Gene description for ARIH1
Gene name ariadne RBR E3 ubiquitin protein ligase 1
Gene symbol ARIH1
Other names/aliases ARI
HARI
HHARI
UBCH7BP
Species Homo sapiens
 Database cross references - ARIH1
ExoCarta ExoCarta_25820
Vesiclepedia VP_25820
Entrez Gene 25820
HGNC 689
MIM 605624
UniProt Q9Y4X5  
 ARIH1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ARIH1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    ubiquitin-like protein transferase activity GO:0019787 TAS
    ubiquitin conjugating enzyme binding GO:0031624 IBA
    ubiquitin conjugating enzyme binding GO:0031624 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IEA
    PKR/eIFalpha signaling GO:0039585 TAS
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IBA
    ubiquitin ligase complex GO:0000151 TAS
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    Cajal body GO:0015030 IEA
    nuclear body GO:0016604 IDA
    SCF ubiquitin ligase complex GO:0019005 IDA
    Cul2-RING ubiquitin ligase complex GO:0031462 IDA
    Cul2-RING ubiquitin ligase complex GO:0031462 IDA
    Cul3-RING ubiquitin ligase complex GO:0031463 IDA
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 IDA
    Lewy body GO:0097413 IDA
 Experiment description of studies that identified ARIH1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARIH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TNFAIP3 7128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 SNRPE 6635
Affinity Capture-MS Homo sapiens
6 ATF7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 KCTD10 83892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ZPR1 8882
Co-fractionation Homo sapiens
9 EN1  
Affinity Capture-MS Homo sapiens
10 RHOBTB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 JUND 3727
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SLURP1  
Affinity Capture-MS Homo sapiens
13 CTGF 1490
Affinity Capture-MS Homo sapiens
14 ZNF623  
Affinity Capture-MS Homo sapiens
15 HOXB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ANKMY2 57037
Co-fractionation Homo sapiens
17 FBXO3  
Affinity Capture-MS Homo sapiens
18 UBE2E2  
Reconstituted Complex Homo sapiens
19 FBXW7  
Affinity Capture-MS Homo sapiens
20 HOXA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CREB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FBLN2 2199
Affinity Capture-MS Homo sapiens
23 SYT2  
Affinity Capture-MS Homo sapiens
24 SRP14 6727
Affinity Capture-MS Homo sapiens
25 SPANXN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TCEANC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 ZNF35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 P3H1 64175
Co-fractionation Homo sapiens
30 CUL1 8454
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RBM42  
Affinity Capture-MS Homo sapiens
33 KCTD13 253980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 GSK3B 2932
Affinity Capture-MS Homo sapiens
35 CASK 8573
Affinity Capture-Western Homo sapiens
36 SRBD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 SRP9 6726
Affinity Capture-MS Homo sapiens
38 ZIC2  
Affinity Capture-MS Homo sapiens
39 RBX1 9978
Affinity Capture-MS Homo sapiens
40 KLHDC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PARK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
42 BTF3L4 91408
Co-fractionation Homo sapiens
43 SIM2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 ATF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ZNF248  
Affinity Capture-MS Homo sapiens
46 ZFP41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CTSC 1075
Co-fractionation Homo sapiens
48 VCP 7415
Affinity Capture-MS Homo sapiens
49 TRIM21 6737
Co-fractionation Homo sapiens
50 GOPC 57120
Co-fractionation Homo sapiens
51 TGIF2LY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 UBE2D3 7323
Reconstituted Complex Homo sapiens
53 VAV2 7410
Two-hybrid Homo sapiens
54 CUL2 8453
Affinity Capture-Western Homo sapiens
55 ISL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 KCTD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PRPF31 26121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 HAUS7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 SETD7 80854
Affinity Capture-MS Homo sapiens
60 MAGEE2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CT45A5  
Affinity Capture-MS Homo sapiens
62 C11orf31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 CBFB 865
Affinity Capture-MS Homo sapiens
64 CSNK1G3 1456
Affinity Capture-MS Homo sapiens
65 UBE2N 7334
Affinity Capture-Western Homo sapiens
66 IRF9 10379
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 ZNF484  
Affinity Capture-MS Homo sapiens
68 THYN1 29087
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ZNF852  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 KCTD9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 ECT2 1894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 UBE2L3 7332
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 UBE2L6 9246
Affinity Capture-Western Homo sapiens
74 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
75 SNCAIP  
Affinity Capture-Western Homo sapiens
76 ELAC2 60528
Co-fractionation Homo sapiens
77 FBXO6 26270
Affinity Capture-MS Homo sapiens
78 BBX 56987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 BATF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 RNF138  
Affinity Capture-MS Homo sapiens
82 PUS7L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 BIRC6 57448
Biochemical Activity Homo sapiens
84 PCBP1 5093
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
85 UBA2 10054
Co-fractionation Homo sapiens
86 DKC1 1736
Affinity Capture-MS Homo sapiens
87 RPA3 6119
Proximity Label-MS Homo sapiens
88 EPN3 55040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 DHPS 1725
Co-fractionation Homo sapiens
90 CRAMP1L 57585
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 ZNF687  
Affinity Capture-MS Homo sapiens
92 JUNB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 TCEB2 6923
Affinity Capture-MS Homo sapiens
94 EIF4E2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
95 ZNF263  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 PHGDH 26227
Affinity Capture-MS Homo sapiens
97 EIF3H 8667
Co-fractionation Homo sapiens
98 UBE2D1 7321
Reconstituted Complex Homo sapiens
99 U2SURP 23350
Co-fractionation Homo sapiens
100 ZRANB1 54764
Affinity Capture-MS Homo sapiens
101 PIP5K1A 8394
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 SHKBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 FBXL15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 FBXW2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 SPOP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 WDR4 10785
Co-fractionation Homo sapiens
108 PDCD2L 84306
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 TNFAIP1 7126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 KLHDC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 DR1  
Co-fractionation Homo sapiens
112 PLCD3 113026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 JUN 3725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 SKP1 6500
Affinity Capture-MS Homo sapiens
115 FBXL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 HSPB1 3315
Two-hybrid Homo sapiens
117 TRIM9  
Affinity Capture-MS Homo sapiens
118 KBTBD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 KATNA1 11104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 KLHL7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 DDX58 23586
Affinity Capture-RNA Homo sapiens
122 ATF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 CREB5 9586
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 ZNF501  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 XPO5 57510
Co-fractionation Homo sapiens
126 HOXC9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 TEFM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 ZFP82  
Affinity Capture-MS Homo sapiens
129 CUL4A 8451
Affinity Capture-Western Homo sapiens
130 NDEL1 81565
Affinity Capture-MS Homo sapiens
131 KRAS 3845
Negative Genetic Homo sapiens
132 UHRF1 29128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 ISL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 DDX47 51202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 NOTCH1 4851
Affinity Capture-MS Homo sapiens
136 LRRTM3  
Affinity Capture-MS Homo sapiens
137 NEDD8 4738
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
138 ARIH1 25820
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
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