Gene ontology annotations for RHOC |
|
Experiment description of studies that identified RHOC in exosomes |
1 |
Experiment ID |
489 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 6 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
2 |
Experiment ID |
490 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
3 |
Experiment ID |
491 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 8 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
4 |
Experiment ID |
492 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 9 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
5 |
Experiment ID |
426 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
6 |
Experiment ID |
427 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
7 |
Experiment ID |
494 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
35931686
|
Organism |
Homo sapiens |
Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
Authors |
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
Journal name |
Cell Death Dis
|
Publication year |
2022 |
Sample |
Chondrocytes |
Sample name |
Osteoarthritic cartilage |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
8 |
Experiment ID |
496 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
35931686
|
Organism |
Homo sapiens |
Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
Authors |
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
Journal name |
Cell Death Dis
|
Publication year |
2022 |
Sample |
Chondrocytes |
Sample name |
Healthy cartilage |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
9 |
Experiment ID |
497 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
35931686
|
Organism |
Homo sapiens |
Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
Authors |
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
Journal name |
Cell Death Dis
|
Publication year |
2022 |
Sample |
Chondrocytes |
Sample name |
T/C-28a2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
10 |
Experiment ID |
498 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
35931686
|
Organism |
Homo sapiens |
Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
Authors |
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
Journal name |
Cell Death Dis
|
Publication year |
2022 |
Sample |
Chondrocytes |
Sample name |
T/C-28a2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
11 |
Experiment ID |
21 |
MISEV standards |
✔
EM|IEM
|
Biophysical techniques |
✔
Alix|TSG101|HSP70|CD63
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
19837982
|
Organism |
Homo sapiens |
Experiment description |
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. |
Authors |
"Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" |
Journal name |
MCP
|
Publication year |
2009 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1215 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.10-1.12 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [Orbitrap] Western blotting |
|
|
12 |
Experiment ID |
207 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
✔
VDAC
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DKO-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
13 |
Experiment ID |
208 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
✔
VDAC
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
Dks-8 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
14 |
Experiment ID |
209 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
✔
VDAC
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DLD-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
15 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
16 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
17 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
18 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
19 |
Experiment ID |
363 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
20 |
Experiment ID |
364 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
21 |
Experiment ID |
365 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
22 |
Experiment ID |
126 |
MISEV standards |
✘
|
Biophysical techniques |
✔
GAPDH
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
PubMed ID |
Unpublished / Not applicable
|
Organism |
Homo sapiens |
Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
Authors |
"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" |
Journal name |
Mesenchymal Stem Cell Therapy
|
Publication year |
2011 |
Sample |
Mesenchymal stem cells |
Sample name |
huES9.E1 |
Isolation/purification methods |
HPLC |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Antobody array Mass spectrometry |
|
|
23 |
Experiment ID |
224 |
MISEV standards |
✔
EM|AFM
|
Biophysical techniques |
✔
Alix|TSG101|CD63|CD81
|
Enriched markers |
✔
GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25944692
|
Organism |
Homo sapiens |
Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
Authors |
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Neuroblastoma cells |
Sample name |
SH-SY5Y |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation OptiPrep density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
24 |
Experiment ID |
211 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
cytochrome c|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
IGROV1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
25 |
Experiment ID |
212 |
MISEV standards |
✔
CEM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
Cytochrome C|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
OVCAR-3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
26 |
Experiment ID |
434 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
27 |
Experiment ID |
435 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
28 |
Experiment ID |
622 |
MISEV standards |
✔
EM
|
Biophysical techniques |
|
Enriched markers |
✔
GOLGA2|CYC1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
29339034
|
Organism |
Homo sapiens |
Experiment description |
Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer |
Authors |
"Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR." |
Journal name |
HPB
|
Publication year |
2018 |
Sample |
Pancreatic duct fluid |
Sample name |
From patients with Pancreatic ductal adenocarcinoma |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
29 |
Experiment ID |
623 |
MISEV standards |
✘
|
Biophysical techniques |
|
Enriched markers |
✔
GOLGA2|CYC1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
29339034
|
Organism |
Homo sapiens |
Experiment description |
Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer |
Authors |
"Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR." |
Journal name |
HPB
|
Publication year |
2018 |
Sample |
Pancreatic duct fluid |
Sample name |
From patients with IPMN with microinvasive cancer (IPMNca) |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
30 |
Experiment ID |
231 |
MISEV standards |
✘
|
Biophysical techniques |
✔
Alix|CD63|CD9
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25332113
|
Organism |
Homo sapiens |
Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
Authors |
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." |
Journal name |
Transfusion
|
Publication year |
2015 |
Sample |
Platelets |
Sample name |
PL-Exs - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient |
Flotation density |
1.12-1.15 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
31 |
Experiment ID |
232 |
MISEV standards |
✘
|
Biophysical techniques |
✘
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25332113
|
Organism |
Homo sapiens |
Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
Authors |
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." |
Journal name |
Transfusion
|
Publication year |
2015 |
Sample |
Platelets |
Sample name |
PL-Exs - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient |
Flotation density |
1.12-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
32 |
Experiment ID |
233 |
MISEV standards |
✘
|
Biophysical techniques |
✘
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25332113
|
Organism |
Homo sapiens |
Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
Authors |
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." |
Journal name |
Transfusion
|
Publication year |
2015 |
Sample |
Platelets |
Sample name |
PL-Exs - Rep 3 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient |
Flotation density |
1.12-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
33 |
Experiment ID |
834 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
35333565
|
Organism |
Homo sapiens |
Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
Journal name |
Sci Adv
|
Publication year |
2022 |
Sample |
Retinal pigment epithelial cells |
Sample name |
ARPE-19 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
34 |
Experiment ID |
835 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
35333565
|
Organism |
Homo sapiens |
Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
Journal name |
Sci Adv
|
Publication year |
2022 |
Sample |
Retinal pigment epithelial cells |
Sample name |
ARPE-19 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
35 |
Experiment ID |
66 |
MISEV standards |
✔
IEM
|
Biophysical techniques |
✔
TSG101|Alix|CD63|CD81
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19199708
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). |
Authors |
"Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR" |
Journal name |
JPR
|
Publication year |
2009 |
Sample |
Saliva |
Sample name |
Saliva |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy |
|
|
36 |
Experiment ID |
191 |
MISEV standards |
✘
|
Biophysical techniques |
✔
Alix|CD81|CD9
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20124223
|
Organism |
Homo sapiens |
Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
Authors |
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK." |
Journal name |
Mol Cell Proteomics
|
Publication year |
2010 |
Sample |
Squamous carcinoma cells |
Sample name |
Squamous carcinoma cell (A431) |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
37 |
Experiment ID |
217 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|CD81|CD9|CD63
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23844026
|
Organism |
Homo sapiens |
Experiment description |
Characterization of human thymic exosomes. |
Authors |
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." |
Journal name |
PLoS One
|
Publication year |
2013 |
Sample |
Thymus |
Sample name |
Normal-Thymus |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
38 |
Experiment ID |
63 |
MISEV standards |
✘
|
Biophysical techniques |
✔
AQP2
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19056867
|
Organism |
Homo sapiens |
Experiment description |
Large-scale proteomics and phosphoproteomics of urinary exosomes. |
Authors |
"Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" |
Journal name |
JASN
|
Publication year |
2009 |
Sample |
Urine |
Sample name |
Urine - Normal |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting |
|
|
39 |
Experiment ID |
196 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Alix|TSG101|HSP70|CD9
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22418980
|
Organism |
Homo sapiens |
Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
Authors |
"Raj DA, Fiume I, Capasso G, Pocsfalvi G." |
Journal name |
Kidney Int
|
Publication year |
2012 |
Sample |
Urine |
Sample name |
Urine - Normal high density |
Isolation/purification methods |
Differential centrifugation Sucrose cushion |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
40 |
Experiment ID |
197 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Alix|TSG101|HSP70|CD9
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22418980
|
Organism |
Homo sapiens |
Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
Authors |
"Raj DA, Fiume I, Capasso G, Pocsfalvi G." |
Journal name |
Kidney Int
|
Publication year |
2012 |
Sample |
Urine |
Sample name |
Urine - Normal low density |
Isolation/purification methods |
Differential centrifugation Sucrose cushion |
| | | |