Gene description for XRN2
Gene name 5'-3' exoribonuclease 2
Gene symbol XRN2
Other names/aliases -
Species Homo sapiens
 Database cross references - XRN2
ExoCarta ExoCarta_22803
Entrez Gene 22803
HGNC 12836
MIM 608851
UniProt Q9H0D6  
 XRN2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for XRN2
Molecular Function
    nuclease activity GO:0004518 TAS
    poly(A) RNA binding GO:0044822 IDA
    5'-3' exonuclease activity GO:0008409 IDA
    5'-3' exoribonuclease activity GO:0004534 TAS
    transcription termination site sequence-specific DNA binding GO:0001147 IDA
    zinc ion binding GO:0008270 IEA
Biological Process
    RNA metabolic process GO:0016070 ISS
    DNA catabolic process, exonucleolytic GO:0000738 IDA
    cell growth GO:0016049 ISS
    RNA phosphodiester bond hydrolysis, exonucleolytic GO:0090503 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
    DNA-templated transcription, termination GO:0006353 IEA
    spermatogenesis GO:0007283 IEP
    mRNA processing GO:0006397 IEA
    RNA catabolic process GO:0006401 TAS
    RNA processing GO:0006396 TAS
Subcellular Localization
    aggresome GO:0016235 IDA
    nucleolus GO:0005730 IDA
    membrane GO:0016020 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified XRN2 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for XRN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TARDBP 23435
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 SCFD1 23256
Affinity Capture-MS Homo sapiens
3 EIF5A 1984
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 EIF6 3692
Affinity Capture-MS Homo sapiens
5 MOCS3 27304
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
6 PSMA3 5684
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 UPF2  
Two-hybrid Homo sapiens
8 LSM3 27258
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 EEF1A1 1915
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 EXOSC10  
Two-hybrid Homo sapiens
11 COMT 1312
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
12 DXO  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 EIF3L 51386
Affinity Capture-MS Homo sapiens
14 TOLLIP 54472
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which XRN2 is involved
PathwayEvidenceSource
Association of TriC/CCT with target proteins during biosynthesis TAS Reactome





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