Gene description for AAAS
Gene name achalasia, adrenocortical insufficiency, alacrimia
Gene symbol AAAS
Other names/aliases AAA
AAASb
ADRACALA
ADRACALIN
ALADIN
GL003
Species Homo sapiens
 Database cross references - AAAS
ExoCarta ExoCarta_8086
Vesiclepedia VP_8086
Entrez Gene 8086
HGNC 13666
MIM 605378
UniProt Q9NRG9  
 AAAS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for AAAS
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    microtubule bundle formation GO:0001578 IMP
    glutathione metabolic process GO:0006749 IEA
    nucleocytoplasmic transport GO:0006913 IBA
    nucleocytoplasmic transport GO:0006913 IDA
    nucleocytoplasmic transport GO:0006913 NAS
    response to oxidative stress GO:0006979 IEA
    learning GO:0007612 IEA
    fertilization GO:0009566 IEA
    protein transport GO:0015031 IEA
    multicellular organism growth GO:0035264 IEA
    regulation of nucleocytoplasmic transport GO:0046822 NAS
    mRNA transport GO:0051028 IEA
    mitotic spindle assembly GO:0090307 IMP
Subcellular Localization
    spindle pole GO:0000922 IDA
    nucleus GO:0005634 HDA
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 TAS
    nuclear pore GO:0005643 IBA
    nuclear pore GO:0005643 IDA
    nuclear pore GO:0005643 NAS
    nucleoplasm GO:0005654 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    nuclear membrane GO:0031965 IDA
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified AAAS in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AAAS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 CIB2  
Affinity Capture-MS Homo sapiens
5 METTL7A 25840
Proximity Label-MS Homo sapiens
6 LAMP3  
Proximity Label-MS Homo sapiens
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 SLC39A4 55630
Affinity Capture-MS Homo sapiens
9 REL 5966
Two-hybrid Homo sapiens
10 KPNB1 3837
Affinity Capture-MS Homo sapiens
11 CTDNEP1 23399
Proximity Label-MS Homo sapiens
12 MDFI  
Affinity Capture-MS Homo sapiens
13 KIF20A 10112
Affinity Capture-MS Homo sapiens
14 HSD17B11 51170
Proximity Label-MS Homo sapiens
15 LOC100132735  
Protein-RNA Homo sapiens
16 PTPN1 5770
Proximity Label-MS Homo sapiens
17 LYPD3 27076
Affinity Capture-MS Homo sapiens
18 NUP153 9972
Affinity Capture-MS Homo sapiens
19 SSR1 6745
Proximity Label-MS Homo sapiens
20 OBSL1 23363
Affinity Capture-MS Homo sapiens
21 PIGH  
Affinity Capture-MS Homo sapiens
22 JTB 10899
Affinity Capture-MS Homo sapiens
23 ABHD14A  
Affinity Capture-MS Homo sapiens
24 SLC25A46 91137
Proximity Label-MS Homo sapiens
25 SNX27 81609
Affinity Capture-MS Homo sapiens
26 MARCKS 4082
Proximity Label-MS Homo sapiens
27 NUP155 9631
Proximity Label-MS Homo sapiens
28 CHMP4C 92421
Affinity Capture-MS Homo sapiens
29 FAM96A  
Affinity Capture-MS Homo sapiens
30 MTCH2 23788
Proximity Label-MS Homo sapiens
31 Rcc1  
Affinity Capture-MS Mus musculus
32 EMD 2010
Proximity Label-MS Homo sapiens
33 USP11 8237
Affinity Capture-MS Homo sapiens
34 RB1CC1 9821
Affinity Capture-MS Homo sapiens
35 PARK2  
Affinity Capture-MS Homo sapiens
36 EMP3 2014
Affinity Capture-MS Homo sapiens
37 SCAP  
Affinity Capture-MS Homo sapiens
38 UBE2I 7329
Affinity Capture-MS Homo sapiens
39 NT5C 30833
Affinity Capture-MS Homo sapiens
40 NTRK1 4914
Affinity Capture-MS Homo sapiens
41 RPN1 6184
Proximity Label-MS Homo sapiens
42 Ube2i  
Affinity Capture-MS Mus musculus
43 RHOT2 89941
Proximity Label-MS Homo sapiens
44 SEC61B 10952
Proximity Label-MS Homo sapiens
45 UBXN6 80700
Affinity Capture-MS Homo sapiens
46 REEP5 7905
Proximity Label-MS Homo sapiens
47 ANLN 54443
Affinity Capture-MS Homo sapiens
48 RPN2 6185
Proximity Label-MS Homo sapiens
49 Nup98  
Affinity Capture-MS Mus musculus
50 OTUD1 220213
Affinity Capture-MS Homo sapiens
51 XPO1 7514
Affinity Capture-MS Homo sapiens
52 PANX1 24145
Proximity Label-MS Homo sapiens
53 ECT2 1894
Affinity Capture-MS Homo sapiens
54 CRELD1 78987
Affinity Capture-MS Homo sapiens
55 BCAP31 10134
Proximity Label-MS Homo sapiens
56 HSD3B7 80270
Proximity Label-MS Homo sapiens
57 NR3C1 2908
Proximity Label-MS Homo sapiens
58 EGFR 1956
Affinity Capture-MS Homo sapiens
59 FKBP8 23770
Proximity Label-MS Homo sapiens
60 LOR  
Affinity Capture-MS Homo sapiens
61 CA6 765
Affinity Capture-MS Homo sapiens
62 MAPK6  
Affinity Capture-MS Homo sapiens
63 C12orf74  
Affinity Capture-MS Homo sapiens
64 Nup107  
Affinity Capture-MS Mus musculus
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 SIRT6  
Affinity Capture-MS Homo sapiens
67 SEH1L 81929
Affinity Capture-MS Homo sapiens
68 EFNB2 1948
Affinity Capture-MS Homo sapiens
69 AKAP1 8165
Proximity Label-MS Homo sapiens
70 HUWE1 10075
Affinity Capture-MS Homo sapiens
71 CLPP 8192
Proximity Label-MS Homo sapiens
72 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
73 FASN 2194
Positive Genetic Homo sapiens
74 TMEM9 252839
Affinity Capture-MS Homo sapiens
75 BKRF1  
Affinity Capture-MS
76 OPALIN  
Affinity Capture-MS Homo sapiens
77 UVSSA  
Affinity Capture-MS Homo sapiens
78 SQSTM1 8878
Proximity Label-MS Homo sapiens
79 NUP35 129401
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
81 NUP107 57122
Affinity Capture-MS Homo sapiens
82 SEC62 7095
Proximity Label-MS Homo sapiens
83 NDC1 55706
Affinity Capture-MS Homo sapiens
84 RAB7A 7879
Proximity Label-MS Homo sapiens
85 CIT 11113
Affinity Capture-MS Homo sapiens
86 Ranbp2  
Affinity Capture-MS Mus musculus
87 CUL7 9820
Affinity Capture-MS Homo sapiens
88 C9orf72  
Affinity Capture-MS Homo sapiens
89 AR 367
Affinity Capture-MS Homo sapiens
90 PLD6  
Affinity Capture-MS Homo sapiens
91 GNAI3 2773
Affinity Capture-MS Homo sapiens
92 DERL1 79139
Proximity Label-MS Homo sapiens
93 POPDC2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AAAS is involved
PathwayEvidenceSource
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Disorders of transmembrane transporters TAS Reactome
Export of Viral Ribonucleoproteins from Nucleus TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
HCMV Late Events TAS Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle TAS Reactome
Host Interactions of HIV factors IEA Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Interactions of Rev with host cellular proteins IEA Reactome
Interactions of Rev with host cellular proteins TAS Reactome
Interactions of Vpr with host cellular proteins TAS Reactome
Interferon Signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome
Late Phase of HIV Life Cycle TAS Reactome
M Phase TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of non-coding RNA TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Mitotic Prophase TAS Reactome
NEP/NS2 Interacts with the Cellular Export Machinery TAS Reactome
NS1 Mediated Effects on Host Pathways TAS Reactome
Nuclear Envelope Breakdown TAS Reactome
Nuclear import of Rev protein IEA Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Rev-mediated nuclear export of HIV RNA TAS Reactome
RHO GTPase cycle TAS Reactome
RHOA GTPase cycle TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
Signal Transduction TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
SLC transporter disorders TAS Reactome
snRNP Assembly TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation TAS Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of DNA replication proteins TAS Reactome
SUMOylation of RNA binding proteins TAS Reactome
SUMOylation of SUMOylation proteins TAS Reactome
SUMOylation of ubiquitinylation proteins TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript TAS Reactome
Transport of Mature mRNA Derived from an Intronless Transcript TAS Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts TAS Reactome
Transport of Mature Transcript to Cytoplasm TAS Reactome
Transport of Ribonucleoproteins into the Host Nucleus TAS Reactome
Transport of the SLBP Dependant Mature mRNA TAS Reactome
Transport of the SLBP independent Mature mRNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Messenger RNA Synthesis TAS Reactome
Vpr-mediated nuclear import of PICs TAS Reactome





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