Gene description for TPRN
Gene name taperin
Gene symbol TPRN
Other names/aliases C9orf75
DFNB79
Species Homo sapiens
 Database cross references - TPRN
ExoCarta ExoCarta_286262
Vesiclepedia VP_286262
Entrez Gene 286262
HGNC 26894
MIM 613354
UniProt Q4KMQ1  
 TPRN identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for TPRN
Molecular Function
    actin binding GO:0003779 ISS
    protein serine/threonine phosphatase inhibitor activity GO:0004865 IDA
    protein binding GO:0005515 IPI
    protein phosphatase 1 binding GO:0008157 IPI
Biological Process
    sensory perception of sound GO:0007605 IBA
    sensory perception of sound GO:0007605 IMP
    sensory perception of sound GO:0007605 ISS
    auditory receptor cell stereocilium organization GO:0060088 IBA
    stereocilium maintenance GO:0120045 IBA
    stereocilium maintenance GO:0120045 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    microvillus GO:0005902 ISS
    stereocilium GO:0032420 ISS
    stereocilium base GO:0120044 IBA
    stereocilium base GO:0120044 ISS
 Experiment description of studies that identified TPRN in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TPRN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL9 55958
Affinity Capture-MS Homo sapiens
2 FTL 2512
Affinity Capture-MS Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 OGFOD1  
Affinity Capture-MS Homo sapiens
5 LIMA1 51474
Affinity Capture-MS Homo sapiens
6 PDLIM7 9260
Affinity Capture-MS Homo sapiens
7 Myh10 77579
Affinity Capture-MS Mus musculus
8 PPP1CB 5500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PPP1R3A  
Affinity Capture-MS Homo sapiens
10 SMARCA2 6595
Affinity Capture-MS Homo sapiens
11 PPP1R2 5504
Affinity Capture-MS Homo sapiens
12 Myh9 17886
Affinity Capture-MS Mus musculus
13 Lima1  
Affinity Capture-MS Mus musculus
14 KIF14 9928
Affinity Capture-MS Homo sapiens
15 ECT2 1894
Affinity Capture-MS Homo sapiens
16 CHMP4C 92421
Affinity Capture-MS Homo sapiens
17 MYO15B 80022
Two-hybrid Homo sapiens
18 Kif18a  
Affinity Capture-MS Mus musculus
19 PPP1CA 5499
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 Myo1c 17913
Affinity Capture-MS Mus musculus
21 DBN1 1627
Affinity Capture-MS Homo sapiens
22 Itga5 16402
Affinity Capture-MS Mus musculus
23 CAPZA2 830
Affinity Capture-MS Homo sapiens
24 EPS8 2059
Two-hybrid Homo sapiens
25 MYO19  
Affinity Capture-MS Homo sapiens
26 Actb 11461
Affinity Capture-MS Mus musculus
27 MYO5C 55930
Affinity Capture-MS Homo sapiens
28 MYO1C 4641
Affinity Capture-MS Homo sapiens
29 MAPRE1 22919
Affinity Capture-MS Homo sapiens
30 CLIC5 53405
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
31 TLR9  
Affinity Capture-MS Homo sapiens
32 PPP1CC 5501
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
33 TFDP2  
Affinity Capture-MS Homo sapiens
34 SYNPO 11346
Affinity Capture-MS Homo sapiens
35 Flnb 286940
Affinity Capture-MS Mus musculus
36 MYC  
Affinity Capture-MS Homo sapiens
37 MYH9 4627
Affinity Capture-MS Homo sapiens
38 CLIC1 1192
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
39 FLNA 2316
Affinity Capture-MS Homo sapiens
40 Heatr2  
Affinity Capture-MS Mus musculus
41 CIT 11113
Affinity Capture-MS Homo sapiens
42 Flot1 14251
Affinity Capture-MS Mus musculus
43 IQGAP1 8826
Affinity Capture-MS Homo sapiens
44 CAMK2D 817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 Coro1c 23790
Affinity Capture-MS Mus musculus
46 QRICH1  
Affinity Capture-MS Homo sapiens
47 Flot2 14252
Affinity Capture-MS Mus musculus
48 CLIC4 25932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
49 Chmp4b 75608
Affinity Capture-MS Mus musculus
50 CLIC2 1193
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
51 MYO18A 399687
Affinity Capture-MS Homo sapiens
52 Tpm1 22003
Affinity Capture-MS Mus musculus
53 Calml3  
Affinity Capture-MS Mus musculus
54 PIPSL 266971
Affinity Capture-MS Homo sapiens
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