Gene description for LIMA1
Gene name LIM domain and actin binding 1
Gene symbol LIMA1
Other names/aliases EPLIN
SREBP3
Species Homo sapiens
 Database cross references - LIMA1
ExoCarta ExoCarta_51474
Entrez Gene 51474
HGNC 24636
MIM 608364
UniProt Q9UHB6  
 LIMA1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for LIMA1
Molecular Function
    actin monomer binding GO:0003785 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    actin filament binding GO:0051015 IDA
Biological Process
    actin filament bundle assembly GO:0051017 IDA
    ruffle organization GO:0031529 IDA
    negative regulation of actin filament depolymerization GO:0030835 IDA
Subcellular Localization
    focal adhesion GO:0005925 IDA
    cytoplasm GO:0005737 IDA
    actin cytoskeleton GO:0015629 IDA
    plasma membrane GO:0005886 IDA
    cleavage furrow GO:0032154 IDA
    stress fiber GO:0001725 IDA
 Experiment description of studies that identified LIMA1 in exosomes
1
Experiment ID 20
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
CD63|CD81|LAMP1
EV Membrane markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LIMA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which LIMA1 is involved
No pathways found





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