Experiment description of studies that identified HSPA7 in sEVs

1 Experiment ID 20 MISEV standards ✔ EM Biophysical techniques ✔ HSP90|CD63|CD81|LAMP1 Enriched markers ✔ GOLGA2|cytochrome c Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17956143     Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells. Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS" Journal name JPR Publication year 2007 Sample Colorectal cancer cells Sample name HT29 Isolation/purification methods Differential centrifugation Sucrose density gradient Diafiltration Flotation density 1.16 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

2 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

3 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for HSPA7

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Affinity Capture-MS Homo sapiens 2 ANLN 54443 Affinity Capture-MS Homo sapiens 3 CHMP4C 92421 Affinity Capture-MS Homo sapiens 4 METTL18   Affinity Capture-MS Homo sapiens 5 CUL1 8454 Affinity Capture-MS Homo sapiens 6 TFCP2 7024 Affinity Capture-MS Homo sapiens 7 SERBP1 26135 Affinity Capture-MS Homo sapiens 8 KIF14 9928 Affinity Capture-MS Homo sapiens 9 TUBG1 7283 Affinity Capture-MS Homo sapiens 10 KCTD10 83892 Affinity Capture-MS Homo sapiens 11 MCM5 4174 Affinity Capture-MS Homo sapiens 12 CUL5 8065 Affinity Capture-MS Homo sapiens 13 KIF20A 10112 Affinity Capture-MS Homo sapiens 14 ECT2 1894 Affinity Capture-MS Homo sapiens 15 CAND1 55832 Affinity Capture-MS Homo sapiens 16 PARK2   Affinity Capture-MS Homo sapiens 17 UBXN11   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 HSP90AA1 3320 Affinity Capture-MS Homo sapiens 19 METTL21B   Affinity Capture-MS Homo sapiens 20 CUL4B 8450 Affinity Capture-MS Homo sapiens 21 AURKB 9212 Affinity Capture-MS Homo sapiens 22 WTAP 9589 Affinity Capture-MS Homo sapiens 23 CEP250 11190 Affinity Capture-MS Homo sapiens 24 UBASH3B 84959 Affinity Capture-MS Homo sapiens 25 DCUN1D1 54165 Affinity Capture-MS Homo sapiens 26 CHMP4B 128866 Affinity Capture-MS Homo sapiens 27 PPP2R1A 5518 Affinity Capture-MS Homo sapiens 28 YAP1 10413 Affinity Capture-MS Homo sapiens 29 HSPA2 3306 Affinity Capture-MS Homo sapiens 30 ARRB1 408 Affinity Capture-MS Homo sapiens 31 KIF23 9493 Affinity Capture-MS Homo sapiens 32 MCM2 4171 Affinity Capture-MS Homo sapiens 33 COPS6 10980 Affinity Capture-MS Homo sapiens 34 METTL21A   Affinity Capture-MS Homo sapiens 35 CUL4A 8451 Affinity Capture-MS Homo sapiens 36 KMO   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 37 COPS5 10987 Affinity Capture-MS Homo sapiens 38 PRC1 9055 Affinity Capture-MS Homo sapiens 39 CRY2   Affinity Capture-MS Homo sapiens 40 CUL2 8453 Affinity Capture-MS Homo sapiens 41 NEDD8 4738 Affinity Capture-MS Homo sapiens View the network

Pathways in which HSPA7 is involved

No pathways found