Gene description for NEXN
Gene name nexilin (F actin binding protein)
Gene symbol NEXN
Other names/aliases CMH20
NELIN
Species Homo sapiens
 Database cross references - NEXN
ExoCarta ExoCarta_91624
Vesiclepedia VP_91624
Entrez Gene 91624
HGNC 29557
MIM 613121
UniProt Q0ZGT2  
 NEXN identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NEXN
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of muscle GO:0008307 IMP
    actin filament binding GO:0051015 IDA
    cell-cell adhesion mediator activity GO:0098632 IBA
Biological Process
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IBA
    axon guidance GO:0007411 IBA
    regulation of cell migration GO:0030334 IDA
    regulation of cytoskeleton organization GO:0051493 IEP
    dendrite self-avoidance GO:0070593 IBA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    adherens junction GO:0005912 IEA
    focal adhesion GO:0005925 HDA
    focal adhesion GO:0005925 IBA
    actin cytoskeleton GO:0015629 IDA
    Z disc GO:0030018 IBA
    Z disc GO:0030018 ISS
    axon GO:0030424 IBA
 Experiment description of studies that identified NEXN in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NEXN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRYGD  
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TRIM15  
PCA Homo sapiens
4 LIMA1 51474
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 FXR2 9513
Two-hybrid Homo sapiens
6 FBXO34  
Affinity Capture-MS Homo sapiens
7 CUL1 8454
Affinity Capture-MS Homo sapiens
8 Cdk1 12534
Affinity Capture-MS Mus musculus
9 Myh10 77579
Affinity Capture-MS Mus musculus
10 PPP1CB 5500
Affinity Capture-MS Homo sapiens
11 LGR4 55366
Affinity Capture-MS Homo sapiens
12 ACTA2 59
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 Myh9 17886
Affinity Capture-MS Mus musculus
14 WDR77 79084
Affinity Capture-MS Homo sapiens
15 Lima1  
Affinity Capture-MS Mus musculus
16 RPL23 9349
Affinity Capture-MS Homo sapiens
17 KIF14 9928
Affinity Capture-MS Homo sapiens
18 C6orf201  
Affinity Capture-MS Homo sapiens
19 ECT2 1894
Affinity Capture-MS Homo sapiens
20 EMCN  
Affinity Capture-MS Homo sapiens
21 C3orf36  
Affinity Capture-MS Homo sapiens
22 APOB 338
Cross-Linking-MS (XL-MS) Homo sapiens
23 Tmod3 50875
Affinity Capture-MS Mus musculus
24 Myo1c 17913
Affinity Capture-MS Mus musculus
25 DBN1 1627
Affinity Capture-MS Homo sapiens
26 SIRPA 140885
Reconstituted Complex Homo sapiens
27 EMB 133418
Affinity Capture-MS Homo sapiens
28 CALM1 801
Reconstituted Complex Homo sapiens
29 ACTBL2 345651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CAPZA2 830
Affinity Capture-MS Homo sapiens
31 CTTN 2017
Affinity Capture-MS Homo sapiens
32 HSPB1 3315
Two-hybrid Homo sapiens
33 MYO19  
Affinity Capture-MS Homo sapiens
34 RDX 5962
Cross-Linking-MS (XL-MS) Homo sapiens
35 PPP1R9A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 Tpx2  
Affinity Capture-MS Mus musculus
37 ACTB 60
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 Actb 11461
Affinity Capture-MS Mus musculus
39 MYO5C 55930
Affinity Capture-MS Homo sapiens
40 MYO1C 4641
Affinity Capture-MS Homo sapiens
41 MAPRE1 22919
Affinity Capture-MS Homo sapiens
42 MTMR10 54893
Affinity Capture-MS Homo sapiens
43 SLC39A3  
Affinity Capture-MS Homo sapiens
44 ZNF250  
Two-hybrid Homo sapiens
45 CAPZB 832
Affinity Capture-MS Homo sapiens
46 CPVL 54504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SYNPO 11346
Affinity Capture-MS Homo sapiens
48 MYO1D 4642
Affinity Capture-MS Homo sapiens
49 Flnb 286940
Affinity Capture-MS Mus musculus
50 MYC  
Affinity Capture-MS Homo sapiens
51 MYH9 4627
Affinity Capture-MS Homo sapiens
52 Trim69  
Affinity Capture-MS Mus musculus
53 ANK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 FLNA 2316
Affinity Capture-MS Homo sapiens
55 PINK1  
Affinity Capture-MS Homo sapiens
56 FBXO46  
Affinity Capture-MS Homo sapiens
57 CIT 11113
Affinity Capture-MS Homo sapiens
58 ACTR10 55860
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 CALD1 800
Affinity Capture-MS Homo sapiens
60 GSN 2934
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 Flot1 14251
Affinity Capture-MS Mus musculus
62 IQGAP1 8826
Affinity Capture-MS Homo sapiens
63 CUL7 9820
Affinity Capture-MS Homo sapiens
64 KRAS 3845
Synthetic Lethality Homo sapiens
65 CHMP4B 128866
Affinity Capture-MS Homo sapiens
66 Coro1c 23790
Affinity Capture-MS Mus musculus
67 OTUB2  
Affinity Capture-MS Homo sapiens
68 ACTC1 70
Affinity Capture-MS Homo sapiens
69 Tubgcp3  
Affinity Capture-MS Mus musculus
70 Flot2 14252
Affinity Capture-MS Mus musculus
71 MYO18A 399687
Affinity Capture-MS Homo sapiens
72 ZSCAN22  
Two-hybrid Homo sapiens
73 Tpm1 22003
Affinity Capture-MS Mus musculus
74 MYO1B 4430
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 Calml3  
Affinity Capture-MS Mus musculus
76 NXT1  
Affinity Capture-MS Homo sapiens
77 UBA52 7311
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NEXN is involved
No pathways found





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