Gene description for FLOT1
Gene name flotillin 1
Gene symbol FLOT1
Other names/aliases -
Species Homo sapiens
 Database cross references - FLOT1
ExoCarta ExoCarta_10211
Vesiclepedia VP_10211
Entrez Gene 10211
HGNC 3757
MIM 606998
UniProt O75955  
 FLOT1 identified in exosomes derived from the following tissue/cell type
Aqueous humor 21362503    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34112803    
Breast cancer cells 34112803    
Breast cancer cells 35939247    
Breast cancer cells 35939247    
Breast cancer cells 35939247    
Breast cancer cells 35939247    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 34887515    
Breast cancer cells 34887515    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colon cancer cells 34887515    
Colon cancer cells 34887515    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Colorectal cancer cells 34887515    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Endothelial cells 26027894    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lung cancer cells 34887515    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 26176991    
Melanoma cells 34789738    
Melanoma cells 34789738    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34887515    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Plasma 34112803    
Plasma 34887515    
Plasma 34887515    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Prostate cancer cells 25519911    
Prostate cancer cells 25519911    
Prostate cancer cells 25519911    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Retinal endothelial cells 34887515    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 33432243    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Tabecular meshwork cells 21362503    
Thymus 23844026    
Urine 15326289    
Urine 19056867    
Urine 21362503    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
Urine 33432243    
 Gene ontology annotations for FLOT1
Molecular Function
    protease binding GO:0002020 IBA
    protease binding GO:0002020 IPI
    protein binding GO:0005515 IPI
    ionotropic glutamate receptor binding GO:0035255 IEA
Biological Process
    positive regulation of cytokine production GO:0001819 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    regulation of receptor internalization GO:0002090 IBA
    regulation of receptor internalization GO:0002090 IMP
    axonogenesis GO:0007409 IEA
    extracellular matrix disassembly GO:0022617 IMP
    positive regulation of protein binding GO:0032092 IMP
    positive regulation of synaptic transmission, dopaminergic GO:0032226 ISS
    positive regulation of interferon-beta production GO:0032728 IMP
    dsRNA transport GO:0033227 IMP
    positive regulation of heterotypic cell-cell adhesion GO:0034116 IMP
    positive regulation of toll-like receptor 3 signaling pathway GO:0034141 IMP
    regulation of toll-like receptor 4 signaling pathway GO:0034143 IMP
    response to endoplasmic reticulum stress GO:0034976 IMP
    regulation of Rho protein signal transduction GO:0035023 IEA
    intracellular signal transduction GO:0035556 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    plasma membrane raft assembly GO:0044854 IMP
    plasma membrane raft organization GO:0044857 IMP
    positive regulation of endocytosis GO:0045807 IBA
    positive regulation of endocytosis GO:0045807 IMP
    positive regulation of skeletal muscle tissue development GO:0048643 IMP
    protein stabilization GO:0050821 IMP
    regulation of neurotransmitter uptake GO:0051580 IDA
    regulation of neurotransmitter uptake GO:0051580 IMP
    protein kinase C signaling GO:0070528 IBA
    cellular response to exogenous dsRNA GO:0071360 IMP
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IMP
    positive regulation of myoblast fusion GO:1901741 IMP
    positive regulation of cell junction assembly GO:1901890 IBA
    positive regulation of cell junction assembly GO:1901890 IMP
    positive regulation of cell-cell adhesion mediated by cadherin GO:2000049 IBA
    positive regulation of cell-cell adhesion mediated by cadherin GO:2000049 IMP
Subcellular Localization
    uropod GO:0001931 IDA
    lysosomal membrane GO:0005765 HDA
    endosome GO:0005768 IDA
    early endosome GO:0005769 IDA
    microtubule organizing center GO:0005815 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    caveola GO:0005901 TAS
    cell-cell junction GO:0005911 IDA
    adherens junction GO:0005912 IDA
    focal adhesion GO:0005925 HDA
    COP9 signalosome GO:0008180 IDA
    external side of plasma membrane GO:0009897 IEA
    membrane GO:0016020 HDA
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IDA
    flotillin complex GO:0016600 IBA
    flotillin complex GO:0016600 IDA
    lamellipodium GO:0030027 IDA
    cortical actin cytoskeleton GO:0030864 IDA
    cytoplasmic vesicle GO:0031410 IBA
    cytoplasmic vesicle GO:0031410 IDA
    centriolar satellite GO:0034451 IDA
    sarcolemma GO:0042383 IEA
    melanosome GO:0042470 IEA
    cell-cell contact zone GO:0044291 IDA
    membrane raft GO:0045121 IDA
    presynaptic active zone GO:0048786 IEA
    extracellular exosome GO:0070062 HDA
    dopaminergic synapse GO:0098691 IDA
    dopaminergic synapse GO:0098691 IMP
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified FLOT1 in exosomes
1
Experiment ID 120
MISEV standards
Biophysical techniques
GAPDH|CD9|CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21362503    
Organism Homo sapiens
Experiment description Protein profile of exosomes from trabecular meshwork cells.
Authors "Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL."
Journal name JOP
Publication year 2011
Sample Aqueous humor
Sample name Aqueous humor - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 79
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 80
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 81
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 76
MISEV standards
EM
Biophysical techniques
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
6
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 412
MISEV standards
EM
Biophysical techniques
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
CANX|ACTB
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
11
Experiment ID 414
MISEV standards
EM
Biophysical techniques
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 426
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 426
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 426
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 427
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 429
MISEV standards
Biophysical techniques
FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
17
Experiment ID 430
MISEV standards
Biophysical techniques
FLOT1|SDCBP
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 431
MISEV standards
Biophysical techniques
FLOT1|SDCBP
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 4
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
19
Experiment ID 432
MISEV standards
Biophysical techniques
FLOT1|SDCBP
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 433
MISEV standards
Biophysical techniques
FLOT1|SDCBP
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 522
MISEV standards
EM
Biophysical techniques
FLOT1|CD9
Enriched markers
GOLGA2
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Homo sapiens
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 523
MISEV standards
EM
Biophysical techniques
FLOT1|CD9
Enriched markers
GOLGA2
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Homo sapiens
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
23
Experiment ID 679
MISEV standards
EM
Biophysical techniques
FLOT1|TSG101
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 35939247    
Organism Homo sapiens
Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors "Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name Clin Exp Metastasis
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-231T
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
24
Experiment ID 680
MISEV standards
EM
Biophysical techniques
FLOT1|TSG101
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 35939247    
Organism Homo sapiens
Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors "Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name Clin Exp Metastasis
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-231T
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 681
MISEV standards
EM
Biophysical techniques
FLOT1|TSG101
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 35939247    
Organism Homo sapiens
Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors "Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name Clin Exp Metastasis
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-435
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 682
MISEV standards
EM
Biophysical techniques
FLOT1|TSG101
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 35939247    
Organism Homo sapiens
Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors "Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name Clin Exp Metastasis
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-435
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 924
MISEV standards
EM
Biophysical techniques
CD9|SDCBP|CD81|RAB35|LAMP2|FLOT1|TSG101|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 926
MISEV standards
Biophysical techniques
CD9|FLOT1|TSG101|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 930
MISEV standards
EM
Biophysical techniques
CD9|FLOT1|TSG101|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
30
Experiment ID 932
MISEV standards
Biophysical techniques
CD9|FLOT1|CD63
Enriched markers
Negative markers
NTA