Gene description for USP9X
Gene name ubiquitin specific peptidase 9, X-linked
Gene symbol USP9X
Other names/aliases DFFRX
FAF
FAM
MRX99
Species Homo sapiens
 Database cross references - USP9X
ExoCarta ExoCarta_8239
Vesiclepedia VP_8239
Entrez Gene 8239
HGNC 12632
MIM 300072
UniProt Q93008  
 USP9X identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for USP9X
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 ISS
    cysteine-type deubiquitinase activity GO:0004843 EXP
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 TAS
    protein binding GO:0005515 IPI
    cysteine-type peptidase activity GO:0008234 TAS
    K63-linked deubiquitinase activity GO:0061578 IDA
    co-SMAD binding GO:0070410 IPI
    deubiquitinase activity GO:0101005 IDA
    deubiquitinase activity GO:0101005 ISS
    molecular sequestering activity GO:0140313 IDA
    K11-linked deubiquitinase activity GO:0180017 IDA
    K48-linked deubiquitinase activity GO:1990380 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 TAS
    neuron migration GO:0001764 IMP
    DNA alkylation repair GO:0006307 IDA
    chromosome segregation GO:0007059 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 IMP
    female gamete generation GO:0007292 TAS
    protein localization GO:0008104 TAS
    cell migration GO:0016477 IBA
    protein import into peroxisome matrix, receptor recycling GO:0016562 IDA
    protein ubiquitination GO:0016567 TAS
    protein deubiquitination GO:0016579 IDA
    protein deubiquitination GO:0016579 ISS
    protein deubiquitination GO:0016579 TAS
    BMP signaling pathway GO:0030509 IDA
    regulation of protein stability GO:0031647 IBA
    positive regulation of protein binding GO:0032092 IMP
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IMP
    monoubiquitinated protein deubiquitination GO:0035520 IDA
    regulation of circadian rhythm GO:0042752 IMP
    rhythmic process GO:0048511 IEA
    axon extension GO:0048675 IMP
    protein stabilization GO:0050821 IMP
    cell division GO:0051301 IEA
    cilium assembly GO:0060271 IDA
    cytosolic ciliogenesis GO:0061824 IDA
    protein K63-linked deubiquitination GO:0070536 IDA
    protein deubiquitination involved in ubiquitin-dependent protein catabolic process GO:0071947 IMP
    positive regulation of TORC2 signaling GO:1904515 IDA
    amyloid fibril formation GO:1990000 TAS
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cilium GO:0005929 IDA
    membrane GO:0016020 HDA
    growth cone GO:0030426 IDA
 Experiment description of studies that identified USP9X in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
11
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for USP9X
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDF1  
Affinity Capture-MS Homo sapiens
2 PRICKLE2  
Affinity Capture-Western Homo sapiens
3 PLCD4  
Affinity Capture-MS Homo sapiens
4 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
5 CPNE2 221184
Affinity Capture-MS Homo sapiens
6 PRICKLE1  
Affinity Capture-Western Homo sapiens
7 KPNA3 3839
Co-fractionation Homo sapiens
8 VARS 7407
Co-fractionation Homo sapiens
9 GEMIN4 50628
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 SHC1 6464
Affinity Capture-MS Homo sapiens
11 WWP1 11059
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 PRPF8 10594
Co-fractionation Homo sapiens
13 RIN3  
Affinity Capture-MS Homo sapiens
14 PSMA4 5685
Co-fractionation Homo sapiens
15 RIT1 6016
Negative Genetic Homo sapiens
16 ARL6IP5 10550
Co-fractionation Homo sapiens
17 RPA2 6118
Proximity Label-MS Homo sapiens
18 ZNHIT2 741
Affinity Capture-MS Homo sapiens
19 H2AFY 9555
Co-fractionation Homo sapiens
20 SMAD4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 TDRD3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
22 CCT4 10575
Co-fractionation Homo sapiens
23 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
24 FAM168A  
Affinity Capture-MS Homo sapiens
25 VHL  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
26 CAND1 55832
Affinity Capture-MS Homo sapiens
27 GJA1 2697
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
28 RPS6KB2  
Affinity Capture-MS Homo sapiens
29 PSMB8 5696
Co-fractionation Homo sapiens
30 Oxnad1  
Affinity Capture-MS Mus musculus
31 NUDCD2 134492
Co-fractionation Homo sapiens
32 DPPA4  
Affinity Capture-MS Homo sapiens
33 MAP3K5 4217
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Biochemical Activity Homo sapiens
34 RUVBL1 8607
Affinity Capture-MS Homo sapiens
35 PJA1  
Affinity Capture-MS Homo sapiens
36 MLLT4 4301
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 ZNF408  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 KRT8 3856
Proximity Label-MS Homo sapiens
39 MCM2 4171
Affinity Capture-MS Homo sapiens
40 NFKBIL1  
Affinity Capture-MS Homo sapiens
41 RPA3 6119
Proximity Label-MS Homo sapiens
42 SEC24C 9632
Co-fractionation Homo sapiens
43 NFE2L2 4780
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
44 SUPV3L1 6832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 SUV39H1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 POU5F1  
Affinity Capture-MS Homo sapiens
47 DDRGK1 65992
Affinity Capture-MS Homo sapiens
48 RECQL4  
Affinity Capture-MS Homo sapiens
49 HMGA1 3159
Affinity Capture-MS Homo sapiens
50 UBQLN1 29979
Co-fractionation Homo sapiens
51 CEP170 9859
Affinity Capture-MS Homo sapiens
52 PTPN14 5784
Affinity Capture-MS Homo sapiens
53 PCM1 5108
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
54 ZNF598 90850
Affinity Capture-Western Homo sapiens
55 MARK4  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
56 SEC23A 10484
Co-fractionation Homo sapiens
57 HUWE1 10075
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
58 SERBP1 26135
Affinity Capture-MS Homo sapiens
59 SOD1 6647
Affinity Capture-MS Homo sapiens
60 DVL2 1856
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
61 CLK3  
Affinity Capture-MS Homo sapiens
62 KIF14 9928
Affinity Capture-MS Homo sapiens
63 GEMIN2 8487
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
64 GTF2I 2969
Cross-Linking-MS (XL-MS) Homo sapiens
65 UNK  
Co-fractionation Homo sapiens
Affinity Capture-RNA Homo sapiens
66 ZYX 7791
Co-fractionation Homo sapiens
67 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 AMOTL2 51421
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
69 GPSM1 26086
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
70 IRS4 8471
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
71 LMNB1 4001
Proximity Label-MS Homo sapiens
72 TCP1 6950
Co-fractionation Homo sapiens
73 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
74 MIB1 57534
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
75 UBE4B 10277
Co-fractionation Homo sapiens
76 PSMB4 5692
Co-fractionation Homo sapiens
77 MARCH7  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
78 CRY1  
Affinity Capture-MS Homo sapiens
79 PARK2  
Affinity Capture-MS Homo sapiens
80 MAP1B 4131
Co-fractionation Homo sapiens
81 PEG10 23089
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 RNF115  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
83 MAGED1 9500
Affinity Capture-MS Homo sapiens
84 KPNA4 3840
Co-fractionation Homo sapiens
85 SNRNP200 23020
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
86 DDX20 11218
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
87 PTPN21 11099
Affinity Capture-MS Homo sapiens
88 CDH1 999
Co-fractionation Homo sapiens
89 CHMP4B 128866
Affinity Capture-MS Homo sapiens
90 YAP1 10413
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
91 GATA3  
Affinity Capture-MS Homo sapiens
92 ARRDC2  
Affinity Capture-MS Homo sapiens
93 NTRK1 4914
Affinity Capture-MS Homo sapiens
94 UBQLN2 29978
Co-fractionation Homo sapiens
95 SMAD3 4088
Affinity Capture-Western Homo sapiens
96 ALKBH3  
Affinity Capture-Western Homo sapiens
97 IQCB1  
Affinity Capture-MS Homo sapiens
98 MAGED4  
Affinity Capture-MS Homo sapiens
99 PYCRL 65263
Affinity Capture-MS Homo sapiens
100 SMAD2 4087
Affinity Capture-Western Homo sapiens
101 FBXL6  
Affinity Capture-MS Homo sapiens
102 MALT1 10892
Affinity Capture-Western Homo sapiens
103 CRY2  
Affinity Capture-MS Homo sapiens
104 CSNK1E 1454
Affinity Capture-MS Homo sapiens
105 ASB15 142685
Affinity Capture-MS Homo sapiens
106 AGPS 8540
Proximity Label-MS Homo sapiens
107 LATS2 26524
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
108 HDAC5 10014
Affinity Capture-MS Homo sapiens
109 Ksr1  
Affinity Capture-MS Mus musculus
110 SEC61B 10952
Proximity Label-MS Homo sapiens
111 NFX1  
Affinity Capture-MS Homo sapiens
112 TTLL12 23170
Co-fractionation Homo sapiens
113 EEF1D 1936
Co-fractionation Homo sapiens
114 IGBP1 3476
Co-fractionation Homo sapiens
115 CASP4  
Affinity Capture-MS Homo sapiens
116 C9orf72  
Affinity Capture-MS Homo sapiens
117 HADHA 3030
Co-fractionation Homo sapiens
118 UBE2H 7328
Affinity Capture-MS Homo sapiens
119 UFC1 51506
Co-fractionation Homo sapiens
120 DGKG  
Affinity Capture-MS Homo sapiens
121 AHCYL1 10768
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
122 AMBRA1  
Affinity Capture-MS Homo sapiens
123 XPO1 7514
Affinity Capture-MS Homo sapiens
124 NUBP2 10101
Co-fractionation Homo sapiens
125 ECT2 1894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 SRXN1 140809
Co-fractionation Homo sapiens
127 SIRT7  
Affinity Capture-MS Homo sapiens
128 UBXN7 26043
Co-fractionation Homo sapiens
129 SNRPB 6628
Affinity Capture-Western Homo sapiens
130 UBE2L3 7332
Co-fractionation Homo sapiens
131 CTNNB1 1499
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
132 PRKAA2 5563
Affinity Capture-Western Homo sapiens
133 GEMIN8 54960
Affinity Capture-Western Homo sapiens
134 MEX3A  
Affinity Capture-RNA Homo sapiens
135 CD274 29126
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
136 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 NR3C1 2908
Proximity Label-MS Homo sapiens
138 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
139 IKBKAP 8518
Affinity Capture-MS Homo sapiens
140 ATXN10 25814
Affinity Capture-MS Homo sapiens
141 NUAK1  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
142 ZFYVE19 84936
Co-fractionation Homo sapiens
143 PSD4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
144 EFTUD2 9343
Co-fractionation Homo sapiens
145 PTGES 9536
Affinity Capture-Western Homo sapiens
146 UBE2D2 7322
Co-fractionation Homo sapiens
147 DCAF6 55827
Co-fractionation Homo sapiens
148 MSR1  
Co-localization Homo sapiens
149 ABT1 29777
Affinity Capture-MS Homo sapiens
150 CNR2  
Affinity Capture-MS Homo sapiens
151 HECW2  
Affinity Capture-MS Homo sapiens
152 ATP2A1 487
Proximity Label-MS Homo sapiens
153 RASSF10  
Affinity Capture-MS Homo sapiens
154 MTOR 2475
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
155 PTPDC1  
Affinity Capture-MS Homo sapiens
156 PIK3R2 5296
Affinity Capture-MS Homo sapiens
157 RICTOR 253260
Affinity Capture-Western Homo sapiens
158 UBL7  
Co-fractionation Homo sapiens
159 PCGF1 84759
Affinity Capture-MS Homo sapiens
160 AMOT 154796
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
161 DCX  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
162 Yap1  
Reconstituted Complex Mus musculus
163 Nedd1  
Affinity Capture-MS Mus musculus
164 KIAA0368 23392
Affinity Capture-MS Homo sapiens
165 LDLR 3949
Negative Genetic Homo sapiens
166 NANOG  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
167 PEX3 8504
Proximity Label-MS Homo sapiens
168 AKAP1 8165
Proximity Label-MS Homo sapiens
169 UBXN1 51035
Co-fractionation Homo sapiens
170 MLLT4  
Reconstituted Complex Bos taurus
171 ZNF263  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
172 CCDC8  
Affinity Capture-MS Homo sapiens
173 BIRC5  
Affinity Capture-Western Homo sapiens
174 TCF25 22980
Affinity Capture-MS Homo sapiens
175 UBE2D1 7321
Co-fractionation Homo sapiens
Co-purification Homo sapiens
176 HIF1A 3091
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
177 RBM14 10432
Affinity Capture-MS Homo sapiens
178 GRK5 2869
Affinity Capture-MS Homo sapiens
179 SNCA 6622
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
180 PEF1 553115
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
181 CEP290  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
182 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
183 FASN 2194
Negative Genetic Homo sapiens
184 TERF2  
Affinity Capture-MS Homo sapiens
185 SIRT6  
Affinity Capture-MS Homo sapiens
186 SMN1 6606
Affinity Capture-MS Homo sapiens
187 SNAI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
188 EMD 2010
Proximity Label-MS Homo sapiens
189 TP53 7157
Affinity Capture-MS Homo sapiens
190 USP34 9736
Co-fractionation Homo sapiens
191 Atp7a  
Affinity Capture-MS Mus musculus
192 NEK6 10783
Affinity Capture-MS Homo sapiens
193 Msn 17698
Affinity Capture-MS Mus musculus
194 ZNF746  
Affinity Capture-MS Homo sapiens
195 MCL1 4170
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
196 WDR54  
Affinity Capture-MS Homo sapiens
197 UBA52 7311
Co-fractionation Homo sapiens
198 SMAD1 4086
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
199 RPTOR 57521
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
200 SMURF1 57154
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
201 UBE2C 11065
Co-fractionation Homo sapiens
202 ITCH 83737
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
203 SMN2 6607
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
204 ZNF764 92595
Affinity Capture-MS Homo sapiens
205 BCL10  
Affinity Capture-Western Homo sapiens
206 GNL1 2794
Co-fractionation Homo sapiens
207 UCHL3 7347
Co-fractionation Homo sapiens
208 NIFK 84365
Proximity Label-MS Homo sapiens
209 ATP6V1F 9296
Co-fractionation Homo sapiens
210 PBX1 5087
Affinity Capture-Western Homo sapiens
211 WWP2 11060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
212 KIAA1429 25962
Affinity Capture-MS Homo sapiens
213 TERF1 7013
Affinity Capture-MS Homo sapiens
214 OTUD4  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
215 HEXIM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
216 UBR4 23352
Co-fractionation Homo sapiens
217 EIF4B 1975
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
218 C16orf72 29035
Affinity Capture-MS Homo sapiens
219 SSSCA1 10534
Co-fractionation Homo sapiens
220 UBE2B 7320
Co-fractionation Homo sapiens
221 GEMIN5 25929
Affinity Capture-MS Homo sapiens
222 ERG  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
223 ALDH1A3 220
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
224 PEX5 5830
Biochemical Activity Homo sapiens
225 ANK3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
226 MZF1  
Affinity Capture-MS Homo sapiens
227 RC3H1 149041
Affinity Capture-MS Homo sapiens
228 DDX3X 1654
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
229 MTMR1 8776
Affinity Capture-MS Homo sapiens
230 KLHL12  
Affinity Capture-MS Homo sapiens
231 PRMT1 3276
Affinity Capture-MS Homo sapiens
232 UBE2V2 7336
Co-fractionation Homo sapiens
233 UFM1 51569
Co-fractionation Homo sapiens
234 EP300 2033
Affinity Capture-MS Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here