Gene ontology annotations for FLOT1 |
|
Experiment description of studies that identified FLOT1 in exosomes |
1 |
Experiment ID |
120 |
MISEV standards |
✘
|
Biophysical techniques |
✔
GAPDH|CD9|CD81|FLOT1
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
21362503
|
Organism |
Homo sapiens |
Experiment description |
Protein profile of exosomes from trabecular meshwork cells. |
Authors |
"Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL." |
Journal name |
JOP
|
Publication year |
2011 |
Sample |
Aqueous humor |
Sample name |
Aqueous humor - Normal |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
2 |
Experiment ID |
79 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|MHCII
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20458337
|
Organism |
Homo sapiens |
Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 |
Authors |
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." |
Journal name |
ICB
|
Publication year |
2010 |
Sample |
B cells |
Sample name |
RN (HLA-DR15) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [FT-ICR] Western blotting |
|
|
3 |
Experiment ID |
80 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|MHCII
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20458337
|
Organism |
Homo sapiens |
Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 |
Authors |
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." |
Journal name |
ICB
|
Publication year |
2010 |
Sample |
B cells |
Sample name |
RN (HLA-DR15) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [FT-ICR] Western blotting |
|
|
4 |
Experiment ID |
81 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|MHCII
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20458337
|
Organism |
Homo sapiens |
Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 |
Authors |
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." |
Journal name |
ICB
|
Publication year |
2010 |
Sample |
B cells |
Sample name |
RN (HLA-DR15) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [FT-ICR] Western blotting |
|
|
5 |
Experiment ID |
76 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20224111
|
Organism |
Homo sapiens |
Experiment description |
Proteomics analysis of bladder cancer exosomes. |
Authors |
"Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A." |
Journal name |
MCP
|
Publication year |
2010 |
Sample |
Bladder cancer cells |
Sample name |
HT1376 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.10-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS |
|
|
6 |
Experiment ID |
489 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 6 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
7 |
Experiment ID |
490 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
8 |
Experiment ID |
491 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 8 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
9 |
Experiment ID |
492 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 9 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
10 |
Experiment ID |
412 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MCF7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
11 |
Experiment ID |
414 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
12 |
Experiment ID |
426 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
13 |
Experiment ID |
426 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
14 |
Experiment ID |
426 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
15 |
Experiment ID |
427 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
16 |
Experiment ID |
429 |
MISEV standards |
✘
|
Biophysical techniques |
✔
FLOT1
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Fraction 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
17 |
Experiment ID |
430 |
MISEV standards |
✘
|
Biophysical techniques |
✔
FLOT1|SDCBP
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Fraction 3 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
18 |
Experiment ID |
431 |
MISEV standards |
✘
|
Biophysical techniques |
✔
FLOT1|SDCBP
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Fraction 4 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
19 |
Experiment ID |
432 |
MISEV standards |
✘
|
Biophysical techniques |
✔
FLOT1|SDCBP
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Fraction 5 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
20 |
Experiment ID |
433 |
MISEV standards |
✘
|
Biophysical techniques |
✔
FLOT1|SDCBP
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Fraction 6 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
21 |
Experiment ID |
522 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
FLOT1|CD9
|
Enriched markers |
✔
GOLGA2
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
34112803
|
Organism |
Homo sapiens |
Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
Journal name |
Nat Commun
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
22 |
Experiment ID |
523 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
FLOT1|CD9
|
Enriched markers |
✔
GOLGA2
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
34112803
|
Organism |
Homo sapiens |
Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
Journal name |
Nat Commun
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MCF7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
23 |
Experiment ID |
679 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
FLOT1|TSG101
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Western blotting
|
PubMed ID |
35939247
|
Organism |
Homo sapiens |
Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
Journal name |
Clin Exp Metastasis
|
Publication year |
2022 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231T |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
24 |
Experiment ID |
680 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
FLOT1|TSG101
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Western blotting
|
PubMed ID |
35939247
|
Organism |
Homo sapiens |
Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
Journal name |
Clin Exp Metastasis
|
Publication year |
2022 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231T |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
25 |
Experiment ID |
681 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
FLOT1|TSG101
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Western blotting
|
PubMed ID |
35939247
|
Organism |
Homo sapiens |
Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
Journal name |
Clin Exp Metastasis
|
Publication year |
2022 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-435 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
26 |
Experiment ID |
682 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
FLOT1|TSG101
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Western blotting
|
PubMed ID |
35939247
|
Organism |
Homo sapiens |
Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
Journal name |
Clin Exp Metastasis
|
Publication year |
2022 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-435 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
27 |
Experiment ID |
924 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|SDCBP|CD81|RAB35|LAMP2|FLOT1|TSG101|CD63
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
30918259
|
Organism |
Homo sapiens |
Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
Journal name |
Nat Commun
|
Publication year |
2019 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
28 |
Experiment ID |
926 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
30918259
|
Organism |
Homo sapiens |
Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
Journal name |
Nat Commun
|
Publication year |
2019 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
29 |
Experiment ID |
930 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
30918259
|
Organism |
Homo sapiens |
Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
Journal name |
Nat Commun
|
Publication year |
2019 |
Sample |
Breast cancer cells |
Sample name |
MCF7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
30 |
Experiment ID |
932 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD9|FLOT1|CD63
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
| | | | | |