Gene description for EIF3H
Gene name eukaryotic translation initiation factor 3, subunit H
Gene symbol EIF3H
Other names/aliases EIF3S3
eIF3-gamma
eIF3-p40
Species Homo sapiens
 Database cross references - EIF3H
ExoCarta ExoCarta_8667
Entrez Gene 8667
HGNC 3273
MIM 603912
UniProt O15372  
 EIF3H identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for EIF3H
Molecular Function
    translation initiation factor activity GO:0003743 IDA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    translation GO:0006412 TAS
    formation of translation preinitiation complex GO:0001731 IEA
    gene expression GO:0010467 TAS
    cellular protein metabolic process GO:0044267 TAS
    regulation of translational initiation GO:0006446 TAS
    translational initiation GO:0006413 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    eukaryotic translation initiation factor 3 complex, eIF3m GO:0071541 IEA
    membrane GO:0016020 IDA
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
    cytosol GO:0005829 TAS
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
 Experiment description of studies that identified EIF3H in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for EIF3H
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4A2 1974
Affinity Capture-MS Homo sapiens
2 DIPA  
Two-hybrid Homo sapiens
3 EIF3A 8661
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
4 DISC1 27185
Two-hybrid Homo sapiens
5 EIF3G 8666
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
6 ABI3 51225
Two-hybrid Homo sapiens
7 GC20  
Affinity Capture-MS Homo sapiens
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