Gene description for EMG1
Gene name EMG1 N1-specific pseudouridine methyltransferase
Gene symbol EMG1
Other names/aliases C2F
Grcc2f
NEP1
Species Homo sapiens
 Database cross references - EMG1
ExoCarta ExoCarta_10436
Vesiclepedia VP_10436
Entrez Gene 10436
HGNC 16912
MIM 611531
UniProt Q92979  
 EMG1 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for EMG1
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 ISS
    protein binding GO:0005515 IPI
    rRNA binding GO:0019843 IBA
    identical protein binding GO:0042802 IPI
    rRNA (pseudouridine) methyltransferase activity GO:0070037 IBA
    rRNA (pseudouridine) methyltransferase activity GO:0070037 IDA
Biological Process
    blastocyst development GO:0001824 IEA
    rRNA processing GO:0006364 ISS
    nucleologenesis GO:0017126 IEA
    ribosomal small subunit biogenesis GO:0042274 IDA
    ribosomal small subunit biogenesis GO:0042274 ISS
    rRNA base methylation GO:0070475 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    chromosome GO:0005694 IDA
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISS
    cytoplasm GO:0005737 ISS
    small-subunit processome GO:0032040 IBA
    small-subunit processome GO:0032040 IDA
 Experiment description of studies that identified EMG1 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EMG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 IL7R  
Protein-RNA Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 WIF1 11197
Affinity Capture-MS Homo sapiens
6 Eif3a 13669
Affinity Capture-MS Mus musculus
7 TIMM21  
Affinity Capture-MS Homo sapiens
8 LINGO2  
Affinity Capture-MS Homo sapiens
9 ICE2  
Affinity Capture-MS Homo sapiens
10 Klc4  
Two-hybrid Mus musculus
11 ZNF768 79724
Two-hybrid Homo sapiens
12 FAM98A 25940
Two-hybrid Homo sapiens
13 KIF20A 10112
Affinity Capture-MS Homo sapiens
14 ECT2 1894
Affinity Capture-MS Homo sapiens
15 NOP14 8602
Affinity Capture-MS Homo sapiens
16 ARHGAP26 23092
Affinity Capture-MS Homo sapiens
17 RPA2 6118
Affinity Capture-MS Homo sapiens
18 USP36  
Affinity Capture-MS Homo sapiens
19 RPA1 6117
Affinity Capture-MS Homo sapiens
20 KHDRBS1 10657
Two-hybrid Homo sapiens
21 RPL10 6134
Affinity Capture-MS Homo sapiens
22 BYSL 705
Co-fractionation Homo sapiens
23 ZCCHC9  
Two-hybrid Homo sapiens
24 CYP1A1  
Affinity Capture-MS Homo sapiens
25 NOC4L 79050
Affinity Capture-MS Homo sapiens
26 IPO5 3843
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 MILR1  
Affinity Capture-MS Homo sapiens
28 DDX58 23586
Affinity Capture-RNA Homo sapiens
29 SOX2  
Affinity Capture-MS Homo sapiens
30 APP 351
Reconstituted Complex Homo sapiens
31 FN1 2335
Affinity Capture-MS Homo sapiens
32 SMURF1 57154
Affinity Capture-MS Homo sapiens
33 GOLGA7 51125
Affinity Capture-MS Homo sapiens
34 EGFR 1956
Negative Genetic Homo sapiens
35 E2F4  
Affinity Capture-MS Homo sapiens
36 TCF4  
Affinity Capture-MS Homo sapiens
37 PARP1 142
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
38 ZNF106  
Affinity Capture-MS Homo sapiens
39 HECW2  
Affinity Capture-MS Homo sapiens
40 IPO4 79711
Affinity Capture-MS Homo sapiens
41 EMC2 9694
Affinity Capture-MS Homo sapiens
42 DNAJC9 23234
Proximity Label-MS Homo sapiens
43 AHR 196
Affinity Capture-MS Homo sapiens
44 IFI16 3428
Affinity Capture-MS Homo sapiens
45 Rrbp1  
Affinity Capture-MS Mus musculus
46 SERBP1 26135
Affinity Capture-MS Homo sapiens
47 PRC1 9055
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
48 FAM74A4  
Two-hybrid Homo sapiens
49 NPM1 4869
Affinity Capture-MS Homo sapiens
50 POU5F1  
Affinity Capture-MS Homo sapiens
51 Rpl35 66489
Affinity Capture-MS Mus musculus
52 OBSL1 23363
Affinity Capture-MS Homo sapiens
53 BRD4 23476
Affinity Capture-MS Homo sapiens
54 NANOG  
Affinity Capture-MS Homo sapiens
55 EMG1 10436
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
56 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
57 RTN1 6252
Affinity Capture-MS Homo sapiens
58 BRD3 8019
Affinity Capture-MS Homo sapiens
59 CEBPA  
Protein-peptide Homo sapiens
60 SLC25A38  
Two-hybrid Homo sapiens
61 EED  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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