Gene description for ARL1
Gene name ADP-ribosylation factor-like 1
Gene symbol ARL1
Other names/aliases ARFL1
Species Homo sapiens
 Database cross references - ARL1
ExoCarta ExoCarta_400
Vesiclepedia VP_400
Entrez Gene 400
HGNC 692
MIM 603425
UniProt P40616  
 ARL1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for ARL1
Molecular Function
    GTPase activity GO:0003924 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    enzyme activator activity GO:0008047 IDA
    protein domain specific binding GO:0019904 IPI
    metal ion binding GO:0046872 IEA
    phospholipase D activator activity GO:1990583 IDA
Biological Process
    intracellular protein transport GO:0006886 IBA
    Golgi organization GO:0007030 ISS
    toxin metabolic process GO:0009404 IDA
    vesicle-mediated transport GO:0016192 IBA
    protein localization to Golgi apparatus GO:0034067 IMP
    retrograde transport, endosome to Golgi GO:0042147 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IDA
    trans-Golgi network GO:0005802 ISS
    cytosol GO:0005829 TAS
    trans-Golgi network membrane GO:0032588 TAS
 Experiment description of studies that identified ARL1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 GOLGA4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 GCC1 79571
Two-hybrid Homo sapiens
5 DUSP22  
Affinity Capture-MS Homo sapiens
6 ACTR2 10097
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 FASN 2194
Negative Genetic Homo sapiens
9 ARFIP1 27236
Affinity Capture-MS Homo sapiens
10 PARP1 142
Proximity Label-MS Homo sapiens
11 COIL  
Proximity Label-MS Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 ATG9A 79065
Proximity Label-MS Homo sapiens
14 YWHAB 7529
Affinity Capture-MS Homo sapiens
15 ECT2 1894
Affinity Capture-MS Homo sapiens
16 SMYD2 56950
Affinity Capture-MS Homo sapiens
17 MME 4311
Affinity Capture-MS Homo sapiens
18 UFL1 23376
Affinity Capture-MS Homo sapiens
19 DNAJB2 3300
Affinity Capture-MS Homo sapiens
20 ASB1 51665
Affinity Capture-MS Homo sapiens
21 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 YWHAZ 7534
Affinity Capture-MS Homo sapiens
23 SQSTM1 8878
Proximity Label-MS Homo sapiens
24 C9orf72  
Affinity Capture-MS Homo sapiens
25 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 RPP30 10556
Affinity Capture-MS Homo sapiens
27 CHCHD4  
Affinity Capture-MS Homo sapiens
28 C16orf72 29035
Affinity Capture-MS Homo sapiens
29 PTPN1 5770
Affinity Capture-MS Homo sapiens
30 ARFIP2 23647
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
31 MKI67  
Affinity Capture-MS Homo sapiens
32 STAU1 6780
Affinity Capture-MS Homo sapiens
33 EXOC4 60412
Affinity Capture-MS Homo sapiens
34 YWHAQ 10971
Affinity Capture-MS Homo sapiens
35 AURKB 9212
Affinity Capture-MS Homo sapiens
36 KIAA1429 25962
Affinity Capture-MS Homo sapiens
37 RRAS2 22800
Affinity Capture-MS Homo sapiens
38 PTPN5  
Affinity Capture-MS Homo sapiens
39 YWHAE 7531
Affinity Capture-MS Homo sapiens
40 TBC1D32 221322
Proximity Label-MS Homo sapiens
41 ARL3 403
Affinity Capture-MS Homo sapiens
42 MYC  
Affinity Capture-MS Homo sapiens
43 RPA2 6118
Proximity Label-MS Homo sapiens
44 NEDD1 121441
Affinity Capture-MS Homo sapiens
45 TRAPPC2 6399
Affinity Capture-MS Homo sapiens
46 GOLGA1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
47 CDK5RAP3 80279
Affinity Capture-MS Homo sapiens
48 PRC1 9055
Affinity Capture-MS Homo sapiens
49 ASB10  
Affinity Capture-MS Homo sapiens
50 PTPN23 25930
Affinity Capture-MS Homo sapiens
51 ACTB 60
Affinity Capture-MS Homo sapiens
52 DDRGK1 65992
Affinity Capture-MS Homo sapiens
53 RNF2  
Affinity Capture-MS Homo sapiens
54 SLC9A9 285195
Affinity Capture-MS Homo sapiens
55 KRAS 3845
Affinity Capture-MS Homo sapiens
56 SREBF2 6721
Negative Genetic Homo sapiens
57 FSCN1 6624
Affinity Capture-MS Homo sapiens
58 PTPN18 26469
Affinity Capture-MS Homo sapiens
59 ARHGAP31 57514
Affinity Capture-MS Homo sapiens
60 RPA3 6119
Proximity Label-MS Homo sapiens
61 PTPRO 5800
Affinity Capture-MS Homo sapiens
62 Ksr1  
Affinity Capture-MS Mus musculus
63 GCC2 9648
Two-hybrid Homo sapiens
64 HUWE1 10075
Affinity Capture-MS Homo sapiens
65 DRD2  
Two-hybrid Homo sapiens
66 HIF1A 3091
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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