Gene description for MYL6B
Gene name myosin, light chain 6B, alkali, smooth muscle and non-muscle
Gene symbol MYL6B
Other names/aliases MLC1SA
Species Homo sapiens
 Database cross references - MYL6B
ExoCarta ExoCarta_140465
Vesiclepedia VP_140465
Entrez Gene 140465
HGNC 29823
MIM 609930
UniProt P14649  
 MYL6B identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
 Gene ontology annotations for MYL6B
Molecular Function
    cytoskeletal motor activity GO:0003774 TAS
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    structural constituent of muscle GO:0008307 IBA
    structural constituent of muscle GO:0008307 TAS
Biological Process
    muscle contraction GO:0006936 TAS
    skeletal muscle tissue development GO:0007519 TAS
    muscle filament sliding GO:0030049 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    muscle myosin complex GO:0005859 TAS
    myosin complex GO:0016459 TAS
    myosin II complex GO:0016460 IBA
    unconventional myosin complex GO:0016461 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MYL6B in exosomes
1
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 20
MISEV standards
EM
Biophysical techniques
HSP90|CD63|CD81|LAMP1
Enriched markers
GOLGA2|cytochrome c
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
4
Experiment ID 21
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101|HSP70|CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for MYL6B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYO9A 4649
Affinity Capture-MS Homo sapiens
2 CEP57  
Affinity Capture-MS Homo sapiens
3 NET1 10276
Affinity Capture-MS Homo sapiens
4 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PDLIM7 9260
Affinity Capture-MS Homo sapiens
6 MYL12A 10627
Affinity Capture-MS Homo sapiens
7 CUL1 8454
Affinity Capture-MS Homo sapiens
8 TMPO 7112
Affinity Capture-MS Homo sapiens
9 MYCN  
Affinity Capture-MS Homo sapiens
10 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
11 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
12 SLFN11 91607
Proximity Label-MS Homo sapiens
13 Myh9 17886
Affinity Capture-MS Mus musculus
14 WDR77 79084
Affinity Capture-MS Homo sapiens
15 C11orf58  
Co-fractionation Homo sapiens
16 MYL6 4637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
17 CDC42 998
Affinity Capture-MS Homo sapiens
18 KIF20A 10112
Affinity Capture-MS Homo sapiens
19 ECT2 1894
Affinity Capture-MS Homo sapiens
20 MYL9 10398
Affinity Capture-MS Homo sapiens
21 DYNC1I2 1781
Proximity Label-MS Homo sapiens
22 CALM2 805
Affinity Capture-MS Homo sapiens
23 SP1  
Affinity Capture-MS Homo sapiens
24 RLIM 51132
Affinity Capture-MS Homo sapiens
25 Calml3  
Affinity Capture-MS Mus musculus
26 Myo1c 17913
Affinity Capture-MS Mus musculus
27 MYO18A 399687
Affinity Capture-MS Homo sapiens
28 POLD1 5424
Affinity Capture-MS Homo sapiens
29 MYH10 4628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CUL3 8452
Affinity Capture-MS Homo sapiens
31 CAPZA2 830
Affinity Capture-MS Homo sapiens
32 BTF3 689
Affinity Capture-MS Homo sapiens
33 ISG15 9636
Affinity Capture-MS Homo sapiens
34 TFCP2 7024
Affinity Capture-MS Homo sapiens
35 MYO19  
Affinity Capture-MS Homo sapiens
36 SPA17  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 Actb 11461
Affinity Capture-MS Mus musculus
38 DDX58 23586
Affinity Capture-RNA Homo sapiens
39 MYO6 4646
Affinity Capture-MS Homo sapiens
40 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 OCRL 4952
Affinity Capture-MS Homo sapiens
42 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
43 ESR1  
Affinity Capture-MS Homo sapiens
44 Myo9b  
Affinity Capture-MS Rattus norvegicus
45 NCKAP1 10787
Affinity Capture-MS Homo sapiens
46 MYL12B 103910
Affinity Capture-MS Homo sapiens
47 FOS 2353
Proximity Label-MS Homo sapiens
48 CHMP4B 128866
Affinity Capture-MS Homo sapiens
49 MYC  
Affinity Capture-MS Homo sapiens
50 YAP1 10413
Affinity Capture-MS Homo sapiens
51 MYH9 4627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 IQGAP3 128239
Affinity Capture-MS Homo sapiens
53 KIF14 9928
Affinity Capture-MS Homo sapiens
54 TCF3  
Affinity Capture-MS Homo sapiens
55 MYO9B 4650
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 STAT1 6772
Proximity Label-MS Homo sapiens
57 FBXW7  
Affinity Capture-MS Homo sapiens
58 HUWE1 10075
Affinity Capture-MS Homo sapiens
59 IQGAP1 8826
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
60 DTX3  
Affinity Capture-MS Homo sapiens
61 RNF2  
Affinity Capture-MS Homo sapiens
62 RAC1 5879
Affinity Capture-MS Homo sapiens
63 MYH14 79784
Affinity Capture-MS Homo sapiens
64 CEP76  
Affinity Capture-MS Homo sapiens
65 CALM3 808
Affinity Capture-MS Homo sapiens
66 PRC1 9055
Affinity Capture-MS Homo sapiens
67 BRD3 8019
Affinity Capture-MS Homo sapiens
68 MDM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
69 FARP1 10160
Affinity Capture-MS Homo sapiens
70 CCDC8  
Affinity Capture-MS Homo sapiens
71 Myl12a  
Affinity Capture-MS Mus musculus
72 H2AFX 3014
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which MYL6B is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Smooth Muscle Contraction TAS Reactome





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