Gene description for TOP2B
Gene name topoisomerase (DNA) II beta 180kDa
Gene symbol TOP2B
Other names/aliases TOPIIB
top2beta
Species Homo sapiens
 Database cross references - TOP2B
ExoCarta ExoCarta_7155
Vesiclepedia VP_7155
Entrez Gene 7155
HGNC 11990
MIM 126431
UniProt Q02880  
 TOP2B identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for TOP2B
Molecular Function
    DNA binding GO:0003677 IEA
    chromatin binding GO:0003682 IDA
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 IDA
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    DNA negative supercoiling activity GO:0034335 IEA
    ribonucleoprotein complex binding GO:0043021 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    resolution of meiotic recombination intermediates GO:0000712 IBA
    sister chromatid segregation GO:0000819 IBA
    neuron migration GO:0001764 IEA
    DNA topological change GO:0006265 IDA
    axonogenesis GO:0007409 IEA
    B cell differentiation GO:0030183 IMP
    forebrain development GO:0030900 IEA
    positive regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045870 IMP
    cellular response to hydrogen peroxide GO:0070301 IEA
    cellular response to ATP GO:0071318 IEA
    cellular senescence GO:0090398 IEA
    positive regulation of double-strand break repair via nonhomologous end joining GO:2001034 IEA
Subcellular Localization
    heterochromatin GO:0000792 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified TOP2B in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 207
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TOP2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSD3B2 3284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 XPC  
Co-fractionation Homo sapiens
4 MCM7 4176
Co-fractionation Homo sapiens
5 ISG15 9636
Affinity Capture-MS Homo sapiens
6 CBX1 10951
Affinity Capture-MS Homo sapiens
7 HDAC2 3066
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 SNRPE 6635
Co-fractionation Homo sapiens
9 COIL  
Proximity Label-MS Homo sapiens
10 DNAJC8 22826
Proximity Label-MS Homo sapiens
11 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
12 FOXD3  
Affinity Capture-MS Homo sapiens
13 RPA2 6118
Affinity Capture-MS Homo sapiens
14 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
15 NOP56 10528
Proximity Label-MS Homo sapiens
16 SMARCE1 6605
Co-fractionation Homo sapiens
17 KIF20A 10112
Affinity Capture-MS Homo sapiens
18 BRD2  
Affinity Capture-MS Homo sapiens
19 HNRNPR 10236
Co-fractionation Homo sapiens
20 MED24 9862
Affinity Capture-MS Homo sapiens
21 SMARCD2 6603
Co-fractionation Homo sapiens
22 SENP3 26168
Affinity Capture-MS Homo sapiens
23 CENPA  
Proximity Label-MS Homo sapiens
24 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
25 APEX1 328
Proximity Label-MS Homo sapiens
26 HSPA4 3308
Co-fractionation Homo sapiens
27 CAPZB 832
Affinity Capture-MS Homo sapiens
28 DDX23 9416
Proximity Label-MS Homo sapiens
29 GPATCH4 54865
Affinity Capture-MS Homo sapiens
30 HDAC3 8841
Co-fractionation Homo sapiens
31 Eef1a1 13627
Affinity Capture-MS Mus musculus
32 SSRP1 6749
Affinity Capture-MS Homo sapiens
33 KIF23 9493
Affinity Capture-MS Homo sapiens
34 FBXW7  
Affinity Capture-MS Homo sapiens
35 PRC1 9055
Affinity Capture-MS Homo sapiens
36 CALD1 800
Affinity Capture-MS Homo sapiens
37 NPM1 4869
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
38 FBL 2091
Proximity Label-MS Homo sapiens
39 POLR1E  
Proximity Label-MS Homo sapiens
40 OBSL1 23363
Affinity Capture-MS Homo sapiens
41 HMGA1 3159
Affinity Capture-MS Homo sapiens
42 ZNF451 26036
Affinity Capture-MS Homo sapiens
43 CD3E 916
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
44 BRD4 23476
Affinity Capture-MS Homo sapiens
45 ATG16L1 55054
Affinity Capture-MS Homo sapiens
46 KLHL20  
Affinity Capture-MS Homo sapiens
47 MECP2 4204
Affinity Capture-MS Homo sapiens
48 MED27  
Affinity Capture-MS Homo sapiens
49 CSNK2B 1460
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
50 SMARCA2 6595
Co-fractionation Homo sapiens
51 NCSTN 23385
Co-fractionation Homo sapiens
52 MTA2 9219
Affinity Capture-Western Homo sapiens
53 RBM14 10432
Co-fractionation Homo sapiens
54 CTCF  
Affinity Capture-Western Homo sapiens
55 KIF14 9928
Affinity Capture-MS Homo sapiens
56 UNK  
Affinity Capture-RNA Homo sapiens
57 CHMP4C 92421
Affinity Capture-MS Homo sapiens
58 H2AFZ 3015
Affinity Capture-MS Homo sapiens
59 RPL27 6155
Cross-Linking-MS (XL-MS) Homo sapiens
60 SRP9 6726
Affinity Capture-MS Homo sapiens
61 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 ATP5A1 498
Cross-Linking-MS (XL-MS) Homo sapiens
63 FOXC1  
Affinity Capture-MS Homo sapiens
64 MYH10 4628
Co-fractionation Homo sapiens
65 COX15 1355
Affinity Capture-MS Homo sapiens
66 PIAS4  
Affinity Capture-MS Homo sapiens
67 LYN 4067
Proximity Label-MS Homo sapiens
68 RPL5 6125
Affinity Capture-MS Homo sapiens
69 CBX3 11335
Proximity Label-MS Homo sapiens
70 SNRNP200 23020
Co-fractionation Homo sapiens
71 VRK1 7443
Affinity Capture-MS Homo sapiens
72 UBE2I 7329
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
73 SMARCA4 6597
Co-fractionation Homo sapiens
74 MKRN2 23609
Affinity Capture-RNA Homo sapiens
75 BARD1 580
Affinity Capture-Western Homo sapiens
76 NTRK1 4914
Affinity Capture-MS Homo sapiens
77 RPL31 6160
Proximity Label-MS Homo sapiens
78 ACTL6A 86
Co-fractionation Homo sapiens
79 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
80 RAD18  
Affinity Capture-MS Homo sapiens
81 SUMO2 6613
Reconstituted Complex Homo sapiens
82 PML 5371
Affinity Capture-MS Homo sapiens
83 HECTD1 25831
Affinity Capture-MS Homo sapiens
84 ENY2 56943
Cross-Linking-MS (XL-MS) Homo sapiens
85 CTR9 9646
Cross-Linking-MS (XL-MS) Homo sapiens
86 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
87 RPS16 6217
Affinity Capture-MS Homo sapiens
88 NECAP1 25977
Affinity Capture-MS Homo sapiens
89 CEBPA  
Protein-peptide Homo sapiens
90 DAXX  
Affinity Capture-MS Homo sapiens
91 PIPSL 266971
Affinity Capture-MS Homo sapiens
92 BRCA1 672
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
93 MED15  
Affinity Capture-MS Homo sapiens
94 SMARCC2 6601
Co-fractionation Homo sapiens
95 CD3EAP  
Proximity Label-MS Homo sapiens
96 SCARNA22  
Affinity Capture-RNA Homo sapiens
97 ANLN 54443
Affinity Capture-MS Homo sapiens
98 NUMA1 4926
Affinity Capture-MS Homo sapiens
99 SFPQ 6421
Co-fractionation Homo sapiens
100 ZBTB2 57621
Affinity Capture-MS Homo sapiens
101 TAF15 8148
Affinity Capture-MS Homo sapiens
102 DNAJC21  
Affinity Capture-MS Homo sapiens
103 HIST1H3A 8350
Proximity Label-MS Homo sapiens
104 FANCD2  
Affinity Capture-MS Homo sapiens
105 RPL10 6134
Affinity Capture-MS Homo sapiens
106 MSH2 4436
Co-fractionation Homo sapiens
107 BAZ1B 9031
Co-fractionation Homo sapiens
108 EIF6 3692
Affinity Capture-MS Homo sapiens
109 ECT2 1894
Affinity Capture-MS Homo sapiens
110 RPL35 11224
Cross-Linking-MS (XL-MS) Homo sapiens
111 RPS6 6194
Proximity Label-MS Homo sapiens
112 LAMTOR5 10542
Two-hybrid Homo sapiens
113 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
114 FKBP5 2289
Affinity Capture-MS Homo sapiens
115 PSPC1 55269
Affinity Capture-MS Homo sapiens
116 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
117 DLST 1743
Affinity Capture-MS Homo sapiens
118 TOP1 7150
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
119 SMARCB1 6598
Co-fractionation Homo sapiens
120 HNRNPAB 3182
Cross-Linking-MS (XL-MS) Homo sapiens
121 DNAH2 146754
Cross-Linking-MS (XL-MS) Homo sapiens
122 ARID1A 8289
Co-fractionation Homo sapiens
123 SEPT7 989
Co-fractionation Homo sapiens
124 Eif3e 16341
Affinity Capture-MS Mus musculus
125 LMNA 4000
Proximity Label-MS Homo sapiens
126 BRD3 8019
Affinity Capture-MS Homo sapiens
127 SUPT16H 11198
Co-fractionation Homo sapiens
128 SUMO1 7341
Two-hybrid Homo sapiens
129 Top2b 21974
Affinity Capture-MS Mus musculus
130 FTL 2512
Affinity Capture-MS Homo sapiens
131 Cbx1  
Affinity Capture-MS Mus musculus
132 ASB3 51130
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 SRRM1 10250
Affinity Capture-MS Homo sapiens
134 RARA 5914
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
135 SIRT6  
Affinity Capture-MS Homo sapiens
136 PARP1 142
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
137 TOPBP1 11073
Two-hybrid Homo sapiens
138 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
139 TRIM52  
Affinity Capture-MS Homo sapiens
140 SMARCC1 6599
Co-fractionation Homo sapiens
141 RPA1 6117
Affinity Capture-MS Homo sapiens
142 MEN1 4221
Affinity Capture-MS Homo sapiens
143 POLE3  
Co-fractionation Homo sapiens
144 CUL5 8065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
146 HMGN5 79366
Affinity Capture-MS Homo sapiens
147 Atrx  
Affinity Capture-MS Mus musculus
148 HDAC1 3065
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
149 CHD4 1108
Affinity Capture-MS Homo sapiens
150 G3BP2 9908
Affinity Capture-MS Homo sapiens
151 S100A9 6280
Co-fractionation Homo sapiens
152 NIFK 84365
Proximity Label-MS Homo sapiens
153 TOP2A 7153
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
154 ESR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
155 IK 3550
Co-fractionation Homo sapiens
156 RPS24 6229
Proximity Label-MS Homo sapiens
157 NCL 4691
Co-fractionation Homo sapiens
158 Rrbp1  
Affinity Capture-MS Mus musculus
159 GLYR1 84656
Co-fractionation Homo sapiens
160 ERG  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
161 PTGES3 10728
Affinity Capture-MS Homo sapiens
162 CUL7 9820
Affinity Capture-MS Homo sapiens
163 USF1  
Affinity Capture-Western Homo sapiens
164 NUCKS1 64710
Affinity Capture-MS Homo sapiens
165 NOTCH1 4851
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
166 RANBP2 5903
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
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