Affinity Capture-MS
Homo sapiens
160
EIF3I
8668
Co-fractionation
Homo sapiens
161
SRSF9
8683
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
162
SAP18
10284
Affinity Capture-MS
Homo sapiens
163
CUL5
8065
Affinity Capture-MS
Homo sapiens
164
SNRPA
6626
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
165
LOC102724594
102724594
Two-hybrid
Homo sapiens
166
NHLRC2
374354
Affinity Capture-MS
Homo sapiens
167
EIF4A3
9775
Affinity Capture-MS
Homo sapiens
168
TRA2B
6434
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
169
S100A9
6280
Co-fractionation
Homo sapiens
170
MRPL18
29074
Affinity Capture-MS
Homo sapiens
171
UFL1
23376
Affinity Capture-MS
Homo sapiens
172
PCDHB14
Two-hybrid
Homo sapiens
173
HNRNPL
3191
Affinity Capture-MS
Homo sapiens
174
GSPT2
23708
Affinity Capture-MS
Homo sapiens
175
FLNA
2316
Co-fractionation
Homo sapiens
176
MYPOP
Two-hybrid
Homo sapiens
177
GRSF1
2926
Proximity Label-MS
Homo sapiens
178
HNRNPU
3192
Co-fractionation
Homo sapiens
179
C9orf72
Affinity Capture-MS
Homo sapiens
180
RC3H1
149041
Affinity Capture-MS
Homo sapiens
181
Safb
Two-hybrid
Rattus norvegicus
Reconstituted Complex
Rattus norvegicus
182
HNRNPA2B1
3181
Co-fractionation
Homo sapiens
183
SSRP1
6749
Affinity Capture-MS
Homo sapiens
184
DDX47
51202
Co-fractionation
Homo sapiens
185
EP300
2033
Affinity Capture-MS
Homo sapiens
186
HIPK3
10114
Protein-RNA
Homo sapiens
View the network
image/svg+xml
Pathways in which TRA2B is involved