Gene description for DNAJC10
Gene name DnaJ (Hsp40) homolog, subfamily C, member 10
Gene symbol DNAJC10
Other names/aliases ERdj5
JPDI
MTHr
PDIA19
Species Homo sapiens
 Database cross references - DNAJC10
ExoCarta ExoCarta_54431
Vesiclepedia VP_54431
Entrez Gene 54431
HGNC 24637
MIM 607987
UniProt Q8IXB1  
 DNAJC10 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for DNAJC10
Molecular Function
    ATPase activator activity GO:0001671 ISS
    protein binding GO:0005515 IPI
    protein-disulfide reductase activity GO:0015035 IBA
    protein-disulfide reductase activity GO:0015035 IDA
    protein-disulfide reductase activity GO:0015035 ISS
    disulfide oxidoreductase activity GO:0015036 ISS
    oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor GO:0016671 IBA
    oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor GO:0016671 ISS
    Hsp70 protein binding GO:0030544 IPI
    protein-folding chaperone binding GO:0051087 IDA
    ATPase binding GO:0051117 IPI
    misfolded protein binding GO:0051787 IBA
    misfolded protein binding GO:0051787 IDA
Biological Process
    negative regulation of protein phosphorylation GO:0001933 IDA
    positive regulation of ATP-dependent activity GO:0032781 ISS
    protein folding in endoplasmic reticulum GO:0034975 IDA
    response to endoplasmic reticulum stress GO:0034976 IDA
    IRE1-mediated unfolded protein response GO:0036498 IBA
    ERAD pathway GO:0036503 IMP
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 IBA
    endoplasmic reticulum lumen GO:0005788 IDA
    membrane GO:0016020 HDA
    endoplasmic reticulum chaperone complex GO:0034663 IDA
 Experiment description of studies that identified DNAJC10 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DNAJC10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 UGGT2 55757
Proximity Label-MS Homo sapiens
3 P4HA1 5033
Proximity Label-MS Homo sapiens
4 NOTCH3 4854
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SEP15 9403
Proximity Label-MS Homo sapiens
6 RPL38 6169
Cross-Linking-MS (XL-MS) Homo sapiens
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 RIN3  
Affinity Capture-MS Homo sapiens
9 MTX1 4580
Co-fractionation Homo sapiens
10 TRIM66  
Affinity Capture-MS Homo sapiens
11 ERAP2 64167
Proximity Label-MS Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 NENF 29937
Proximity Label-MS Homo sapiens
14 CAND1 55832
Affinity Capture-MS Homo sapiens
15 SCAF4 57466
Cross-Linking-MS (XL-MS) Homo sapiens
16 PRPF40A 55660
Cross-Linking-MS (XL-MS) Homo sapiens
17 PLOD1 5351
Proximity Label-MS Homo sapiens
18 TOMM22 56993
Co-fractionation Homo sapiens
19 CUL4B 8450
Affinity Capture-MS Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 EFNA3  
Affinity Capture-MS Homo sapiens
22 CAPZB 832
Affinity Capture-MS Homo sapiens
23 PLOD3 8985
Proximity Label-MS Homo sapiens
24 WDR76  
Affinity Capture-MS Homo sapiens
25 KIF23 9493
Affinity Capture-MS Homo sapiens
26 MCM2 4171
Affinity Capture-MS Homo sapiens
27 RCN2 5955
Proximity Label-MS Homo sapiens
28 COPS5 10987
Affinity Capture-MS Homo sapiens
29 NPM1 4869
Proximity Label-MS Homo sapiens
30 BRPF3  
Affinity Capture-MS Homo sapiens
31 DDOST 1650
Proximity Label-MS Homo sapiens
32 Cenpe  
Affinity Capture-MS Mus musculus
33 PDIA6 10130
Proximity Label-MS Homo sapiens
34 P3H1 64175
Proximity Label-MS Homo sapiens
35 TOMM40 10452
Co-fractionation Homo sapiens
36 PRDX4 10549
Proximity Label-MS Homo sapiens
37 SSFA2 6744
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ATP5H 10476
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
39 CALR 811
Proximity Label-MS Homo sapiens
40 MECP2 4204
Affinity Capture-MS Homo sapiens
41 CUL1 8454
Affinity Capture-MS Homo sapiens
42 MYCN  
Affinity Capture-MS Homo sapiens
43 NEDD8 4738
Affinity Capture-MS Homo sapiens
44 FAM20C 56975
Affinity Capture-MS Homo sapiens
45 KIF14 9928
Affinity Capture-MS Homo sapiens
46 EMC1 23065
Proximity Label-MS Homo sapiens
47 LMAN1 3998
Proximity Label-MS Homo sapiens
48 LRRC59 55379
Proximity Label-MS Homo sapiens
49 PPIB 5479
Proximity Label-MS Homo sapiens
50 NOMO1 23420
Proximity Label-MS Homo sapiens
51 FOXA1  
Affinity Capture-MS Homo sapiens
52 ERO1L 30001
Proximity Label-MS Homo sapiens
53 IKBIP 121457
Proximity Label-MS Homo sapiens
54 USP11 8237
Affinity Capture-MS Homo sapiens
55 RB1CC1 9821
Affinity Capture-MS Homo sapiens
56 PARK2  
Affinity Capture-MS Homo sapiens
57 PGAM5 192111
Co-fractionation Homo sapiens
58 HSP90B1 7184
Proximity Label-MS Homo sapiens
59 DNAJC5 80331
Cross-Linking-MS (XL-MS) Homo sapiens
60 TMTC3 160418
Proximity Label-MS Homo sapiens
61 MAGT1 84061
Proximity Label-MS Homo sapiens
62 ESRRB  
Affinity Capture-MS Homo sapiens
63 CHMP4B 128866
Affinity Capture-MS Homo sapiens
64 GRAMD4 23151
Affinity Capture-MS Homo sapiens
65 VCP 7415
Affinity Capture-Western Homo sapiens
66 NTRK1 4914
Affinity Capture-MS Homo sapiens
67 PI4KB 5298
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 POLDIP2 26073
Affinity Capture-MS Homo sapiens
69 ARRDC3 57561
Affinity Capture-MS Homo sapiens
70 RPN1 6184
Proximity Label-MS Homo sapiens
71 PCSK9 255738
Affinity Capture-MS Homo sapiens
72 FBLN5 10516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 HECTD1 25831
Affinity Capture-MS Homo sapiens
74 CUL2 8453
Affinity Capture-MS Homo sapiens
75 DNAJC30  
Proximity Label-MS Homo sapiens
76 LRP1B 53353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 MTMR4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 VDAC2 7417
Co-fractionation Homo sapiens
79 Ksr1  
Affinity Capture-MS Mus musculus
80 SEC61B 10952
Proximity Label-MS Homo sapiens
81 DNAJB11 51726
Proximity Label-MS Homo sapiens
82 NUP210 23225
Proximity Label-MS Homo sapiens
83 COX4I1 1327
Proximity Label-MS Homo sapiens
84 KDF1  
Affinity Capture-MS Homo sapiens
85 ANLN 54443
Affinity Capture-MS Homo sapiens
86 NUCB2 4925
Proximity Label-MS Homo sapiens
87 GAN 8139
Affinity Capture-MS Homo sapiens
88 HSPA5 3309
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
89 HADHB 3032
Co-fractionation Homo sapiens
90 CANX 821
Proximity Label-MS Homo sapiens
91 CALR3  
Proximity Label-MS Homo sapiens
92 MAGEA11  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
93 ECT2 1894
Affinity Capture-MS Homo sapiens
94 CLPP 8192
Proximity Label-MS Homo sapiens
95 AKAP9 10142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 RLIM 51132
Affinity Capture-MS Homo sapiens
97 BCAP31 10134
Proximity Label-MS Homo sapiens
98 PDIA3 2923
Proximity Label-MS Homo sapiens
99 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 LTBP3 4054
Affinity Capture-MS Homo sapiens
101 LTBP4 8425
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
103 FUS 2521
Affinity Capture-MS Homo sapiens
104 ERP29 10961
Proximity Label-MS Homo sapiens
105 RCN1 5954
Proximity Label-MS Homo sapiens
106 WAPAL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 MIA3 375056
Proximity Label-MS Homo sapiens
108 CDC42 998
Affinity Capture-MS Homo sapiens
109 GRAMD1A  
Proximity Label-MS Homo sapiens
110 AURKB 9212
Affinity Capture-MS Homo sapiens
111 UGGT1 56886
Proximity Label-MS Homo sapiens
112 ARIH2 10425
Affinity Capture-MS Homo sapiens
113 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
114 FBXO6 26270
Affinity Capture-MS Homo sapiens
115 MYC  
Affinity Capture-MS Homo sapiens
116 RPA3 6119
Proximity Label-MS Homo sapiens
117 NUCB1 4924
Proximity Label-MS Homo sapiens
118 Mis12  
Affinity Capture-MS Mus musculus
119 P4HB 5034
Proximity Label-MS Homo sapiens
120 TXNL1 9352
Co-fractionation Homo sapiens
121 N4BP2L2 10443
Affinity Capture-MS Homo sapiens
122 PPARGC1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 TNS2 23371
Affinity Capture-MS Homo sapiens
124 HSPA4 3308
Co-fractionation Homo sapiens
125 DNAJC3 5611
Proximity Label-MS Homo sapiens
126 HYOU1 10525
Proximity Label-MS Homo sapiens
127 TXNDC5 81567
Proximity Label-MS Homo sapiens
128 GANAB 23193
Proximity Label-MS Homo sapiens
129 LMNA 4000
Proximity Label-MS Homo sapiens
130 AKAP1 8165
Proximity Label-MS Homo sapiens
131 TTC13  
Proximity Label-MS Homo sapiens
132 CDC25A  
Affinity Capture-MS Homo sapiens
133 LAMB1 3912
Proximity Label-MS Homo sapiens
134 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
135 PRKCSH 5589
Proximity Label-MS Homo sapiens
136 GRN 2896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 SIRT6  
Affinity Capture-MS Homo sapiens
138 FOXRED2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
139 TRIM33 51592
Affinity Capture-MS Homo sapiens
140 MTMR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 PDIA4 9601
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
142 CALU 813
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
143 CUL5 8065
Affinity Capture-MS Homo sapiens
144 EDEM1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
145 SMURF1 57154
Affinity Capture-MS Homo sapiens
146 PTPN2 5771
Affinity Capture-MS Homo sapiens
147 LAMC1 3915
Proximity Label-MS Homo sapiens
148 TRAPPC13 80006
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 TARS 6897
Cross-Linking-MS (XL-MS) Homo sapiens
150 SFTPC  
Affinity Capture-Western Homo sapiens
151 PLD3 23646
Proximity Label-MS Homo sapiens
152 GNB1L 54584
Proximity Label-MS Homo sapiens
153 LRPAP1 4043
Proximity Label-MS Homo sapiens
154 LAMB2 3913
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
155 TCF3  
Affinity Capture-MS Homo sapiens
156 BRD1 23774
Affinity Capture-MS Homo sapiens
157 LDLR 3949
Affinity Capture-MS Homo sapiens
158 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
159 STT3B 201595
Proximity Label-MS Homo sapiens
160 HSPA4L 22824
Co-fractionation Homo sapiens
161 COLGALT1 79709
Proximity Label-MS Homo sapiens
162 C9orf72  
Affinity Capture-MS Homo sapiens
163 SMURF2 64750
Affinity Capture-MS Homo sapiens
164 VDAC3 7419
Co-fractionation Homo sapiens
165 CKAP4 10970
Proximity Label-MS Homo sapiens
166 TOMM5  
Co-fractionation Homo sapiens
167 CUL4A 8451
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNAJC10 is involved
No pathways found





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