Gene description for ACTN3
Gene name actinin, alpha 3 (gene/pseudogene)
Gene symbol ACTN3
Other names/aliases -
Species Homo sapiens
 Database cross references - ACTN3
ExoCarta ExoCarta_89
Entrez Gene 89
HGNC 165
MIM 102574
UniProt Q08043  
 ACTN3 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ACTN3
Molecular Function
    protein homodimerization activity GO:0042803 TAS
    integrin binding GO:0005178 TAS
    actin binding GO:0003779 IEA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    structural constituent of muscle GO:0008307 TAS
Biological Process
    focal adhesion assembly GO:0048041 IMP
    regulation of apoptotic process GO:0042981 NAS
    muscle filament sliding GO:0030049 TAS
Subcellular Localization
    actin filament GO:0005884 TAS
    extracellular exosome GO:0070062 IDA
    focal adhesion GO:0005925 IMP
    cytosol GO:0005829 TAS
    pseudopodium GO:0031143 TAS
 Experiment description of studies that identified ACTN3 in exosomes
1
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACTN3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTN3 89
Invivo Homo sapiens
Invivo Homo sapiens
2 ACTN2 88
Invivo Homo sapiens
Invitro Homo sapiens
3 MYPN 84665
Two-hybrid Homo sapiens
4 ACTN4 81
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which ACTN3 is involved
PathwayEvidenceSource
Nephrin interactions TAS Reactome
Striated Muscle Contraction TAS Reactome





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