Gene description for SPTLC1
Gene name serine palmitoyltransferase, long chain base subunit 1
Gene symbol SPTLC1
Other names/aliases HSAN1
HSN1
LBC1
LCB1
SPT1
SPTI
Species Homo sapiens
 Database cross references - SPTLC1
ExoCarta ExoCarta_10558
Entrez Gene 10558
HGNC 11277
MIM 605712
UniProt O15269  
 SPTLC1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SPTLC1
Molecular Function
    pyridoxal phosphate binding GO:0030170 IEA
    protein binding GO:0005515 IPI
    serine C-palmitoyltransferase activity GO:0004758 IDA
Biological Process
    ceramide biosynthetic process GO:0046513 IEA
    sphingomyelin biosynthetic process GO:0006686 IEA
    sphingolipid metabolic process GO:0006665 TAS
    sphingosine biosynthetic process GO:0046512 IEA
    sphinganine biosynthetic process GO:0046511 IEA
    sphingolipid biosynthetic process GO:0030148 TAS
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
    serine C-palmitoyltransferase complex GO:0017059 IDA
    SPOTS complex GO:0035339 IDA
 Experiment description of studies that identified SPTLC1 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPTLC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RGS20 8601
Affinity Capture-MS Homo sapiens
2 MAGED1  
Affinity Capture-MS Homo sapiens
3 C20orf24  
Affinity Capture-MS Homo sapiens
4 SPTLC2 9517
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which SPTLC1 is involved
PathwayEvidenceSource
Sphingolipid de novo biosynthesis TAS Reactome





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