Gene description for RBM14
Gene name RNA binding motif protein 14
Gene symbol RBM14
Other names/aliases COAA
PSP2
SIP
SYTIP1
TMEM137
Species Homo sapiens
 Database cross references - RBM14
ExoCarta ExoCarta_10432
Entrez Gene 10432
HGNC 14219
MIM 612409
UniProt Q96PK6  
 RBM14 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Thymus 23844026    
 Gene ontology annotations for RBM14
Molecular Function
    RNA polymerase II transcription cofactor activity GO:0001104 NAS
    nucleotide binding GO:0000166 IEA
    protein binding, bridging GO:0030674 NAS
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 IPI
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
    RNA binding GO:0003723 NAS
Biological Process
    histone deacetylation GO:0016575 IPI
    transcription from RNA polymerase II promoter GO:0006366 NAS
    DNA recombination GO:0006310 NAS
    glucocorticoid receptor signaling pathway GO:0042921 NAS
    intracellular estrogen receptor signaling pathway GO:0030520 NAS
    response to hormone GO:0009725 TAS
    DNA replication GO:0006260 NAS
    DNA repair GO:0006281 NAS
    negative regulation of centriole replication GO:0046600 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
Subcellular Localization
    mediator complex GO:0016592 NAS
    nucleoplasm GO:0005654 IDA
    ribonucleoprotein complex GO:0030529 TAS
    transcription factor complex GO:0005667 IPI
    nucleolus GO:0005730 IEA
 Experiment description of studies that identified RBM14 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RBM14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PARP1 142
Affinity Capture-Western Homo sapiens
2 TARBP2  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 EP300 2033
Reconstituted Complex Homo sapiens
4 XRCC5 7520
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which RBM14 is involved
No pathways found





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