Gene description for GNAO1
Gene name guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Gene symbol GNAO1
Other names/aliases EIEE17
G-ALPHA-o
GNAO
Species Homo sapiens
 Database cross references - GNAO1
ExoCarta ExoCarta_2775
Entrez Gene 2775
HGNC 4389
MIM 139311
UniProt P09471  
 GNAO1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for GNAO1
Molecular Function
    GTPase activity GO:0003924 IEA
    GTPase activating protein binding GO:0032794 IEA
    corticotropin-releasing hormone receptor 1 binding GO:0051430 IBA
    signal transducer activity GO:0004871 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    mu-type opioid receptor binding GO:0031852 IBA
    metal ion binding GO:0046872 IEA
    GTP binding GO:0005525 IEA
    G-protein coupled serotonin receptor binding GO:0031821 IBA
Biological Process
    positive regulation of GTPase activity GO:0043547 IEA
    response to morphine GO:0043278 IEA
    muscle contraction GO:0006936 TAS
    forebrain development GO:0030900 IEA
    response to drug GO:0042493 IEA
    response to cytokine GO:0034097 IEA
    neuron projection development GO:0031175 IEA
    aging GO:0007568 IEA
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    dopamine receptor signaling pathway GO:0007212 IBA
    locomotory behavior GO:0007626 IEA
    metabolic process GO:0008152 TAS
    regulation of heart contraction GO:0008016 IEA
    negative regulation of calcium ion transport GO:0051926 IEA
    response to hydrogen peroxide GO:0042542 IEA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    neuron projection GO:0043005 IEA
    heterotrimeric G-protein complex GO:0005834 IBA
 Experiment description of studies that identified GNAO1 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 193
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
PHB
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 194
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 195
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNAO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRA2C  
Invivo Homo sapiens
2 RGS7  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 OPRM1 4988
Invivo Homo sapiens
4 RGS14  
Affinity Capture-MS Homo sapiens
5 SCN8A  
Invivo Homo sapiens
Invitro Homo sapiens
6 CACNA1B 774
Reconstituted Complex Homo sapiens
7 ADORA1  
Affinity Capture-MS Homo sapiens
8 EDG8  
Affinity Capture-MS Homo sapiens
9 RGS19  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 OPRD1  
Invivo Homo sapiens
11 ADRA2A  
Invivo Homo sapiens
Invitro Homo sapiens
12 NGB  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
13 DCTN2 10540
Two-hybrid Homo sapiens
14 RIC8A 60626
Invitro Homo sapiens
Two-hybrid Homo sapiens
15 CRHR1  
Affinity Capture-Western Homo sapiens
16 RGS5  
Reconstituted Complex Homo sapiens
17 RGS16 6004
Reconstituted Complex Homo sapiens
18 RGS4  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GNAO1 is involved
PathwayEvidenceSource
Ca2+ pathway TAS Reactome
G-protein activation TAS Reactome
PLC beta mediated events TAS Reactome





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