Gene description for PPP2CA
Gene name protein phosphatase 2, catalytic subunit, alpha isozyme
Gene symbol PPP2CA
Other names/aliases PP2Ac
PP2CA
PP2Calpha
RP-C
Species Homo sapiens
 Database cross references - PPP2CA
ExoCarta ExoCarta_5515
Entrez Gene 5515
HGNC 9299
MIM 176915
UniProt P67775  
 PPP2CA identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for PPP2CA
Molecular Function
    protein dimerization activity GO:0046983 IEA
    metal ion binding GO:0046872 IEA
    GABA receptor binding GO:0050811 IEA
    protein C-terminus binding GO:0008022 IEA
    protein binding GO:0005515 IPI
    protein serine/threonine phosphatase activity GO:0004722 IEA
Biological Process
    regulation of growth GO:0040008 NAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    mitotic nuclear envelope reassembly GO:0007084 TAS
    protein dephosphorylation GO:0006470 TAS
    negative regulation of epithelial to mesenchymal transition GO:0010719 IMP
    regulation of DNA replication GO:0006275 NAS
    regulation of Wnt signaling pathway GO:0030111 NAS
    regulation of protein catabolic process GO:0042176 IEA
    negative regulation of cell growth GO:0030308 NAS
    regulation of transcription, DNA-templated GO:0006355 NAS
    protein heterotrimerization GO:0070208 IEA
    regulation of cell differentiation GO:0045595 NAS
    regulation of cell adhesion GO:0030155 NAS
    negative regulation of tyrosine phosphorylation of Stat3 protein GO:0042518 NAS
    regulation of protein autophosphorylation GO:0031952 IEA
    mitotic cell cycle GO:0000278 TAS
    positive regulation of protein serine/threonine kinase activity GO:0071902 IMP
    RNA splicing GO:0008380 NAS
    apoptotic process GO:0006915 TAS
    second-messenger-mediated signaling GO:0019932 NAS
    response to organic substance GO:0010033 NAS
    inactivation of MAPK activity GO:0000188 NAS
    ceramide metabolic process GO:0006672 NAS
    regulation of receptor activity GO:0010469 IEA
    gene expression GO:0010467 TAS
    mesoderm development GO:0007498 IEA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    meiotic cell cycle GO:0051321 IEA
Subcellular Localization
    mitochondrion GO:0005739 NAS
    microtubule cytoskeleton GO:0015630 NAS
    membrane GO:0016020 NAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    spindle pole GO:0000922 IEA
    protein phosphatase type 2A complex GO:0000159 TAS
    nucleus GO:0005634 NAS
    extracellular exosome GO:0070062 IDA
    chromosome, centromeric region GO:0000775 IEA
 Experiment description of studies that identified PPP2CA in exosomes
1
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
7
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
8
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPP2CA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP2R5D  
Affinity Capture-MS Homo sapiens
2 STRIP1 85369
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TLX1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 TRIP13 9319
Two-hybrid Homo sapiens
5 PTN  
Two-hybrid Homo sapiens
6 PPP2R3A  
Two-hybrid Homo sapiens
7 ARL2 402
Co-purification Homo sapiens
8 PPP2R5A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 VDR  
Invivo Homo sapiens
10 STK24 8428
Affinity Capture-MS Homo sapiens
11 PPP2R5E 5529
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
12 STK25 10494
Affinity Capture-MS Homo sapiens
13 CCT3 7203
Affinity Capture-MS Homo sapiens
14 PXN  
Affinity Capture-Western Homo sapiens
15 FLJ21168  
Affinity Capture-MS Homo sapiens
16 CCT2 10576
Affinity Capture-MS Homo sapiens
17 PPM1B 5495
Co-purification Homo sapiens
18 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
19 PPP1CA 5499
Invivo Homo sapiens
Invitro Homo sapiens
20 PPFIA2 8499
Affinity Capture-MS Homo sapiens
21 TCP1 6950
Affinity Capture-MS Homo sapiens
22 STRN3 29966
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 STRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 RELA  
Reconstituted Complex Homo sapiens
25 VMD2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 ETF1 2107
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Two-hybrid Homo sapiens
27 CDK2 1017
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
28 DKFZp547A023  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PPP2R2C 5522
Affinity Capture-MS Homo sapiens
30 CDK6 1021
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
31 CCT6A 908
Affinity Capture-MS Homo sapiens
32 PREI3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PPP2R5B  
Affinity Capture-MS Homo sapiens
34 TBCD 6904
Affinity Capture-Western Homo sapiens
35 CCT7 10574
Affinity Capture-MS Homo sapiens
36 CCT5 22948
Affinity Capture-MS Homo sapiens
37 PDCD10 11235
Affinity Capture-MS Homo sapiens
38 STRN4 29888
Affinity Capture-MS Homo sapiens
39 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
40 PPP2R3B  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
41 STK26 51765
Affinity Capture-MS Homo sapiens
42 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 T3JAM  
Affinity Capture-MS Homo sapiens
44 MRPS26  
Two-hybrid Homo sapiens
45 CCT4 10575
Affinity Capture-MS Homo sapiens
46 ISYNA1 51477
Two-hybrid Homo sapiens
47 IGBP1  
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PPP2R5C 5527
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
49 TP53  
Affinity Capture-MS Homo sapiens
50 CCT8 10694
Affinity Capture-MS Homo sapiens
51 BCL2  
Invitro Homo sapiens
52 PPP2R2A 5520
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
53 PPFIA1 8500
Affinity Capture-MS Homo sapiens
54 PPP2R2D 55844
Affinity Capture-MS Homo sapiens
55 CCNG2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
56 CTTNBP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 FGFR1OP  
Affinity Capture-MS Homo sapiens
58 PPME1 51400
Affinity Capture-MS Homo sapiens
59 CDC5L 988
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
60 PPFIA3 8541
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPP2CA is involved
PathwayEvidenceSource
APC truncation mutants have impaired AXIN binding TAS Reactome
AXIN missense mutants destabilize the destruction complex TAS Reactome
Beta-catenin phosphorylation cascade TAS Reactome
CTLA4 inhibitory signaling TAS Reactome
Cyclin A/B1 associated events during G2/M transition TAS Reactome
Cyclin D associated events in G1 TAS Reactome
DARPP-32 events TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
disassembly of the destruction complex and recruitment of AXIN to the membrane TAS Reactome
ERK/MAPK targets TAS Reactome
ERK/MAPK targets TAS Reactome
ERKs are inactivated TAS Reactome
ERKs are inactivated TAS Reactome
Glycolysis TAS Reactome
Inhibition of replication initiation of damaged DNA by RB1/E2F1 TAS Reactome
Initiation of Nuclear Envelope Reformation TAS Reactome
Integration of energy metabolism TAS Reactome
MASTL Facilitates Mitotic Progression TAS Reactome
misspliced GSK3beta mutants stabilize beta-catenin TAS Reactome
Mitotic Prometaphase TAS Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome
Platelet sensitization by LDL TAS Reactome
PP2A-mediated dephosphorylation of key metabolic factors TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RHO GTPases Activate Formins TAS Reactome
S33 mutants of beta-catenin aren't phosphorylated TAS Reactome
S37 mutants of beta-catenin aren't phosphorylated TAS Reactome
S45 mutants of beta-catenin aren't phosphorylated TAS Reactome
Separation of Sister Chromatids TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Spry regulation of FGF signaling TAS Reactome
T41 mutants of beta-catenin aren't phosphorylated TAS Reactome
truncations of AMER1 destabilize the destruction complex TAS Reactome





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