Gene description for NCBP1
Gene name nuclear cap binding protein subunit 1, 80kDa
Gene symbol NCBP1
Other names/aliases CBP80
NCBP
Sto1
Species Homo sapiens
 Database cross references - NCBP1
ExoCarta ExoCarta_4686
Entrez Gene 4686
HGNC 7658
MIM 600469
UniProt Q09161  
 NCBP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for NCBP1
Molecular Function
    RNA binding GO:0003723 TAS
    RNA cap binding GO:0000339 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    positive regulation of mRNA 3'-end processing GO:0031442 IDA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    gene expression GO:0010467 TAS
    mRNA cleavage GO:0006379 IDA
    7-methylguanosine mRNA capping GO:0006370 TAS
    spliceosomal snRNP assembly GO:0000387 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    mRNA export from nucleus GO:0006406 TAS
    viral process GO:0016032 TAS
    gene silencing by RNA GO:0031047 IEA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    RNA splicing GO:0008380 TAS
    mRNA cis splicing, via spliceosome GO:0045292 IEA
    histone mRNA metabolic process GO:0008334 TAS
    ncRNA metabolic process GO:0034660 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    mRNA 3'-end processing GO:0031124 TAS
    positive regulation of viral transcription GO:0050434 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    regulation of translational initiation GO:0006446 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
    mitochondrion GO:0005739 IDA
    cytoplasm GO:0005737 IDA
    mRNA cap binding complex GO:0005845 IDA
    nucleus GO:0005634 IDA
    nuclear cap binding complex GO:0005846 IEA
    ribonucleoprotein complex GO:0030529 IDA
 Experiment description of studies that identified NCBP1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
5
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Reconstituted Complex Homo sapiens
2 EIF4G1 1981
Invivo Homo sapiens
3 BNIP1  
Two-hybrid Homo sapiens
4 RNF40 9810
Two-hybrid Homo sapiens
5 NCBP2 22916
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
6 BNIP3  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 HNRNPF 3185
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
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