Gene description for RPL13A
Gene name ribosomal protein L13a
Gene symbol RPL13A
Other names/aliases L13A
TSTA1
Species Homo sapiens
 Database cross references - RPL13A
ExoCarta ExoCarta_23521
Entrez Gene 23521
HGNC 10304
UniProt P40429  
 RPL13A identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for RPL13A
Molecular Function
    structural constituent of ribosome GO:0003735 NAS
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    negative regulation of formation of translation preinitiation complex GO:1901194 IDA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 TAS
    gene expression GO:0010467 TAS
    translational elongation GO:0006414 TAS
    cellular protein metabolic process GO:0044267 TAS
    viral transcription GO:0019083 TAS
    viral life cycle GO:0019058 TAS
    cellular response to interferon-gamma GO:0071346 IDA
    translational initiation GO:0006413 TAS
    translation GO:0006412 TAS
    viral process GO:0016032 TAS
    translational termination GO:0006415 TAS
    negative regulation of translation GO:0017148 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    large ribosomal subunit GO:0015934 TAS
    nucleolus GO:0005730 IDA
    GAIT complex GO:0097452 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IDA
    ribonucleoprotein complex GO:0030529 IDA
    cytosolic large ribosomal subunit GO:0022625 IDA
    membrane GO:0016020 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified RPL13A in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPL13A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GOLM1 51280
Two-hybrid Homo sapiens
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