|
 Gene ontology annotations for FLOT1 |
|
|
| Experiment description of studies that identified FLOT1 in sEVs |
| 1 |
| Experiment ID |
120 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
GAPDH|CD9|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
21362503
|
| Organism |
Homo sapiens |
| Experiment description |
Protein profile of exosomes from trabecular meshwork cells. |
| Authors |
"Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL." |
| Journal name |
JOP
|
| Publication year |
2011 |
| Sample |
Aqueous humor |
| Sample name |
Aqueous humor - Normal |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 2 |
| Experiment ID |
79 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|MHCII
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20458337
|
| Organism |
Homo sapiens |
| Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 |
| Authors |
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." |
| Journal name |
ICB
|
| Publication year |
2010 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [FT-ICR]
Western blotting |
|
|
| 3 |
| Experiment ID |
80 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|MHCII
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20458337
|
| Organism |
Homo sapiens |
| Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 |
| Authors |
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." |
| Journal name |
ICB
|
| Publication year |
2010 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [FT-ICR]
Western blotting |
|
|
| 4 |
| Experiment ID |
81 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|MHCII
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20458337
|
| Organism |
Homo sapiens |
| Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 |
| Authors |
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." |
| Journal name |
ICB
|
| Publication year |
2010 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [FT-ICR]
Western blotting |
|
|
| 5 |
| Experiment ID |
76 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20224111
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomics analysis of bladder cancer exosomes. |
| Authors |
"Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A." |
| Journal name |
MCP
|
| Publication year |
2010 |
| Sample |
Bladder cancer cells |
| Sample name |
HT1376 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS |
|
|
| 6 |
| Experiment ID |
489 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 7 |
| Experiment ID |
490 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 8 |
| Experiment ID |
491 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 8 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 9 |
| Experiment ID |
492 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 9 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 10 |
| Experiment ID |
412 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX|ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 11 |
| Experiment ID |
414 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 12 |
| Experiment ID |
426 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 13 |
| Experiment ID |
426 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 14 |
| Experiment ID |
426 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 15 |
| Experiment ID |
427 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 16 |
| Experiment ID |
429 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 17 |
| Experiment ID |
430 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 18 |
| Experiment ID |
431 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 4 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 19 |
| Experiment ID |
432 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 20 |
| Experiment ID |
433 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 21 |
| Experiment ID |
522 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|CD9
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34112803
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 22 |
| Experiment ID |
523 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|CD9
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34112803
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 23 |
| Experiment ID |
679 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35939247
|
| Organism |
Homo sapiens |
| Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
| Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
| Journal name |
Clin Exp Metastasis
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 24 |
| Experiment ID |
680 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35939247
|
| Organism |
Homo sapiens |
| Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
| Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
| Journal name |
Clin Exp Metastasis
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 25 |
| Experiment ID |
681 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35939247
|
| Organism |
Homo sapiens |
| Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
| Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
| Journal name |
Clin Exp Metastasis
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-435 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 26 |
| Experiment ID |
682 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35939247
|
| Organism |
Homo sapiens |
| Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
| Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
| Journal name |
Clin Exp Metastasis
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-435 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 27 |
| Experiment ID |
924 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|SDCBP|CD81|RAB35|LAMP2|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 28 |
| Experiment ID |
926 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 29 |
| Experiment ID |
930 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 30 |
| Experiment ID |
932 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|FLOT1|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 31 |
| Experiment ID |
933 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|FLOT1|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 32 |
| Experiment ID |
1181 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 33 |
| Experiment ID |
1182 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 34 |
| Experiment ID |
1184 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 35 |
| Experiment ID |
1185 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 36 |
| Experiment ID |
1186 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 37 |
| Experiment ID |
1187 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 8 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 38 |
| Experiment ID |
1188 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 9 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 39 |
| Experiment ID |
1189 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 1 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
1.05 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 40 |
| Experiment ID |
1190 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 3 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 41 |
| Experiment ID |
1191 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 4 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 42 |
| Experiment ID |
1192 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 43 |
| Experiment ID |
1193 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 44 |
| Experiment ID |
1194 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 45 |
| Experiment ID |
1195 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 8 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 46 |
| Experiment ID |
1196 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 9 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 47 |
| Experiment ID |
1197 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 48 |
| Experiment ID |
1198 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 49 |
| Experiment ID |
1206 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry
ELISA |
|
|
| 50 |
| Experiment ID |
1210 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
LM2-4175 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 51 |
| Experiment ID |
494 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Osteoarthritic cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 52 |
| Experiment ID |
496 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Healthy cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 53 |
| Experiment ID |
497 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 54 |
| Experiment ID |
498 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 55 |
| Experiment ID |
1213 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|CD9|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colon cancer cells |
| Sample name |
DiFi-derived EGFR/CD81-positive sEVs - dim (FAVS) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 56 |
| Experiment ID |
1214 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
EPCAM|FLOT1|CD9|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colon cancer cells |
| Sample name |
DiFi-derived EGFR/CD81-positive sEVs - bright (FAVS) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 57 |
| Experiment ID |
20 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP90|CD63|CD81|LAMP1
|
Enriched markers |
|
✔
GOLGA2|cytochrome c
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
17956143
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of microvesicles derived from human colorectal cancer cells. |
| Authors |
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS" |
| Journal name |
JPR
|
| Publication year |
2007 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Diafiltration |
| Flotation density |
1.16 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LTQ]
Western blotting |
|
|
| 58 |
| Experiment ID |
21 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
Alix|TSG101|HSP70|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
19837982
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. |
| Authors |
"Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" |
| Journal name |
MCP
|
| Publication year |
2009 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1215 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.12 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [Orbitrap]
Western blotting |
|
|
| 59 |
| Experiment ID |
207 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DKO-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 60 |
| Experiment ID |
208 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
Dks-8 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 61 |
| Experiment ID |
209 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DLD-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 62 |
| Experiment ID |
282 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 63 |
| Experiment ID |
283 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 64 |
| Experiment ID |
285 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 65 |
| Experiment ID |
286 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 66 |
| Experiment ID |
1202 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|CD9|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1215 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 67 |
| Experiment ID |
1203 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
DiFi |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA sequencing |
|
|
| 68 |
| Experiment ID |
1203 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
DiFi |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA sequencing |
|
|
| 69 |
| Experiment ID |
1207 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|CD9|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCA-7 SC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 70 |
| Experiment ID |
1340 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63|FLOT1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36068649
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway |
| Authors |
"Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 71 |
| Experiment ID |
1341 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63|FLOT1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36068649
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway |
| Authors |
"Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 72 |
| Experiment ID |
1361 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD63|FLOT1|LAMP1|CD24|EPCAM|Alix
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
MORPH
ELISA |
|
|
| 73 |
| Experiment ID |
407 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 74 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 75 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 76 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 77 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 78 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 79 |
| Experiment ID |
421 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 80 |
| Experiment ID |
422 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 81 |
| Experiment ID |
423 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 82 |
| Experiment ID |
424 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 4 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 83 |
| Experiment ID |
425 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 84 |
| Experiment ID |
1275 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 85 |
| Experiment ID |
1280 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.151 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 86 |
| Experiment ID |
226 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
GAPDH|CD9|FLOT1
|
Enriched markers |
|
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
26027894
|
| Organism |
Homo sapiens |
| Experiment description |
"Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" |
| Authors |
"Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" |
| Journal name |
Journal of Extracellular Vesicles
|
| Publication year |
2015 |
| Sample |
Endothelial cells |
| Sample name |
HMEC-1 |
| Isolation/purification methods |
Differential ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA |
| Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting |
|
|
| 87 |
| Experiment ID |
1348 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
FLOT1|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36266345
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression |
| Authors |
"Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J." |
| Journal name |
Nat Commun
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
MRC-5 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 88 |
| Experiment ID |
1349 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
FLOT1|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36266345
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression |
| Authors |
"Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J." |
| Journal name |
Nat Commun
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
MRC-5 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 89 |
| Experiment ID |
1350 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
FLOT1|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36266345
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression |
| Authors |
"Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J." |
| Journal name |
Nat Commun
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 90 |
| Experiment ID |
1351 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
FLOT1|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36266345
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression |
| Authors |
"Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J." |
| Journal name |
Nat Commun
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 91 |
| Experiment ID |
405 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Foreskin fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 92 |
| Experiment ID |
363 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 93 |
| Experiment ID |
364 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 94 |
| Experiment ID |
365 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 95 |
| Experiment ID |
1201 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|CD9|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
Calu-3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 96 |
| Experiment ID |
1324 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
GAPDH|CD63|LAMP1|FLOT1|TSG101|Alix
|
Enriched markers |
|
✔
GAPDH|HSP90AA1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33686255
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicle drug occupancy enables real-time monitoring of targeted cancer therapy. |
| Authors |
"Pan S, Zhang Y, Natalia A, Lim CZJ, Ho NRY, Chowbay B, Loh TP, Tam JKC, Shao H." |
| Journal name |
Nat Nanotechnol
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H3255 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 97 |
| Experiment ID |
417 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX|ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Lymphoma cells |
| Sample name |
Raji |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 98 |
| Experiment ID |
411 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Mammary cancer-associated fibroblasts |
| Sample name |
mCAF |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 99 |
| Experiment ID |
254 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
MNT-1 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 100 |
| Experiment ID |
255 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
G1 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 101 |
| Experiment ID |
256 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
501mel |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 102 |
| Experiment ID |
257 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
Daju |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 103 |
| Experiment ID |
258 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
SKMEL28 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 104 |
| Experiment ID |
259 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
A375M |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 105 |
| Experiment ID |
260 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
1205Lu |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 106 |
| Experiment ID |
265 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
26176991
|
| Organism |
Homo sapiens |
| Experiment description |
Small RNA deep sequencing discriminates subsets of extracellular vesicles released by melanoma cells - evidence of unique microRNA cargos |
| Authors |
"Lunavat TR, Cheng L, Kim DK, Bhadury J, Jang SC, Lasser C, Sharples RA, Lopez MD, Nilsson J, Gho YS, Hill AF, Lotvall J." |
| Journal name |
RNA Biology
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
MML-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA
snRNA
snoRNA
mtRNA
Ro associated Y-RNA
MiscRNA |
| Methods used in the study |
Western blotting
RNA deep sequencing |
|
|
| 107 |
| Experiment ID |
1091 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34789738
|
| Organism |
Homo sapiens |
| Experiment description |
P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells |
| Authors |
"Pegoraro A, De Marchi E, Ferracin M, Orioli E, Zanoni M, Bassi C, Tesei A, Capece M, Dika E, Negrini M, Di Virgilio F, Adinolfi E." |
| Journal name |
Cell Death Dis
|
| Publication year |
2021 |
| Sample |
Melanoma cells |
| Sample name |
SK-MEL-28 - 100k pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Small RNA sequencing
RT-PCR |
|
|
| 108 |
| Experiment ID |
1092 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34789738
|
| Organism |
Homo sapiens |
| Experiment description |
P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells |
| Authors |
"Pegoraro A, De Marchi E, Ferracin M, Orioli E, Zanoni M, Bassi C, Tesei A, Capece M, Dika E, Negrini M, Di Virgilio F, Adinolfi E." |
| Journal name |
Cell Death Dis
|
| Publication year |
2021 |
| Sample |
Melanoma cells |
| Sample name |
Ma-Mel-19 - 100k pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Small RNA sequencing
RT-PCR |
|
|
| 109 |
| Experiment ID |
126 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
| PubMed ID |
Unpublished / Not applicable
|
| Organism |
Homo sapiens |
| Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
| Authors |
"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" |
| Journal name |
Mesenchymal Stem Cell Therapy
|
| Publication year |
2011 |
| Sample |
Mesenchymal stem cells |
| Sample name |
huES9.E1 |
| Isolation/purification methods |
HPLC |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Antobody array
Mass spectrometry |
|
|
| 110 |
| Experiment ID |
488 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Mesenchymal stem cells |
| Sample name |
UCMSC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 111 |
| Experiment ID |
418 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Monocytic leukemia cells |
| Sample name |
THP-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 112 |
| Experiment ID |
224 |
| MISEV standards |
|
✔
EM|AFM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25944692
|
| Organism |
Homo sapiens |
| Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
| Authors |
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Neuroblastoma cells |
| Sample name |
SH-SY5Y |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
OptiPrep density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 113 |
| Experiment ID |
1339 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63|FLOT1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36068649
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway |
| Authors |
"Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Normal colon fibroblasts |
| Sample name |
CCD18Co |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 114 |
| Experiment ID |
413 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Normal mammary epithelial cells |
| Sample name |
MCF10A |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 115 |
| Experiment ID |
211 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
|
✔
cytochrome c|GOLGA2
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
IGROV1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 116 |
| Experiment ID |
212 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
|
✔
Cytochrome C|GOLGA2
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
OVCAR-3 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 117 |
| Experiment ID |
406 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
BxPC3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 118 |
| Experiment ID |
415 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 119 |
| Experiment ID |
434 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 120 |
| Experiment ID |
434 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 121 |
| Experiment ID |
435 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 122 |
| Experiment ID |
437 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Fraction 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 123 |
| Experiment ID |
438 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Fraction 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 124 |
| Experiment ID |
439 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Fraction 4 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 125 |
| Experiment ID |
440 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 126 |
| Experiment ID |
1205 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 127 |
| Experiment ID |
408 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX|ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic duct epithalial cells |
| Sample name |
HPDE |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 128 |
| Experiment ID |
409 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic duct epithalial cells |
| Sample name |
HPNE |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 129 |
| Experiment ID |
524 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|CD9
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34112803
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Plasma |
| Sample name |
Pooled plasma from healthy subjects and breast cancer patients |
| Isolation/purification methods |
Size exclusion chromatography
Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 130 |
| Experiment ID |
1204 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Plasma |
| Sample name |
Colorectal cancer patients |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
ELISA |
|
|
| 131 |
| Experiment ID |
1209 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Plasma |
| Sample name |
Control individuals |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 132 |
| Experiment ID |
231 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
Alix|CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25332113
|
| Organism |
Homo sapiens |
| Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
| Authors |
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." |
| Journal name |
Transfusion
|
| Publication year |
2015 |
| Sample |
Platelets |
| Sample name |
PL-Exs - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient |
| Flotation density |
1.12-1.15 g/mL
|
| Molecules identified in the study |
Protein
Lipids |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 133 |
| Experiment ID |
232 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25332113
|
| Organism |
Homo sapiens |
| Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
| Authors |
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." |
| Journal name |
Transfusion
|
| Publication year |
2015 |
| Sample |
Platelets |
| Sample name |
PL-Exs - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient |
| Flotation density |
1.12-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 134 |
| Experiment ID |
233 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25332113
|
| Organism |
Homo sapiens |
| Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
| Authors |
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." |
| Journal name |
Transfusion
|
| Publication year |
2015 |
| Sample |
Platelets |
| Sample name |
PL-Exs - Rep 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient |
| Flotation density |
1.12-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 135 |
| Experiment ID |
416 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pluripotent stem cells |
| Sample name |
PSC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 136 |
| Experiment ID |
245 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|CD9|CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25519911
|
| Organism |
Homo sapiens |
| Experiment description |
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells |
| Authors |
"Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A." |
| Journal name |
J Biol Chem
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
PC-3 - Ethanol treated (control) |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Lipids
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 137 |
| Experiment ID |
246 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|CD9|CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25519911
|
| Organism |
Homo sapiens |
| Experiment description |
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells |
| Authors |
"Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A." |
| Journal name |
J Biol Chem
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
PC-3 - sn-1-O-hexadecylglycerol treated |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Lipids |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 138 |
| Experiment ID |
247 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|Alix|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25519911
|
| Organism |
Homo sapiens |
| Experiment description |
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells |
| Authors |
"Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A." |
| Journal name |
J Biol Chem
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
PC-3 - DL- palmitin treated |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Lipids |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 139 |
| Experiment ID |
275 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
|
Enriched markers |
|
✔
AIF
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25844599
|
| Organism |
Homo sapiens |
| Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
| Authors |
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Docetaxel sensitive |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.12-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry/Flow cytometry/Western blotting |
|
|
| 140 |
| Experiment ID |
274 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
|
Enriched markers |
|
✔
AIF
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25844599
|
| Organism |
Homo sapiens |
| Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
| Authors |
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Docetaxel resistant |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Flow cytometry
Western blotting |
|
|
| 141 |
| Experiment ID |
1208 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Retinal endothelial cells |
| Sample name |
HREC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 142 |
| Experiment ID |
834 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 143 |
| Experiment ID |
834 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 144 |
| Experiment ID |
835 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 145 |
| Experiment ID |
835 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 146 |
| Experiment ID |
836 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
LAMP2|CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 147 |
| Experiment ID |
837 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
LAMP2|CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 148 |
| Experiment ID |
838 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 149 |
| Experiment ID |
839 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
LAMP2|CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 150 |
| Experiment ID |
840 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 151 |
| Experiment ID |
841 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 152 |
| Experiment ID |
842 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 153 |
| Experiment ID |
843 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 154 |
| Experiment ID |
844 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
LAMP2|CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 155 |
| Experiment ID |
845 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 156 |
| Experiment ID |
848 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 157 |
| Experiment ID |
849 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 158 |
| Experiment ID |
850 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 159 |
| Experiment ID |
851 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 160 |
| Experiment ID |
852 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 161 |
| Experiment ID |
853 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 162 |
| Experiment ID |
854 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 163 |
| Experiment ID |
855 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 164 |
| Experiment ID |
856 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 165 |
| Experiment ID |
857 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 166 |
| Experiment ID |
858 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 167 |
| Experiment ID |
859 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 168 |
| Experiment ID |
860 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 169 |
| Experiment ID |
863 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 170 |
| Experiment ID |
864 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 171 |
| Experiment ID |
865 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 172 |
| Experiment ID |
866 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 173 |
| Experiment ID |
867 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 174 |
| Experiment ID |
191 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
Alix|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20124223
|
| Organism |
Homo sapiens |
| Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
| Authors |
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2010 |
| Sample |
Squamous carcinoma cells |
| Sample name |
Squamous carcinoma cell (A431) |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 175 |
| Experiment ID |
410 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
T lymphocytes |
| Sample name |
Jurkat |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 176 |
| Experiment ID |
118 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|GAPDH|CD9|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
21362503
|
| Organism |
Homo sapiens |
| Experiment description |
Protein profile of exosomes from trabecular meshwork cells. |
| Authors |
"Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL." |
| Journal name |
JOP
|
| Publication year |
2011 |
| Sample |
Tabecular meshwork cells |
| Sample name |
Tabecular meshwork cells |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.07-1.2 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LTQ]
Western blotting |
|
|
| 177 |
| Experiment ID |
217 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD81|CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23844026
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of human thymic exosomes. |
| Authors |
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." |
| Journal name |
PLoS One
|
| Publication year |
2013 |
| Sample |
Thymus |
| Sample name |
Normal-Thymus |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 178 |
| Experiment ID |
13 |
| MISEV standards |
|
✔
IEM
|
Biophysical techniques |
|
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
15326289
|
| Organism |
Homo sapiens |
| Experiment description |
Identification and proteomic profiling of exosomes in human urine. |
| Authors |
"Pisitkun T, Shen RF, Knepper MA" |
| Journal name |
PNAS
|
| Publication year |
2004 |
| Sample |
Urine |
| Sample name |
Urine - Normal |
| Isolation/purification methods |
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LCQ DECA XP]
Western blotting |
|
|
| 179 |
| Experiment ID |
63 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
AQP2
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
19056867
|
| Organism |
Homo sapiens |
| Experiment description |
Large-scale proteomics and phosphoproteomics of urinary exosomes. |
| Authors |
"Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" |
| Journal name |
JASN
|
| Publication year |
2009 |
| Sample |
Urine |
| Sample name |
Urine - Normal |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LTQ]
Western blotting |
|
|
| 180 |
| Experiment ID |
119 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
21362503
|
| Organism |
Homo sapiens |
| Experiment description |
Protein profile of exosomes from trabecular meshwork cells. |
| Authors |
"Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL." |
| Journal name |
JOP
|
| Publication year |
2011 |
| Sample |
Urine |
| Sample name |
Urine - Normal |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LTQ]
Western blotting |
|
|
| 181 |
| Experiment ID |
193 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
PHB
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21595033
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. |
| Authors |
"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." |
| Journal name |
Proteomics
|
| Publication year |
2011 |
| Sample |
Urine |
| Sample name |
Urine - Normal |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 182 |
| Experiment ID |
194 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21595033
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. |
| Authors |
"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." |
| Journal name |
Proteomics
|
| Publication year |
2011 |
| Sample |
Urine |
| Sample name |
Urine - Patients of basement membrane nephropathy |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 183 |
| Experiment ID |
195 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21595033
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. |
| Authors |
"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." |
| Journal name |
Proteomics
|
| Publication year |
2011 |
| Sample |
Urine |
| Sample name |
Urine - Patients of early IgA nephropathy |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 184 |
| Experiment ID |
196 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|TSG101|HSP70|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
22418980
|
| Organism |
Homo sapiens |
| Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
| Authors |
"Raj DA, Fiume I, Capasso G, Pocsfalvi G." |
| Journal name |
Kidney Int
|
| Publication year |
2012 |
| Sample |
Urine |
| Sample name |
Urine - Normal high density |
| Isolation/purification methods |
Differential centrifugation
Sucrose cushion |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 185 |
| Experiment ID |
197 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|TSG101|HSP70|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
22418980
|
| Organism |
Homo sapiens |
| Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
| Authors |
"Raj DA, Fiume I, Capasso G, Pocsfalvi G." |
| Journal name |
Kidney Int
|
| Publication year |
2012 |
| Sample |
Urine |
| Sample name |
Urine - Normal low density |
| Isolation/purification methods |
Differential centrifugation
Sucrose cushion |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| Protein-protein interactions for FLOT1 |
| |
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
| 1 |
ISG15 |
9636 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 2 |
WDR6 |
11180 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 3 |
UTRN |
7402 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 4 |
RASSF8 |
11228 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 5 |
NPLOC4 |
55666 |
| Co-fractionation |
|
Homo sapiens |
|
| 6 |
ETV6 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 7 |
STAT5B |
6777 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 8 |
LDHB |
3945 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 9 |
LLGL1 |
3996 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 10 |
PKP2 |
5318 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 11 |
JPH1 |
56704 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 12 |
ABCA1 |
19 |
| Affinity Capture-Western |
|
Homo sapiens |
|
| 13 |
KIF20A |
10112 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 14 |
C1orf226 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 15 |
UBC |
7316 |
| Affinity Capture-MS |
|
Homo sapiens |
| Reconstituted Complex |
|
Homo sapiens |
|
| 16 |
VHL |
|
| Two-hybrid |
|
Homo sapiens |
|
| 17 |
SEPT2 |
4735 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 18 |
ELF4 |
2000 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 19 |
CCDC90B |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 20 |
APEX1 |
328 |
| Affinity Capture-RNA |
|
Homo sapiens |
|
| 21 |
SEPT9 |
10801 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 22 |
PTRH2 |
51651 |
| Co-fractionation |
|
Homo sapiens |
|
| 23 |
KIF23 |
9493 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 24 |
PLCH1 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 25 |
UNC5B |
219699 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 26 |
SEL1L |
6400 |
| Co-fractionation |
|
Homo sapiens |
|
| 27 |
CALD1 |
800 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 28 |
SLC9A9 |
285195 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 29 |
NFATC3 |
4775 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 30 |
MARCH5 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 31 |
RASAL2 |
9462 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 32 |
BOD1L1 |
259282 |
| Cross-Linking-MS (XL-MS) |
|
Homo sapiens |
|
| 33 |
Flot2 |
14252 |
| Affinity Capture-MS |
|
Mus musculus |
|
| 34 |
SEPT7 |
989 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 35 |
LMAN2 |
10960 |
| Co-fractionation |
|
Homo sapiens |
|
| 36 |
MECP2 |
4204 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 37 |
Vps28 |
66914 |
| Affinity Capture-MS |
|
Mus musculus |
|
| 38 |
SLC12A7 |
10723 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 39 |
ZFYVE9 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 40 |
ARHGEF4 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 41 |
EXOC3 |
11336 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 42 |
SORBS1 |
10580 |
| Two-hybrid |
|
Homo sapiens |
| Affinity Capture-Western |
|
Homo sapiens |
| Affinity Capture-Western |
|
Homo sapiens |
| Reconstituted Complex |
|
Homo sapiens |
| Reconstituted Complex |
|
Homo sapiens |
| Affinity Capture-Western |
|
Homo sapiens |
|
| 43 |
NOTCH2 |
4853 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 44 |
EMC1 |
23065 |
| Co-fractionation |
|
Homo sapiens |
|
| 45 |
AP3M1 |
26985 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 46 |
DNAL4 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 47 |
VAV1 |
7409 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 48 |
SORBS2 |
|
| Affinity Capture-Western |
|
Homo sapiens |
|
| 49 |
ILVBL |
10994 |
| Co-fractionation |
|
Homo sapiens |
|
| 50 |
MCAM |
4162 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 51 |
EFR3B |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 52 |
CERS2 |
29956 |
| Co-fractionation |
|
Homo sapiens |
|
| 53 |
CDH1 |
999 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 54 |
YAP1 |
10413 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 55 |
MYH9 |
4627 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 56 |
SYNJ2BP |
55333 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 57 |
DTNB |
1838 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 58 |
GORASP2 |
26003 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 59 |
Coro1c |
23790 |
| Affinity Capture-MS |
|
Mus musculus |
|
| 60 |
MPP7 |
143098 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 61 |
FLOT2 |
2319 |
| Co-fractionation |
|
Homo sapiens |
| Proximity Label-MS |
|
Homo sapiens |
| Cross-Linking-MS (XL-MS) |
|
Homo sapiens |
| Cross-Linking-MS (XL-MS) |
|
Homo sapiens |
|
| 62 |
PICALM |
8301 |
| Co-fractionation |
|
Homo sapiens |
| Proximity Label-MS |
|
Homo sapiens |
|
| 63 |
MPDZ |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 64 |
SRPK1 |
6732 |
| Biochemical Activity |
|
Homo sapiens |
|
| 65 |
ANLN |
54443 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 66 |
NCKAP5L |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 67 |
DST |
667 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 68 |
CSNK1G3 |
1456 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 69 |
PALM2 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 70 |
EPB41L1 |
2036 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 71 |
ARHGAP32 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 72 |
FAM83B |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 73 |
FAM129B |
64855 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 74 |
Cd2ap |
12488 |
| Affinity Capture-MS |
|
Mus musculus |
|
| 75 |
SDHA |
6389 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 76 |
SCAMP1 |
9522 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 77 |
ERBB2IP |
55914 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 78 |
ANXA1 |
301 |
| Protein-peptide |
|
Homo sapiens |
|
| 79 |
DLG1 |
1739 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 80 |
GOLT1B |
51026 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 81 |
CCDC8 |
|
| Affinity Capture-MS |
|
Homo sapiens |
| Proximity Label-MS |
|
Homo sapiens |
|
| 82 |
MAP4K4 |
9448 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 83 |
LMTK2 |
22853 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 84 |
MYO19 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 85 |
TEAD2 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 86 |
FN1 |
2335 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 87 |
RPA3 |
6119 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 88 |
DLST |
1743 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 89 |
ABCC1 |
4363 |
| Co-fractionation |
|
Homo sapiens |
|
| 90 |
SORBS3 |
10174 |
| Reconstituted Complex |
|
Homo sapiens |
|
| 91 |
DZIP1 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 92 |
CRYBG3 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 93 |
CTNNA1 |
1495 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 94 |
KIDINS220 |
57498 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 95 |
USP6NL |
9712 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 96 |
IRF1 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 97 |
PDE8A |
5151 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 98 |
SOX2 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 99 |
STAT4 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 100 |
YBX1 |
4904 |
| Co-fractionation |
|
Homo sapiens |
|
| 101 |
GPRIN3 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 102 |
PSD3 |
23362 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 103 |
SHBG |
6462 |
| Two-hybrid |
|
Homo sapiens |
|
| 104 |
PLEC |
5339 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 105 |
MACF1 |
23499 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 106 |
LCK |
3932 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 107 |
S100A10 |
6281 |
| Co-fractionation |
|
Homo sapiens |
|
| 108 |
RAB11FIP1 |
80223 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 109 |
HM13 |
81502 |
| Co-fractionation |
|
Homo sapiens |
|
| 110 |
PHACTR4 |
65979 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 111 |
RAB11A |
8766 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 112 |
PLEKHA7 |
144100 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 113 |
EPHA2 |
1969 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 114 |
CUL7 |
9820 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 115 |
RAB5C |
5878 |
| Affinity Capture-MS |
|
Homo sapiens |
| Co-fractionation |
|
Homo sapiens |
|
| 116 |
MRPL55 |
|
| Co-fractionation |
|
Homo sapiens |
|
| 117 |
VANGL1 |
81839 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 118 |
NDUFS6 |
|
| Co-fractionation |
|
Homo sapiens |
|
| 119 |
CTNND1 |
1500 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 120 |
SEC61A1 |
29927 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 121 |
ARHGEF25 |
115557 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 122 |
Tmed2 |
56334 |
| Affinity Capture-MS |
|
Mus musculus |
|
| 123 |
IARS2 |
55699 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 124 |
Spast |
|
| Affinity Capture-MS |
|
Mus musculus |
|
| 125 |
FBXO7 |
25793 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 126 |
ARHGAP39 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 127 |
NOS1AP |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 128 |
NUP188 |
23511 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 129 |
RPA2 |
6118 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 130 |
APC |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 131 |
DBN1 |
1627 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 132 |
DTNA |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 133 |
MRPL50 |
54534 |
| Co-fractionation |
|
Homo sapiens |
|
| 134 |
VAMP2 |
6844 |
| Co-fractionation |
|
Homo sapiens |
| Proximity Label-MS |
|
Homo sapiens |
|
| 135 |
TBC1D8 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 136 |
CCDC102B |
|
| Two-hybrid |
|
Homo sapiens |
|
| 137 |
VANGL2 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 138 |
NME7 |
29922 |
| Two-hybrid |
|
Homo sapiens |
|
| 139 |
IQGAP1 |
8826 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 140 |
PRC1 |
9055 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 141 |
GPRC5B |
51704 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 142 |
OBSL1 |
23363 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 143 |
C1qbp |
12261 |
| Affinity Capture-MS |
|
Mus musculus |
|
| 144 |
Myh9 |
17886 |
| Affinity Capture-MS |
|
Mus musculus |
|
| 145 |
TMEM192 |
201931 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 146 |
PTPN14 |
5784 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 147 |
PALM |
5064 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 148 |
LHX4 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 149 |
SOX5 |
6660 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 150 |
Erh |
|
| Affinity Capture-MS |
|
Mus musculus |
|
| 151 |
STAM2 |
10254 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 152 |
JMJD6 |
23210 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 153 |
SLC25A10 |
1468 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 154 |
SLC1A5 |
6510 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 155 |
CEP55 |
55165 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 156 |
POLR3K |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 157 |
RAI14 |
26064 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 158 |
CBL |
867 |
| Reconstituted Complex |
|
Homo sapiens |
|
| 159 |
ADD2 |
119 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 160 |
TUBA1C |
84790 |
| Co-fractionation |
|
Homo sapiens |
|
| 161 |
SIPA1L2 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 162 |
KCNB2 |
|
| Proximity Label-MS |
|
Homo sapiens |
|
| 163 |
RAB2A |
5862 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 164 |
ATP6V1B1 |
525 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 165 |
SLC12A4 |
6560 |
| Co-fractionation |
|
Homo sapiens |
|
| 166 |
UBXN6 |
80700 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 167 |
TANC1 |
85461 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 168 |
MINK1 |
50488 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 169 |
PTOV1 |
|
| Affinity Capture-Western |
|
Homo sapiens |
| Co-fractionation |
|
Homo sapiens |
| Co-localization |
|
Homo sapiens |
|
| 170 |
DIP2B |
57609 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 171 |
RAB7A |
7879 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| 172 |
SOX6 |
|
| Affinity Capture-MS |
|
Homo sapiens |
|
| 173 |
Rmdn3 |
|
| Affinity Capture-MS |
|
Mus musculus |
|
| 174 |
TJP1 |
7082 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 175 |
RICTOR |
253260 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 176 |
USP54 |
159195 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 177 |
EGFR |
1956 |
| Proximity Label-MS |
|
Homo sapiens |
|
| 178 |
FAM90A1 |
|
| Two-hybrid |
|
Homo sapiens |
|
| 179 |
FTH1 |
2495 |
| Affinity Capture-MS |
|
Homo sapiens |
|
| | |