Gene description for PRKCZ
Gene name protein kinase C, zeta
Gene symbol PRKCZ
Other names/aliases PKC-ZETA
PKC2
Species Homo sapiens
 Database cross references - PRKCZ
ExoCarta ExoCarta_5590
Entrez Gene 5590
HGNC 9412
MIM 176982
UniProt Q05513  
 PRKCZ identified in exosomes derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for PRKCZ
Molecular Function
    protein kinase C activity GO:0004697 IEA
    insulin receptor substrate binding GO:0043560 IC
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IEA
    phospholipase binding GO:0043274 IEA
    potassium channel regulator activity GO:0015459 IEA
    ATP binding GO:0005524 IEA
    protein domain specific binding GO:0019904 IEA
    14-3-3 protein binding GO:0071889 IEA
    metal ion binding GO:0046872 IEA
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IDA
Biological Process
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    platelet activation GO:0030168 TAS
    negative regulation of protein complex assembly GO:0031333 IMP
    negative regulation of hydrolase activity GO:0051346 IEA
    inflammatory response GO:0006954 IEA
    protein heterooligomerization GO:0051291 IEA
    activation of phospholipase D activity GO:0031584 IEA
    establishment of cell polarity GO:0030010 ISS
    peptidyl-serine phosphorylation GO:0018105 IDA
    positive regulation of interleukin-13 secretion GO:2000667 ISS
    protein localization to plasma membrane GO:0072659 IEA
    activation of protein kinase B activity GO:0032148 IEA
    protein phosphorylation GO:0006468 IDA
    protein kinase C signaling GO:0070528 IEA
    positive regulation of excitatory postsynaptic membrane potential GO:2000463 ISS
    positive regulation of cell-matrix adhesion GO:0001954 IEA
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 IMP
    positive regulation of interleukin-10 secretion GO:2001181 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    positive regulation of interleukin-4 production GO:0032753 ISS
    signal transduction GO:0007165 TAS
    negative regulation of apoptotic process GO:0043066 IEA
    membrane hyperpolarization GO:0060081 IEA
    positive regulation of insulin receptor signaling pathway GO:0046628 ISS
    negative regulation of insulin receptor signaling pathway GO:0046627 IMP
    positive regulation of T-helper 2 cell differentiation GO:0045630 ISS
    positive regulation of interleukin-5 secretion GO:2000664 ISS
    actin cytoskeleton reorganization GO:0031532 IEA
    vesicle transport along microtubule GO:0047496 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    positive regulation of glucose import GO:0046326 IEA
    insulin receptor signaling pathway GO:0008286 IEA
    positive regulation of T-helper 2 cell cytokine production GO:2000553 ISS
    microtubule cytoskeleton organization GO:0000226 IEA
    cell migration GO:0016477 IEA
    long-term memory GO:0007616 IEA
    positive regulation of cell proliferation GO:0008284 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    long-term synaptic potentiation GO:0060291 ISS
    neuron projection extension GO:1990138 IEA
    blood coagulation GO:0007596 TAS
Subcellular Localization
    membrane raft GO:0045121 IEA
    apical plasma membrane GO:0016324 IEA
    cell leading edge GO:0031252 IEA
    filamentous actin GO:0031941 IEA
    cell-cell junction GO:0005911 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    endosome GO:0005768 IEA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    extracellular exosome GO:0070062 IDA
    axon hillock GO:0043203 IEA
    nuclear envelope GO:0005635 IEA
    myelin sheath abaxonal region GO:0035748 IEA
    bicellular tight junction GO:0005923 IEA
    cytosol GO:0005829 TAS
    nuclear matrix GO:0016363 IEA
    microtubule organizing center GO:0005815 IEA
    cytoplasm GO:0005737 TAS
    cell junction GO:0030054 TAS
    apical cortex GO:0045179 IEA
 Experiment description of studies that identified PRKCZ in exosomes
1
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PRKCZ
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FEZ1  
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 PDLIM7 9260
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 PPP3CA 5530
Affinity Capture-MS Homo sapiens
4 FADD 8772
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 DAPK3  
Reconstituted Complex Homo sapiens
6 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 CSNK2B 1460
Affinity Capture-Western Homo sapiens
8 YWHAB 7529
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
9 ECT2 1894
Affinity Capture-Western Homo sapiens
10 KRT10 3858
Affinity Capture-Western Homo sapiens
11 PAWR  
Invitro Homo sapiens
Invivo Homo sapiens
12 IRS4 8471
Invitro Homo sapiens
13 AKT3  
Invitro Homo sapiens
Invivo Homo sapiens
14 YWHAZ 7534
Reconstituted Complex Homo sapiens
15 SQSTM1 8878
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
16 RPS6KB1  
Affinity Capture-Western Homo sapiens
17 IRAK1  
Affinity Capture-Western Homo sapiens
18 MAP2K5  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
19 PARD6G  
Invitro Homo sapiens
Two-hybrid Homo sapiens
20 PDPK1  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
21 GRB14  
Affinity Capture-Western Homo sapiens
22 FEZ2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 RELA  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
24 SLC39A2  
Invitro Homo sapiens
25 AFAP1 60312
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
26 NCL 4691
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
27 KIBRA  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
28 C1QBP 708
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
29 RAF1 5894
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
30 CENTA1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
31 SRC 6714
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
32 AKT1 207
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
33 PARD6A  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
34 NFATC2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
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