Gene description for SPECC1
Gene name sperm antigen with calponin homology and coiled-coil domains 1
Gene symbol SPECC1
Other names/aliases CYTSB
HCMOGT-1
HCMOGT1
NSP
Species Homo sapiens
 Database cross references - SPECC1
ExoCarta ExoCarta_92521
Vesiclepedia VP_92521
Entrez Gene 92521
HGNC 30615
MIM 608793
UniProt Q5M775  
 SPECC1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SPECC1
Biological Process
    blastocyst development GO:0001824 IEA
    associative learning GO:0008306 IEA
    actin cytoskeleton organization GO:0030036 IBA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleoplasm GO:0005654 IDA
    microtubule organizing center GO:0005815 IBA
    cytosol GO:0005829 IDA
    membrane GO:0016020 IEA
    filamentous actin GO:0031941 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    apical part of cell GO:0045177 IEA
 Experiment description of studies that identified SPECC1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SPECC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ACTR2 10097
Affinity Capture-MS Homo sapiens
3 KRT37 8688
Affinity Capture-MS Homo sapiens
4 Cdk1 12534
Affinity Capture-MS Mus musculus
5 PPP1CB 5500
Affinity Capture-MS Homo sapiens
6 OCLN 100506658
Proximity Label-MS Homo sapiens
7 PCDHA8  
Affinity Capture-MS Homo sapiens
8 LAMP3  
Proximity Label-MS Homo sapiens
9 PCDHGB5 56101
Affinity Capture-MS Homo sapiens
10 PPIE 10450
Affinity Capture-MS Homo sapiens
11 BTF3 689
Affinity Capture-MS Homo sapiens
12 NUP62 23636
Affinity Capture-MS Homo sapiens
13 Calml3  
Affinity Capture-MS Mus musculus
14 DBN1 1627
Affinity Capture-MS Homo sapiens
15 KIF20A 10112
Affinity Capture-MS Homo sapiens
16 LAMP2 3920
Proximity Label-MS Homo sapiens
17 MYO5C 55930
Affinity Capture-MS Homo sapiens
18 Actb 11461
Affinity Capture-MS Mus musculus
19 STX4 6810
Proximity Label-MS Homo sapiens
20 SEPT9 10801
Proximity Label-MS Homo sapiens
21 DPPA4  
Affinity Capture-MS Homo sapiens
22 CAPZB 832
Affinity Capture-MS Homo sapiens
23 MLLT4 4301
Proximity Label-MS Homo sapiens
24 Trim69  
Affinity Capture-MS Mus musculus
25 KIF23 9493
Affinity Capture-MS Homo sapiens
26 CALD1 800
Affinity Capture-MS Homo sapiens
27 Poc1b  
Affinity Capture-MS Mus musculus
28 PRC1 9055
Affinity Capture-MS Homo sapiens
29 IQGAP1 8826
Affinity Capture-MS Homo sapiens
30 CALM3 808
Affinity Capture-MS Homo sapiens
31 POU5F1  
Affinity Capture-MS Homo sapiens
32 Flot2 14252
Affinity Capture-MS Mus musculus
33 KRT31 3881
Affinity Capture-MS Homo sapiens
34 Tpm1 22003
Affinity Capture-MS Mus musculus
35 ATG16L1 55054
Affinity Capture-MS Homo sapiens
36 RAB35 11021
Proximity Label-MS Homo sapiens
37 WDR5 11091
Affinity Capture-MS Homo sapiens
38 Erh  
Affinity Capture-MS Mus musculus
39 Lima1  
Affinity Capture-MS Mus musculus
40 LAMTOR1 55004
Proximity Label-MS Homo sapiens
41 Ppp1cb 19046
Affinity Capture-MS Mus musculus
42 MYO18A 399687
Affinity Capture-MS Homo sapiens
43 GJA1 2697
Proximity Label-MS Homo sapiens
44 LYN 4067
Proximity Label-MS Homo sapiens
45 MAPRE1 22919
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PCDHB11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 RAB11FIP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CDH1 999
Proximity Label-MS Homo sapiens
49 CHMP4B 128866
Affinity Capture-MS Homo sapiens
50 NELFE 7936
Affinity Capture-MS Homo sapiens
51 DIRAS3  
Proximity Label-MS Homo sapiens
52 Stag2  
Affinity Capture-MS Mus musculus
53 DCTN1 1639
Proximity Label-MS Homo sapiens
54 MYH10 4628
Affinity Capture-MS Homo sapiens
55 Coro1c 23790
Affinity Capture-MS Mus musculus
56 MYH9 4627
Affinity Capture-MS Homo sapiens
57 DAXX  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 LIMA1 51474
Affinity Capture-MS Homo sapiens
60 SLX4  
Affinity Capture-MS Homo sapiens
61 PCDHGB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 Myh9 17886
Affinity Capture-MS Mus musculus
63 RAB5A 5868
Affinity Capture-MS Homo sapiens
64 ECT2 1894
Affinity Capture-MS Homo sapiens
65 CTNNB1 1499
Affinity Capture-MS Homo sapiens
66 Itga5 16402
Affinity Capture-MS Mus musculus
67 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
68 YWHAZ 7534
Affinity Capture-MS Homo sapiens
69 CTTN 2017
Affinity Capture-MS Homo sapiens
70 MYO19  
Affinity Capture-MS Homo sapiens
71 TMEM74  
Affinity Capture-MS Homo sapiens
72 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 LARS 51520
Affinity Capture-MS Homo sapiens
74 MYO1C 4641
Affinity Capture-MS Homo sapiens
75 NXF1 10482
Affinity Capture-RNA Homo sapiens
76 MAPRE3  
Proximity Label-MS Homo sapiens
77 PCDHGB2  
Affinity Capture-MS Homo sapiens
78 SYNPO 11346
Affinity Capture-MS Homo sapiens
79 Flnb 286940
Affinity Capture-MS Mus musculus
80 MYC  
Affinity Capture-MS Homo sapiens
81 HECW2  
Affinity Capture-MS Homo sapiens
82 RAB9A 9367
Proximity Label-MS Homo sapiens
83 PCGF1 84759
Affinity Capture-MS Homo sapiens
84 DDRGK1 65992
Affinity Capture-MS Homo sapiens
85 RNF2  
Affinity Capture-MS Homo sapiens
86 RHOB 388
Proximity Label-MS Homo sapiens
87 NANOG  
Affinity Capture-MS Homo sapiens
88 RBBP5 5929
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 CCDC8  
Affinity Capture-MS Homo sapiens
90 TMEM169  
Affinity Capture-MS Homo sapiens
91 ANKFY1 51479
Proximity Label-MS Homo sapiens
92 Tmod3 50875
Affinity Capture-MS Mus musculus
93 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 KIF14 9928
Affinity Capture-MS Homo sapiens
95 BKRF1  
Affinity Capture-MS
96 PPP2CA 5515
Affinity Capture-MS Homo sapiens
97 Myo1c 17913
Affinity Capture-MS Mus musculus
98 CAPZA2 830
Affinity Capture-MS Homo sapiens
99 CDH23 64072
Affinity Capture-MS Homo sapiens
100 C11orf52 91894
Proximity Label-MS Homo sapiens
101 Myh10 77579
Affinity Capture-MS Mus musculus
102 FLNA 2316
Affinity Capture-MS Homo sapiens
103 PINK1  
Affinity Capture-MS Homo sapiens
104 RAB11A 8766
Proximity Label-MS Homo sapiens
105 EPHA2 1969
Proximity Label-MS Homo sapiens
106 KRAS 3845
Proximity Label-MS Homo sapiens
107 Sept9  
Affinity Capture-MS Mus musculus
108 CAV1 857
Proximity Label-MS Homo sapiens
109 Tpm4  
Affinity Capture-MS Mus musculus
110 Mau2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which SPECC1 is involved
No pathways found





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